Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppdA and pulJ overlap by 10 nucleotides pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides
OKFHMN_20020: ppdA - prepilin peptidase-dependent protein, at 3,889,813 to 3,890,283
ppdA
OKFHMN_20025: pulJ - prepilin peptidase-dependent protein, at 3,890,274 to 3,890,837
pulJ
OKFHMN_20030: ygdB - DUF2509 domain-containing protein, at 3,890,834 to 3,891,241
ygdB
OKFHMN_20035: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 3,891,226 to 3,891,549
ppdC
OKFHMN_20040: recC - exodeoxyribonuclease V subunit gamma, at 3,891,562 to 3,894,930
recC
Position (kb)
3891
3892 Strain fitness (log2 ratio)
-2
-1
0
1 at 3890.338 kb on + strand, within pulJ at 3890.338 kb on + strand, within pulJ at 3890.338 kb on + strand, within pulJ at 3890.528 kb on + strand, within pulJ at 3890.528 kb on + strand, within pulJ at 3890.528 kb on + strand, within pulJ at 3890.562 kb on - strand, within pulJ at 3890.562 kb on - strand, within pulJ at 3890.699 kb on + strand, within pulJ at 3890.720 kb on - strand, within pulJ at 3890.762 kb on + strand, within pulJ at 3890.763 kb on - strand, within pulJ at 3890.811 kb on + strand at 3890.811 kb on + strand at 3890.812 kb on - strand at 3890.901 kb on + strand, within ygdB at 3891.049 kb on - strand, within ygdB at 3891.108 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.111 kb on + strand, within ygdB at 3891.112 kb on - strand, within ygdB at 3891.226 kb on - strand at 3891.336 kb on - strand, within ppdC at 3891.349 kb on - strand, within ppdC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,890,338 + pulJ OKFHMN_20025 0.11 -2.2 3,890,338 + pulJ OKFHMN_20025 0.11 +1.0 3,890,338 + pulJ OKFHMN_20025 0.11 +0.0 3,890,528 + pulJ OKFHMN_20025 0.45 +0.3 3,890,528 + pulJ OKFHMN_20025 0.45 -0.3 3,890,528 + pulJ OKFHMN_20025 0.45 +0.4 3,890,562 - pulJ OKFHMN_20025 0.51 +0.5 3,890,562 - pulJ OKFHMN_20025 0.51 +1.4 3,890,699 + pulJ OKFHMN_20025 0.75 +0.2 3,890,720 - pulJ OKFHMN_20025 0.79 -0.5 3,890,762 + pulJ OKFHMN_20025 0.87 -0.7 3,890,763 - pulJ OKFHMN_20025 0.87 -0.5 3,890,811 + -0.1 3,890,811 + -1.6 3,890,812 - -1.4 3,890,901 + ygdB OKFHMN_20030 0.16 -2.3 3,891,049 - ygdB OKFHMN_20030 0.53 +0.4 3,891,108 + ygdB OKFHMN_20030 0.67 -0.2 3,891,111 + ygdB OKFHMN_20030 0.68 +0.2 3,891,111 + ygdB OKFHMN_20030 0.68 +0.6 3,891,111 + ygdB OKFHMN_20030 0.68 -2.4 3,891,112 - ygdB OKFHMN_20030 0.68 +1.0 3,891,226 - +0.5 3,891,336 - ppdC OKFHMN_20035 0.34 -1.7 3,891,349 - ppdC OKFHMN_20035 0.38 -1.3
Or see this region's nucleotide sequence