Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides recC and ptrA are separated by 175 nucleotides
OKFHMN_20025: pulJ - prepilin peptidase-dependent protein, at 3,890,274 to 3,890,837
pulJ
OKFHMN_20030: ygdB - DUF2509 domain-containing protein, at 3,890,834 to 3,891,241
ygdB
OKFHMN_20035: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 3,891,226 to 3,891,549
ppdC
OKFHMN_20040: recC - exodeoxyribonuclease V subunit gamma, at 3,891,562 to 3,894,930
recC
OKFHMN_20045: ptrA - pitrilysin, at 3,895,106 to 3,897,994
ptrA
Position (kb)
3891
3892
3893
3894
3895 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3890.562 kb on - strand, within pulJ at 3890.562 kb on - strand, within pulJ at 3890.699 kb on + strand, within pulJ at 3890.720 kb on - strand, within pulJ at 3890.762 kb on + strand, within pulJ at 3890.811 kb on + strand at 3890.811 kb on + strand at 3890.812 kb on - strand at 3890.901 kb on + strand, within ygdB at 3891.049 kb on - strand, within ygdB at 3891.108 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.112 kb on - strand, within ygdB at 3891.226 kb on - strand at 3891.336 kb on - strand, within ppdC at 3891.349 kb on - strand, within ppdC at 3891.982 kb on + strand, within recC at 3894.964 kb on - strand at 3894.968 kb on + strand at 3894.998 kb on + strand at 3894.999 kb on - strand at 3895.000 kb on + strand at 3895.000 kb on + strand at 3895.000 kb on + strand at 3895.001 kb on - strand at 3895.002 kb on + strand at 3895.002 kb on + strand at 3895.002 kb on + strand at 3895.002 kb on + strand at 3895.003 kb on - strand at 3895.008 kb on + strand at 3895.134 kb on + strand at 3895.135 kb on - strand at 3895.143 kb on + strand at 3895.143 kb on + strand at 3895.143 kb on + strand at 3895.144 kb on - strand at 3895.164 kb on + strand at 3895.165 kb on - strand at 3895.219 kb on + strand at 3895.220 kb on - strand at 3895.243 kb on + strand at 3895.245 kb on + strand at 3895.245 kb on + strand at 3895.246 kb on - strand at 3895.370 kb on + strand at 3895.371 kb on - strand at 3895.381 kb on + strand at 3895.382 kb on - strand at 3895.382 kb on - strand at 3895.454 kb on - strand, within ptrA at 3895.531 kb on - strand, within ptrA at 3895.595 kb on - strand, within ptrA at 3895.708 kb on + strand, within ptrA at 3895.778 kb on + strand, within ptrA at 3895.779 kb on - strand, within ptrA at 3895.782 kb on - strand, within ptrA at 3895.816 kb on + strand, within ptrA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,890,562 - pulJ OKFHMN_20025 0.51 +0.5 3,890,562 - pulJ OKFHMN_20025 0.51 +1.3 3,890,699 + pulJ OKFHMN_20025 0.75 +0.2 3,890,720 - pulJ OKFHMN_20025 0.79 -0.5 3,890,762 + pulJ OKFHMN_20025 0.87 -0.7 3,890,811 + -0.1 3,890,811 + -1.6 3,890,812 - -1.4 3,890,901 + ygdB OKFHMN_20030 0.16 -2.3 3,891,049 - ygdB OKFHMN_20030 0.53 +0.4 3,891,108 + ygdB OKFHMN_20030 0.67 -0.2 3,891,111 + ygdB OKFHMN_20030 0.68 +0.2 3,891,111 + ygdB OKFHMN_20030 0.68 +0.6 3,891,111 + ygdB OKFHMN_20030 0.68 -2.4 3,891,112 - ygdB OKFHMN_20030 0.68 +1.0 3,891,226 - +0.5 3,891,336 - ppdC OKFHMN_20035 0.34 -1.7 3,891,349 - ppdC OKFHMN_20035 0.38 -1.4 3,891,982 + recC OKFHMN_20040 0.12 -0.9 3,894,964 - +0.3 3,894,968 + +0.5 3,894,998 + +0.2 3,894,999 - +0.6 3,895,000 + -0.0 3,895,000 + +0.3 3,895,000 + +0.9 3,895,001 - +0.4 3,895,002 + +0.1 3,895,002 + -0.9 3,895,002 + -0.4 3,895,002 + +1.0 3,895,003 - +0.9 3,895,008 + +1.0 3,895,134 + -1.2 3,895,135 - -0.8 3,895,143 + -0.2 3,895,143 + +2.1 3,895,143 + +0.3 3,895,144 - +0.7 3,895,164 + +0.0 3,895,165 - +0.0 3,895,219 + -0.8 3,895,220 - -0.7 3,895,243 + -1.3 3,895,245 + +0.0 3,895,245 + +0.6 3,895,246 - +0.5 3,895,370 + +1.6 3,895,371 - -0.3 3,895,381 + -1.4 3,895,382 - +0.0 3,895,382 - -1.3 3,895,454 - ptrA OKFHMN_20045 0.12 +1.1 3,895,531 - ptrA OKFHMN_20045 0.15 -0.4 3,895,595 - ptrA OKFHMN_20045 0.17 +0.4 3,895,708 + ptrA OKFHMN_20045 0.21 +1.5 3,895,778 + ptrA OKFHMN_20045 0.23 +0.1 3,895,779 - ptrA OKFHMN_20045 0.23 +0.5 3,895,782 - ptrA OKFHMN_20045 0.23 -0.4 3,895,816 + ptrA OKFHMN_20045 0.25 +0.2
Or see this region's nucleotide sequence