Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19050

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybE and hypA overlap by 8 nucleotideshypA and hybG are separated by 12 nucleotideshybG and yghU are separated by 122 nucleotidesyghU and gss are separated by 204 nucleotides OKFHMN_19030: hybE - hydrogenase-2 assembly chaperone, at 3,695,243 to 3,695,731 hybE OKFHMN_19035: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,695,724 to 3,696,065 hypA OKFHMN_19040: hybG - hydrogenase maturation factor HybG, at 3,696,078 to 3,696,326 hybG OKFHMN_19050: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 3,696,449 to 3,697,315 yghU OKFHMN_19055: gss - bifunctional glutathionylspermidine amidase/synthase, at 3,697,520 to 3,699,379 gss Position (kb) 3696 3697 3698Strain fitness (log2 ratio) -3 -2 -1 0 1at 3695.555 kb on + strand, within hybEat 3695.556 kb on - strand, within hybEat 3695.908 kb on + strand, within hypAat 3695.909 kb on - strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.919 kb on + strand, within hypAat 3695.921 kb on + strand, within hypAat 3695.922 kb on - strand, within hypAat 3696.074 kb on - strandat 3696.083 kb on + strandat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.149 kb on - strand, within hybGat 3696.194 kb on + strand, within hybGat 3696.194 kb on + strand, within hybGat 3696.283 kb on + strand, within hybGat 3696.283 kb on + strand, within hybGat 3696.315 kb on - strandat 3696.341 kb on - strandat 3696.463 kb on + strandat 3696.609 kb on + strand, within yghUat 3696.609 kb on + strand, within yghUat 3696.609 kb on + strand, within yghUat 3696.610 kb on - strand, within yghUat 3696.702 kb on + strand, within yghUat 3696.703 kb on - strand, within yghUat 3696.724 kb on + strand, within yghUat 3696.823 kb on - strand, within yghUat 3696.988 kb on + strand, within yghUat 3697.076 kb on + strand, within yghUat 3697.077 kb on - strand, within yghUat 3697.077 kb on - strand, within yghUat 3697.092 kb on + strand, within yghUat 3697.092 kb on + strand, within yghUat 3697.135 kb on + strand, within yghUat 3697.135 kb on + strand, within yghUat 3697.182 kb on - strand, within yghUat 3697.300 kb on - strandat 3697.303 kb on + strandat 3697.303 kb on + strandat 3697.304 kb on - strandat 3697.353 kb on + strandat 3697.420 kb on + strandat 3697.421 kb on - strandat 3697.421 kb on - strandat 3697.421 kb on - strandat 3697.447 kb on - strandat 3697.463 kb on + strandat 3697.464 kb on - strandat 3697.495 kb on - strandat 3697.501 kb on + strandat 3697.502 kb on - strandat 3697.507 kb on + strandat 3697.514 kb on - strandat 3697.596 kb on - strandat 3697.596 kb on - strandat 3697.655 kb on - strandat 3697.658 kb on + strandat 3697.658 kb on + strandat 3697.673 kb on + strandat 3697.674 kb on - strandat 3697.675 kb on + strandat 3697.731 kb on - strand, within gssat 3697.801 kb on + strand, within gssat 3697.933 kb on - strand, within gssat 3698.039 kb on + strand, within gssat 3698.108 kb on + strand, within gssat 3698.109 kb on - strand, within gssat 3698.228 kb on + strand, within gssat 3698.228 kb on + strand, within gssat 3698.229 kb on - strand, within gssat 3698.274 kb on - strand, within gssat 3698.274 kb on - strand, within gssat 3698.276 kb on + strand, within gssat 3698.277 kb on - strand, within gss

