Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudF and yqiB are separated by 0 nucleotides yqiB and cpdA are separated by 24 nucleotides cpdA and cpdA overlap by 1 nucleotides cpdA and OKFHMN_18830 are separated by 28 nucleotides
OKFHMN_18810: nudF - ADP-ribose diphosphatase, at 3,652,391 to 3,653,020
nudF
OKFHMN_18815: yqiB - DUF1249 domain-containing protein, at 3,653,021 to 3,653,443
yqiB
OKFHMN_18820: cpdA - 3',5'-cyclic-AMP phosphodiesterase, at 3,653,468 to 3,654,295
cpdA
OKFHMN_18825: cpdA - 3',5'-cyclic-AMP phosphodiesterase, at 3,654,295 to 3,654,876
cpdA
OKFHMN_18830: OKFHMN_18830 - DNA topoisomerase 4 subunit B, at 3,654,905 to 3,656,797
_18830
Position (kb)
3653
3654
3655 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3652.492 kb on + strand, within nudF at 3652.537 kb on - strand, within nudF at 3652.578 kb on + strand, within nudF at 3652.638 kb on - strand, within nudF at 3652.646 kb on - strand, within nudF at 3652.663 kb on - strand, within nudF at 3652.773 kb on + strand, within nudF at 3652.774 kb on - strand, within nudF at 3652.819 kb on + strand, within nudF at 3652.820 kb on - strand, within nudF at 3652.919 kb on + strand, within nudF at 3652.920 kb on - strand, within nudF at 3652.920 kb on - strand, within nudF at 3652.920 kb on - strand, within nudF at 3653.019 kb on - strand at 3653.019 kb on - strand at 3653.031 kb on - strand at 3653.128 kb on - strand, within yqiB at 3653.132 kb on + strand, within yqiB at 3653.132 kb on + strand, within yqiB at 3653.132 kb on + strand, within yqiB at 3653.184 kb on - strand, within yqiB at 3653.202 kb on + strand, within yqiB at 3653.202 kb on + strand, within yqiB at 3653.203 kb on - strand, within yqiB at 3653.229 kb on - strand, within yqiB at 3653.335 kb on + strand, within yqiB at 3653.338 kb on + strand, within yqiB at 3653.409 kb on + strand at 3653.409 kb on + strand at 3653.442 kb on - strand at 3653.527 kb on + strand at 3653.688 kb on - strand, within cpdA at 3653.771 kb on + strand, within cpdA at 3653.970 kb on + strand, within cpdA at 3653.983 kb on + strand, within cpdA at 3653.983 kb on + strand, within cpdA at 3654.181 kb on + strand, within cpdA at 3654.290 kb on + strand at 3654.300 kb on + strand at 3654.300 kb on + strand at 3654.310 kb on + strand at 3654.314 kb on + strand at 3654.486 kb on + strand, within cpdA at 3654.511 kb on + strand, within cpdA at 3654.511 kb on + strand, within cpdA at 3654.540 kb on + strand, within cpdA at 3654.598 kb on + strand, within cpdA at 3654.637 kb on + strand, within cpdA at 3654.637 kb on + strand, within cpdA at 3654.637 kb on + strand, within cpdA at 3654.638 kb on - strand, within cpdA at 3654.638 kb on - strand, within cpdA at 3654.657 kb on + strand, within cpdA at 3654.669 kb on + strand, within cpdA at 3654.769 kb on + strand, within cpdA at 3654.769 kb on + strand, within cpdA at 3654.794 kb on + strand, within cpdA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,652,492 + nudF OKFHMN_18810 0.16 +0.0 3,652,537 - nudF OKFHMN_18810 0.23 +0.3 3,652,578 + nudF OKFHMN_18810 0.30 -0.4 3,652,638 - nudF OKFHMN_18810 0.39 -0.7 3,652,646 - nudF OKFHMN_18810 0.40 -1.5 3,652,663 - nudF OKFHMN_18810 0.43 -0.6 3,652,773 + nudF OKFHMN_18810 0.61 +1.3 3,652,774 - nudF OKFHMN_18810 0.61 +2.2 3,652,819 + nudF OKFHMN_18810 0.68 -0.7 3,652,820 - nudF OKFHMN_18810 0.68 -0.2 3,652,919 + nudF OKFHMN_18810 0.84 +1.0 3,652,920 - nudF OKFHMN_18810 0.84 +0.2 3,652,920 - nudF OKFHMN_18810 0.84 +0.5 3,652,920 - nudF OKFHMN_18810 0.84 +0.5 3,653,019 - +0.8 3,653,019 - -0.4 3,653,031 - -0.4 3,653,128 - yqiB OKFHMN_18815 0.25 -0.2 3,653,132 + yqiB OKFHMN_18815 0.26 +0.2 3,653,132 + yqiB OKFHMN_18815 0.26 -0.1 3,653,132 + yqiB OKFHMN_18815 0.26 -1.0 3,653,184 - yqiB OKFHMN_18815 0.39 +0.0 3,653,202 + yqiB OKFHMN_18815 0.43 -0.5 3,653,202 + yqiB OKFHMN_18815 0.43 +0.2 3,653,203 - yqiB OKFHMN_18815 0.43 +1.5 3,653,229 - yqiB OKFHMN_18815 0.49 -0.6 3,653,335 + yqiB OKFHMN_18815 0.74 -0.0 3,653,338 + yqiB OKFHMN_18815 0.75 -2.6 3,653,409 + +0.2 3,653,409 + +0.0 3,653,442 - +0.6 3,653,527 + +1.4 3,653,688 - cpdA OKFHMN_18820 0.27 +0.7 3,653,771 + cpdA OKFHMN_18820 0.37 -0.6 3,653,970 + cpdA OKFHMN_18820 0.61 +0.8 3,653,983 + cpdA OKFHMN_18820 0.62 +0.0 3,653,983 + cpdA OKFHMN_18820 0.62 +0.2 3,654,181 + cpdA OKFHMN_18820 0.86 +0.6 3,654,290 + -0.1 3,654,300 + -0.1 3,654,300 + -0.8 3,654,310 + +1.0 3,654,314 + -0.1 3,654,486 + cpdA OKFHMN_18825 0.33 +0.9 3,654,511 + cpdA OKFHMN_18825 0.37 +0.2 3,654,511 + cpdA OKFHMN_18825 0.37 +1.5 3,654,540 + cpdA OKFHMN_18825 0.42 +0.3 3,654,598 + cpdA OKFHMN_18825 0.52 +1.4 3,654,637 + cpdA OKFHMN_18825 0.59 +0.2 3,654,637 + cpdA OKFHMN_18825 0.59 +0.6 3,654,637 + cpdA OKFHMN_18825 0.59 -1.3 3,654,638 - cpdA OKFHMN_18825 0.59 +0.4 3,654,638 - cpdA OKFHMN_18825 0.59 +0.4 3,654,657 + cpdA OKFHMN_18825 0.62 -1.4 3,654,669 + cpdA OKFHMN_18825 0.64 +0.4 3,654,769 + cpdA OKFHMN_18825 0.81 -0.2 3,654,769 + cpdA OKFHMN_18825 0.81 +0.2 3,654,794 + cpdA OKFHMN_18825 0.86 +0.7
Or see this region's nucleotide sequence