Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19020

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybB and hybC overlap by 4 nucleotideshybC and hybD overlap by 1 nucleotideshybD and hybE overlap by 8 nucleotideshybE and hypA overlap by 8 nucleotides OKFHMN_19015: hybB - Ni/Fe-hydrogenase cytochrome b subunit, at 3,691,878 to 3,693,056 hybB OKFHMN_19020: hybC - hydrogenase 2 large subunit, at 3,693,053 to 3,694,756 hybC OKFHMN_19025: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,694,756 to 3,695,250 hybD OKFHMN_19030: hybE - hydrogenase-2 assembly chaperone, at 3,695,243 to 3,695,731 hybE OKFHMN_19035: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,695,724 to 3,696,065 hypA Position (kb) 3693 3694 3695Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3692.105 kb on - strand, within hybBat 3692.106 kb on + strand, within hybBat 3692.127 kb on + strand, within hybBat 3692.128 kb on - strand, within hybBat 3692.172 kb on + strand, within hybBat 3692.230 kb on - strand, within hybBat 3692.230 kb on - strand, within hybBat 3692.288 kb on + strand, within hybBat 3692.298 kb on + strand, within hybBat 3692.299 kb on - strand, within hybBat 3692.466 kb on + strand, within hybBat 3692.684 kb on + strand, within hybBat 3692.684 kb on + strand, within hybBat 3692.684 kb on + strand, within hybBat 3692.685 kb on - strand, within hybBat 3692.685 kb on - strand, within hybBat 3692.685 kb on - strand, within hybBat 3692.801 kb on + strand, within hybBat 3692.802 kb on - strand, within hybBat 3692.846 kb on - strand, within hybBat 3692.846 kb on - strand, within hybBat 3692.846 kb on - strand, within hybBat 3692.846 kb on - strand, within hybBat 3692.980 kb on + strandat 3693.001 kb on + strandat 3693.001 kb on + strandat 3693.029 kb on + strandat 3693.029 kb on + strandat 3693.067 kb on + strandat 3693.071 kb on - strandat 3693.102 kb on + strandat 3693.102 kb on + strandat 3693.103 kb on - strandat 3693.103 kb on - strandat 3693.229 kb on + strand, within hybCat 3693.240 kb on + strand, within hybCat 3693.241 kb on - strand, within hybCat 3693.244 kb on + strand, within hybCat 3693.244 kb on + strand, within hybCat 3693.245 kb on - strand, within hybCat 3693.245 kb on - strand, within hybCat 3693.245 kb on - strand, within hybCat 3693.245 kb on - strand, within hybCat 3693.247 kb on + strand, within hybCat 3693.320 kb on + strand, within hybCat 3693.321 kb on - strand, within hybCat 3693.321 kb on - strand, within hybCat 3693.378 kb on - strand, within hybCat 3693.623 kb on + strand, within hybCat 3693.624 kb on - strand, within hybCat 3693.776 kb on + strand, within hybCat 3693.777 kb on - strand, within hybCat 3693.777 kb on - strand, within hybCat 3693.824 kb on + strand, within hybCat 3693.825 kb on - strand, within hybCat 3693.960 kb on - strand, within hybCat 3694.016 kb on + strand, within hybCat 3694.058 kb on + strand, within hybCat 3694.059 kb on - strand, within hybCat 3694.059 kb on - strand, within hybCat 3694.094 kb on - strand, within hybCat 3694.109 kb on + strand, within hybCat 3694.198 kb on + strand, within hybCat 3694.199 kb on - strand, within hybCat 3694.199 kb on - strand, within hybCat 3694.379 kb on + strand, within hybCat 3694.492 kb on + strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.579 kb on + strand, within hybCat 3694.625 kb on - strandat 3694.646 kb on - strandat 3694.666 kb on + strandat 3694.667 kb on - strandat 3694.667 kb on - strandat 3694.755 kb on - strandat 3694.761 kb on + strandat 3694.858 kb on + strand, within hybDat 3694.858 kb on + strand, within hybDat 3694.859 kb on - strand, within hybDat 3695.257 kb on - strandat 3695.289 kb on + strandat 3695.289 kb on + strandat 3695.555 kb on + strand, within hybEat 3695.556 kb on - strand, within hybE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,692,105 - hybB OKFHMN_19015 0.19 -0.4