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,695,555 + hybE OKFHMN_19030 0.64 -0.3
3,695,556 - hybE OKFHMN_19030 0.64 +1.2
3,695,908 + hypA OKFHMN_19035 0.54 -0.6
3,695,909 - hypA OKFHMN_19035 0.54 -0.9
3,695,912 + hypA OKFHMN_19035 0.55 -0.3
3,695,912 + hypA OKFHMN_19035 0.55 +0.1
3,695,919 + hypA OKFHMN_19035 0.57 -0.7
3,695,921 + hypA OKFHMN_19035 0.58 +0.9
3,695,922 - hypA OKFHMN_19035 0.58 +0.5
3,696,074 - +0.1
3,696,083 + +0.6
3,696,148 + hybG OKFHMN_19040 0.28 -3.5
3,696,148 + hybG OKFHMN_19040 0.28 +1.6
3,696,148 + hybG OKFHMN_19040 0.28 +0.1
3,696,148 + hybG OKFHMN_19040 0.28 +0.2
3,696,149 - hybG OKFHMN_19040 0.29 -0.7
3,696,194 + hybG OKFHMN_19040 0.47 -1.4
3,696,194 + hybG OKFHMN_19040 0.47 -0.0
3,696,283 + hybG OKFHMN_19040 0.82 +0.1
3,696,283 + hybG OKFHMN_19040 0.82 -1.6
3,696,315 - -0.3
3,696,341 - -1.0
3,696,463 + +0.1
3,696,609 + yghU OKFHMN_19050 0.18 -0.2
3,696,609 + yghU OKFHMN_19050 0.18 -0.7
3,696,609 + yghU OKFHMN_19050 0.18 +1.3
3,696,610 - yghU OKFHMN_19050 0.19 -0.2
3,696,702 + yghU OKFHMN_19050 0.29 +0.0
3,696,703 - yghU OKFHMN_19050 0.29 -0.2
3,696,724 + yghU OKFHMN_19050 0.32 -0.4
3,696,823 - yghU OKFHMN_19050 0.43 +0.6
3,696,988 + yghU OKFHMN_19050 0.62 +0.0
3,697,076 + yghU OKFHMN_19050 0.72 -1.4
3,697,077 - yghU OKFHMN_19050 0.72 -0.1
3,697,077 - yghU OKFHMN_19050 0.72 -1.6
3,697,092 + yghU OKFHMN_19050 0.74 -0.4
3,697,092 + yghU OKFHMN_19050 0.74 -0.2
3,697,135 + yghU OKFHMN_19050 0.79 +0.2
3,697,135 + yghU OKFHMN_19050 0.79 -2.0
3,697,182 - yghU OKFHMN_19050 0.85 +0.3
3,697,300 - +0.5
3,697,303 + +1.2
3,697,303 + -0.3
3,697,304 - -0.0
3,697,353 + -0.7
3,697,420 + -0.2
3,697,421 - -0.3
3,697,421 - +0.2
3,697,421 - +0.2
3,697,447 - -0.1
3,697,463 + -1.2
3,697,464 - +1.0
3,697,495 - +0.3
3,697,501 + -0.1
3,697,502 - -3.4
3,697,507 + +0.1
3,697,514 - -1.6
3,697,596 - +1.7
3,697,596 - -0.7
3,697,655 - +0.4
3,697,658 + -0.2
3,697,658 + +0.4
3,697,673 + +1.6
3,697,674 - -0.2
3,697,675 + -1.0
3,697,731 - gss OKFHMN_19055 0.11 +0.0
3,697,801 + gss OKFHMN_19055 0.15 -0.2
3,697,933 - gss OKFHMN_19055 0.22 -0.0
3,698,039 + gss OKFHMN_19055 0.28 +0.0
3,698,108 + gss OKFHMN_19055 0.32 +0.2
3,698,109 - gss OKFHMN_19055 0.32 -0.5
3,698,228 + gss OKFHMN_19055 0.38 +0.5
3,698,228 + gss OKFHMN_19055 0.38 -2.1
3,698,229 - gss OKFHMN_19055 0.38 -0.1
3,698,274 - gss OKFHMN_19055 0.41 +0.1
3,698,274 - gss OKFHMN_19055 0.41 -3.1
3,698,276 + gss OKFHMN_19055 0.41 -0.0
3,698,277 - gss OKFHMN_19055 0.41 +0.6

Or see this region's nucleotide sequence