Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhgF and greB are separated by 96 nucleotides greB and ompR_G63V are separated by 227 nucleotides ompR_G63V and envZ overlap by 4 nucleotides
OKFHMN_17025: yhgF - Protein YhgF, at 3,323,181 to 3,325,502
yhgF
OKFHMN_17030: greB - transcription elongation factor GreB, at 3,325,599 to 3,326,075
greB
OKFHMN_17035: ompR_G63V - two-component system response regulator OmpR, at 3,326,303 to 3,327,022
_G63V
OKFHMN_17040: envZ - two-component system sensor histidine kinase EnvZ, at 3,327,019 to 3,328,371
envZ
Position (kb)
3325
3326
3327 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3324.745 kb on + strand, within yhgF at 3324.745 kb on + strand, within yhgF at 3324.745 kb on + strand, within yhgF at 3324.746 kb on - strand, within yhgF at 3324.746 kb on - strand, within yhgF at 3324.801 kb on + strand, within yhgF at 3324.802 kb on - strand, within yhgF at 3324.802 kb on - strand, within yhgF at 3324.881 kb on - strand, within yhgF at 3324.955 kb on + strand, within yhgF at 3325.010 kb on + strand, within yhgF at 3325.011 kb on - strand, within yhgF at 3325.012 kb on + strand, within yhgF at 3325.013 kb on - strand, within yhgF at 3325.013 kb on - strand, within yhgF at 3325.067 kb on + strand, within yhgF at 3325.068 kb on - strand, within yhgF at 3325.068 kb on - strand, within yhgF at 3325.068 kb on - strand, within yhgF at 3325.068 kb on - strand, within yhgF at 3325.069 kb on + strand, within yhgF at 3325.176 kb on - strand, within yhgF at 3325.176 kb on - strand, within yhgF at 3325.192 kb on + strand, within yhgF at 3325.192 kb on + strand, within yhgF at 3325.219 kb on - strand, within yhgF at 3325.394 kb on + strand at 3325.394 kb on + strand at 3325.394 kb on + strand at 3325.395 kb on - strand at 3325.476 kb on - strand at 3325.540 kb on + strand at 3325.540 kb on + strand at 3325.541 kb on - strand at 3325.541 kb on - strand at 3325.544 kb on + strand at 3325.544 kb on + strand at 3325.545 kb on - strand at 3325.545 kb on - strand at 3325.545 kb on - strand at 3325.545 kb on - strand at 3325.587 kb on + strand at 3325.612 kb on + strand at 3325.612 kb on + strand at 3325.626 kb on - strand at 3325.631 kb on - strand at 3325.655 kb on + strand, within greB at 3325.727 kb on - strand, within greB at 3325.753 kb on + strand, within greB at 3325.846 kb on + strand, within greB at 3325.847 kb on - strand, within greB at 3325.847 kb on - strand, within greB at 3325.878 kb on - strand, within greB at 3325.878 kb on - strand, within greB at 3325.886 kb on - strand, within greB at 3325.922 kb on + strand, within greB at 3325.922 kb on + strand, within greB at 3325.926 kb on - strand, within greB at 3325.927 kb on + strand, within greB at 3325.927 kb on + strand, within greB at 3325.927 kb on + strand, within greB at 3325.928 kb on - strand, within greB at 3325.933 kb on + strand, within greB at 3326.017 kb on + strand, within greB at 3326.017 kb on + strand, within greB at 3326.021 kb on + strand, within greB at 3326.022 kb on - strand, within greB at 3326.022 kb on - strand, within greB at 3326.022 kb on - strand, within greB at 3326.102 kb on + strand at 3326.118 kb on + strand at 3326.118 kb on + strand at 3326.119 kb on - strand at 3326.120 kb on + strand at 3326.120 kb on + strand at 3326.132 kb on - strand at 3326.174 kb on + strand at 3326.174 kb on + strand at 3326.175 kb on - strand at 3326.175 kb on - strand at 3326.175 kb on - strand at 3326.186 kb on + strand at 3326.189 kb on + strand at 3326.230 kb on - strand at 3326.262 kb on - strand at 3326.269 kb on + strand at 3326.269 kb on + strand at 3326.316 kb on - strand at 3326.369 kb on + strand at 3326.369 kb on + strand at 3326.370 kb on - strand at 3326.455 kb on + strand, within ompR_G63V at 3326.455 kb on + strand, within ompR_G63V at 3326.455 kb on + strand, within ompR_G63V at 3326.456 kb on - strand, within ompR_G63V at 3326.456 kb on - strand, within ompR_G63V at 3326.461 kb on - strand, within ompR_G63V at 3326.470 kb on - strand, within ompR_G63V at 3326.475 kb on + strand, within ompR_G63V at 3326.476 kb on - strand, within ompR_G63V at 3326.576 kb on - strand, within ompR_G63V at 3326.990 kb on + strand at 3326.990 kb on + strand at 3327.061 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,324,745 + yhgF OKFHMN_17025 0.67 -0.7 3,324,745 + yhgF OKFHMN_17025 0.67 -0.4 3,324,745 + yhgF OKFHMN_17025 0.67 +0.9 3,324,746 - yhgF OKFHMN_17025 0.67 -1.5 3,324,746 - yhgF OKFHMN_17025 0.67 -0.7 3,324,801 + yhgF OKFHMN_17025 0.70 -0.4 3,324,802 - yhgF OKFHMN_17025 0.70 +0.7 3,324,802 - yhgF OKFHMN_17025 0.70 +1.0 3,324,881 - yhgF OKFHMN_17025 0.73 -0.0 3,324,955 + yhgF OKFHMN_17025 0.76 +0.4 3,325,010 + yhgF OKFHMN_17025 0.79 -0.1 3,325,011 - yhgF OKFHMN_17025 0.79 -0.0 3,325,012 + yhgF OKFHMN_17025 0.79 +1.2 3,325,013 - yhgF OKFHMN_17025 0.79 -0.3 3,325,013 - yhgF OKFHMN_17025 0.79 -0.4 3,325,067 + yhgF OKFHMN_17025 0.81 -0.4 3,325,068 - yhgF OKFHMN_17025 0.81 -1.1 3,325,068 - yhgF OKFHMN_17025 0.81 +0.2 3,325,068 - yhgF OKFHMN_17025 0.81 +0.4 3,325,068 - yhgF OKFHMN_17025 0.81 -0.3 3,325,069 + yhgF OKFHMN_17025 0.81 +0.3 3,325,176 - yhgF OKFHMN_17025 0.86 +0.3 3,325,176 - yhgF OKFHMN_17025 0.86 -0.1 3,325,192 + yhgF OKFHMN_17025 0.87 -1.9 3,325,192 + yhgF OKFHMN_17025 0.87 +0.1 3,325,219 - yhgF OKFHMN_17025 0.88 -0.1 3,325,394 + +0.2 3,325,394 + +0.4 3,325,394 + +0.2 3,325,395 - +0.4 3,325,476 - -0.1 3,325,540 + +0.4 3,325,540 + +1.0 3,325,541 - +0.1 3,325,541 - -0.2 3,325,544 + -0.7 3,325,544 + +0.2 3,325,545 - +0.2 3,325,545 - +0.8 3,325,545 - +0.0 3,325,545 - +0.5 3,325,587 + -2.3 3,325,612 + -1.0 3,325,612 + -2.6 3,325,626 - -0.7 3,325,631 - +1.2 3,325,655 + greB OKFHMN_17030 0.12 -0.4 3,325,727 - greB OKFHMN_17030 0.27 -0.1 3,325,753 + greB OKFHMN_17030 0.32 +0.5 3,325,846 + greB OKFHMN_17030 0.52 +0.6 3,325,847 - greB OKFHMN_17030 0.52 -1.5 3,325,847 - greB OKFHMN_17030 0.52 +0.4 3,325,878 - greB OKFHMN_17030 0.58 +0.3 3,325,878 - greB OKFHMN_17030 0.58 -0.1 3,325,886 - greB OKFHMN_17030 0.60 -0.1 3,325,922 + greB OKFHMN_17030 0.68 -0.0 3,325,922 + greB OKFHMN_17030 0.68 +0.4 3,325,926 - greB OKFHMN_17030 0.69 -0.2 3,325,927 + greB OKFHMN_17030 0.69 +0.2 3,325,927 + greB OKFHMN_17030 0.69 -0.1 3,325,927 + greB OKFHMN_17030 0.69 +0.0 3,325,928 - greB OKFHMN_17030 0.69 +1.3 3,325,933 + greB OKFHMN_17030 0.70 -0.0 3,326,017 + greB OKFHMN_17030 0.88 -0.8 3,326,017 + greB OKFHMN_17030 0.88 +3.9 3,326,021 + greB OKFHMN_17030 0.88 -1.5 3,326,022 - greB OKFHMN_17030 0.89 +0.8 3,326,022 - greB OKFHMN_17030 0.89 +0.6 3,326,022 - greB OKFHMN_17030 0.89 -1.0 3,326,102 + -0.7 3,326,118 + -0.1 3,326,118 + -2.6 3,326,119 - +0.3 3,326,120 + -0.2 3,326,120 + +1.5 3,326,132 - +0.7 3,326,174 + +0.1 3,326,174 + +0.4 3,326,175 - -1.2 3,326,175 - -0.3 3,326,175 - -2.4 3,326,186 + -0.0 3,326,189 + -0.2 3,326,230 - +0.4 3,326,262 - +0.4 3,326,269 + +0.1 3,326,269 + -0.6 3,326,316 - -0.1 3,326,369 + +1.0 3,326,369 + +2.1 3,326,370 - +2.1 3,326,455 + ompR_G63V OKFHMN_17035 0.21 +2.4 3,326,455 + ompR_G63V OKFHMN_17035 0.21 +1.8 3,326,455 + ompR_G63V OKFHMN_17035 0.21 -0.5 3,326,456 - ompR_G63V OKFHMN_17035 0.21 +0.9 3,326,456 - ompR_G63V OKFHMN_17035 0.21 +2.4 3,326,461 - ompR_G63V OKFHMN_17035 0.22 +1.7 3,326,470 - ompR_G63V OKFHMN_17035 0.23 +1.3 3,326,475 + ompR_G63V OKFHMN_17035 0.24 +1.6 3,326,476 - ompR_G63V OKFHMN_17035 0.24 +2.0 3,326,576 - ompR_G63V OKFHMN_17035 0.38 +0.0 3,326,990 + +0.3 3,326,990 + +2.0 3,327,061 - +1.7
Or see this region's nucleotide sequence