3,692,106 + hybB OKFHMN_19015 0.19 -0.4
3,692,127 + hybB OKFHMN_19015 0.21 -1.3
3,692,128 - hybB OKFHMN_19015 0.21 -1.4
3,692,172 + hybB OKFHMN_19015 0.25 -0.9
3,692,230 - hybB OKFHMN_19015 0.30 -2.0
3,692,230 - hybB OKFHMN_19015 0.30 +0.1
3,692,288 + hybB OKFHMN_19015 0.35 +0.6
3,692,298 + hybB OKFHMN_19015 0.36 +0.4
3,692,299 - hybB OKFHMN_19015 0.36 +1.1
3,692,466 + hybB OKFHMN_19015 0.50 -0.2
3,692,684 + hybB OKFHMN_19015 0.68 +0.0
3,692,684 + hybB OKFHMN_19015 0.68 +0.2
3,692,684 + hybB OKFHMN_19015 0.68 +0.5
3,692,685 - hybB OKFHMN_19015 0.68 -0.0
3,692,685 - hybB OKFHMN_19015 0.68 -0.0
3,692,685 - hybB OKFHMN_19015 0.68 -0.9
3,692,801 + hybB OKFHMN_19015 0.78 +0.1
3,692,802 - hybB OKFHMN_19015 0.78 -0.9
3,692,846 - hybB OKFHMN_19015 0.82 +0.2
3,692,846 - hybB OKFHMN_19015 0.82 +0.5
3,692,846 - hybB OKFHMN_19015 0.82 -0.9
3,692,846 - hybB OKFHMN_19015 0.82 +0.8
3,692,980 + +0.8
3,693,001 + +0.2
3,693,001 + -1.0
3,693,029 + +0.3
3,693,029 + -1.1
3,693,067 + +0.2
3,693,071 - -1.6
3,693,102 + -2.3
3,693,102 + +1.7
3,693,103 - -1.5
3,693,103 - -0.3
3,693,229 + hybC OKFHMN_19020 0.10 +0.7
3,693,240 + hybC OKFHMN_19020 0.11 -0.0
3,693,241 - hybC OKFHMN_19020 0.11 +2.3
3,693,244 + hybC OKFHMN_19020 0.11 +1.3
3,693,244 + hybC OKFHMN_19020 0.11 +1.2
3,693,245 - hybC OKFHMN_19020 0.11 -1.1
3,693,245 - hybC OKFHMN_19020 0.11 +0.5
3,693,245 - hybC OKFHMN_19020 0.11 -0.5
3,693,245 - hybC OKFHMN_19020 0.11 +0.3
3,693,247 + hybC OKFHMN_19020 0.11 +0.1
3,693,320 + hybC OKFHMN_19020 0.16 -0.2
3,693,321 - hybC OKFHMN_19020 0.16 -0.9
3,693,321 - hybC OKFHMN_19020 0.16 -0.1
3,693,378 - hybC OKFHMN_19020 0.19 +2.8
3,693,623 + hybC OKFHMN_19020 0.33 -1.0
3,693,624 - hybC OKFHMN_19020 0.34 +0.8
3,693,776 + hybC OKFHMN_19020 0.42 -1.1
3,693,777 - hybC OKFHMN_19020 0.42 +1.2
3,693,777 - hybC OKFHMN_19020 0.42 -0.2
3,693,824 + hybC OKFHMN_19020 0.45 -0.3
3,693,825 - hybC OKFHMN_19020 0.45 +0.2
3,693,960 - hybC OKFHMN_19020 0.53 -1.1
3,694,016 + hybC OKFHMN_19020 0.57 +0.6
3,694,058 + hybC OKFHMN_19020 0.59 +0.1
3,694,059 - hybC OKFHMN_19020 0.59 -1.3
3,694,059 - hybC OKFHMN_19020 0.59 -0.7
3,694,094 - hybC OKFHMN_19020 0.61 -1.3
3,694,109 + hybC OKFHMN_19020 0.62 +0.2
3,694,198 + hybC OKFHMN_19020 0.67 +0.8
3,694,199 - hybC OKFHMN_19020 0.67 -3.0
3,694,199 - hybC OKFHMN_19020 0.67 +0.5
3,694,379 + hybC OKFHMN_19020 0.78 +0.7
3,694,492 + hybC OKFHMN_19020 0.84 -0.2
3,694,539 - hybC OKFHMN_19020 0.87 +0.4
3,694,539 - hybC OKFHMN_19020 0.87 -0.1
3,694,539 - hybC OKFHMN_19020 0.87 -0.4
3,694,539 - hybC OKFHMN_19020 0.87 +0.0
3,694,539 - hybC OKFHMN_19020 0.87 -0.2
3,694,579 + hybC OKFHMN_19020 0.90 +0.3
3,694,625 - -1.0
3,694,646 - -1.7
3,694,666 + -0.5
3,694,667 - +0.2
3,694,667 - +0.2
3,694,755 - +0.3
3,694,761 + -0.9
3,694,858 + hybD OKFHMN_19025 0.21 +0.1
3,694,858 + hybD OKFHMN_19025 0.21 +0.1
3,694,859 - hybD OKFHMN_19025 0.21 +0.5
3,695,257 - -0.5
3,695,289 + +0.6
3,695,289 + -0.5
3,695,555 + hybE OKFHMN_19030 0.64 -0.3
3,695,556 - hybE OKFHMN_19030 0.64 +1.2

Or see this region's nucleotide sequence