Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysG and nirC are separated by 18 nucleotides nirC and nirD are separated by 125 nucleotides nirD and nirB overlap by 4 nucleotides
OKFHMN_17180: cysG - siroheme synthase CysG, at 3,355,974 to 3,357,347
cysG
OKFHMN_17185: nirC - nitrite transporter NirC, at 3,357,366 to 3,358,172
nirC
OKFHMN_17190: nirD - nitrite reductase small subunit NirD, at 3,358,298 to 3,358,624
nirD
OKFHMN_17195: nirB - NADPH-nitrite reductase large subunit, at 3,358,621 to 3,361,164
nirB
Position (kb)
3358
3359 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3357.318 kb on + strand at 3357.319 kb on - strand at 3357.319 kb on - strand at 3357.330 kb on + strand at 3357.350 kb on - strand at 3357.364 kb on - strand at 3357.364 kb on - strand at 3357.364 kb on - strand at 3357.364 kb on - strand at 3357.428 kb on + strand at 3357.433 kb on + strand at 3357.434 kb on - strand at 3357.434 kb on - strand at 3357.434 kb on - strand at 3357.456 kb on + strand, within nirC at 3357.469 kb on - strand, within nirC at 3357.473 kb on + strand, within nirC at 3357.473 kb on + strand, within nirC at 3357.474 kb on - strand, within nirC at 3357.474 kb on - strand, within nirC at 3357.573 kb on - strand, within nirC at 3357.693 kb on - strand, within nirC at 3357.717 kb on + strand, within nirC at 3357.771 kb on - strand, within nirC at 3357.803 kb on + strand, within nirC at 3357.803 kb on + strand, within nirC at 3357.803 kb on + strand, within nirC at 3357.803 kb on + strand, within nirC at 3357.804 kb on - strand, within nirC at 3357.804 kb on - strand, within nirC at 3357.804 kb on - strand, within nirC at 3357.805 kb on + strand, within nirC at 3357.806 kb on - strand, within nirC at 3357.806 kb on - strand, within nirC at 3357.806 kb on - strand, within nirC at 3358.063 kb on + strand, within nirC at 3358.064 kb on - strand, within nirC at 3358.152 kb on - strand at 3358.155 kb on - strand at 3358.248 kb on + strand at 3358.341 kb on + strand, within nirD at 3358.342 kb on - strand, within nirD at 3358.556 kb on + strand, within nirD at 3358.557 kb on - strand, within nirD at 3358.645 kb on + strand at 3358.646 kb on - strand at 3358.654 kb on - strand at 3358.767 kb on + strand at 3358.768 kb on - strand at 3358.938 kb on + strand, within nirB at 3359.051 kb on + strand, within nirB at 3359.051 kb on + strand, within nirB at 3359.052 kb on - strand, within nirB at 3359.079 kb on + strand, within nirB at 3359.083 kb on - strand, within nirB at 3359.385 kb on - strand, within nirB at 3359.406 kb on + strand, within nirB at 3359.406 kb on + strand, within nirB at 3359.413 kb on + strand, within nirB at 3359.471 kb on + strand, within nirB at 3359.472 kb on - strand, within nirB at 3359.494 kb on - strand, within nirB at 3359.530 kb on - strand, within nirB at 3359.549 kb on + strand, within nirB at 3359.609 kb on + strand, within nirB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,357,318 + +0.2 3,357,319 - -0.5 3,357,319 - -0.7 3,357,330 + +0.6 3,357,350 - -0.2 3,357,364 - +0.8 3,357,364 - -1.3 3,357,364 - -0.5 3,357,364 - +0.1 3,357,428 + +2.7 3,357,433 + -1.8 3,357,434 - +0.3 3,357,434 - -0.6 3,357,434 - -0.2 3,357,456 + nirC OKFHMN_17185 0.11 +0.1 3,357,469 - nirC OKFHMN_17185 0.13 -0.6 3,357,473 + nirC OKFHMN_17185 0.13 -0.8 3,357,473 + nirC OKFHMN_17185 0.13 +0.4 3,357,474 - nirC OKFHMN_17185 0.13 -0.7 3,357,474 - nirC OKFHMN_17185 0.13 -3.9 3,357,573 - nirC OKFHMN_17185 0.26 +0.8 3,357,693 - nirC OKFHMN_17185 0.41 +0.7 3,357,717 + nirC OKFHMN_17185 0.43 +0.5 3,357,771 - nirC OKFHMN_17185 0.50 +0.8 3,357,803 + nirC OKFHMN_17185 0.54 +0.4 3,357,803 + nirC OKFHMN_17185 0.54 +0.6 3,357,803 + nirC OKFHMN_17185 0.54 -0.3 3,357,803 + nirC OKFHMN_17185 0.54 -0.2 3,357,804 - nirC OKFHMN_17185 0.54 -0.8 3,357,804 - nirC OKFHMN_17185 0.54 +0.5 3,357,804 - nirC OKFHMN_17185 0.54 +0.1 3,357,805 + nirC OKFHMN_17185 0.54 +0.7 3,357,806 - nirC OKFHMN_17185 0.55 -1.2 3,357,806 - nirC OKFHMN_17185 0.55 -0.6 3,357,806 - nirC OKFHMN_17185 0.55 +0.6 3,358,063 + nirC OKFHMN_17185 0.86 -1.0 3,358,064 - nirC OKFHMN_17185 0.86 +0.8 3,358,152 - +0.2 3,358,155 - +0.2 3,358,248 + +0.2 3,358,341 + nirD OKFHMN_17190 0.13 +0.9 3,358,342 - nirD OKFHMN_17190 0.13 +0.4 3,358,556 + nirD OKFHMN_17190 0.79 -0.8 3,358,557 - nirD OKFHMN_17190 0.79 -0.5 3,358,645 + +1.0 3,358,646 - +0.4 3,358,654 - +0.9 3,358,767 + -1.6 3,358,768 - +0.3 3,358,938 + nirB OKFHMN_17195 0.12 -0.2 3,359,051 + nirB OKFHMN_17195 0.17 -0.2 3,359,051 + nirB OKFHMN_17195 0.17 +0.3 3,359,052 - nirB OKFHMN_17195 0.17 -0.1 3,359,079 + nirB OKFHMN_17195 0.18 -0.4 3,359,083 - nirB OKFHMN_17195 0.18 +0.4 3,359,385 - nirB OKFHMN_17195 0.30 +0.2 3,359,406 + nirB OKFHMN_17195 0.31 -0.0 3,359,406 + nirB OKFHMN_17195 0.31 +0.8 3,359,413 + nirB OKFHMN_17195 0.31 +0.7 3,359,471 + nirB OKFHMN_17195 0.33 +1.2 3,359,472 - nirB OKFHMN_17195 0.33 +0.2 3,359,494 - nirB OKFHMN_17195 0.34 -0.7 3,359,530 - nirB OKFHMN_17195 0.36 -3.6 3,359,549 + nirB OKFHMN_17195 0.36 +0.3 3,359,609 + nirB OKFHMN_17195 0.39 +0.8
Or see this region's nucleotide sequence