Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yiaG and yiaF are separated by 433 nucleotides yiaF and ghrB are separated by 49 nucleotides
OKFHMN_16010: yiaG - HTH-type transcriptional regulator, at 3,107,731 to 3,108,021
yiaG
OKFHMN_16015: yiaF - Uncharacterized protein YiaF, at 3,108,455 to 3,109,165
yiaF
OKFHMN_16020: ghrB - glyoxylate/hydroxypyruvate reductase GhrB, at 3,109,215 to 3,110,189
ghrB
Position (kb)
3108
3109
3110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3107.455 kb on + strand at 3107.456 kb on - strand at 3107.458 kb on + strand at 3107.458 kb on + strand at 3107.459 kb on - strand at 3107.467 kb on + strand at 3107.468 kb on - strand at 3107.553 kb on - strand at 3107.578 kb on - strand at 3107.620 kb on + strand at 3107.660 kb on + strand at 3107.670 kb on + strand at 3107.725 kb on - strand at 3107.748 kb on + strand at 3107.749 kb on - strand at 3107.849 kb on - strand, within yiaG at 3107.881 kb on + strand, within yiaG at 3107.900 kb on - strand, within yiaG at 3108.012 kb on + strand at 3108.012 kb on + strand at 3108.012 kb on + strand at 3108.074 kb on - strand at 3108.193 kb on + strand at 3108.193 kb on + strand at 3108.193 kb on + strand at 3108.193 kb on + strand at 3108.194 kb on - strand at 3108.195 kb on + strand at 3108.196 kb on - strand at 3108.219 kb on - strand at 3108.358 kb on + strand at 3108.359 kb on - strand at 3108.391 kb on - strand at 3108.513 kb on + strand at 3108.513 kb on + strand at 3108.513 kb on + strand at 3108.514 kb on - strand at 3108.514 kb on - strand at 3108.514 kb on - strand at 3108.518 kb on - strand at 3108.518 kb on - strand at 3108.538 kb on + strand, within yiaF at 3108.556 kb on + strand, within yiaF at 3108.557 kb on - strand, within yiaF at 3108.583 kb on + strand, within yiaF at 3108.610 kb on - strand, within yiaF at 3108.730 kb on - strand, within yiaF at 3108.764 kb on + strand, within yiaF at 3108.765 kb on - strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.004 kb on + strand, within yiaF at 3109.005 kb on - strand, within yiaF at 3109.038 kb on + strand, within yiaF at 3109.038 kb on + strand, within yiaF at 3109.082 kb on + strand, within yiaF at 3109.082 kb on + strand, within yiaF at 3109.083 kb on - strand, within yiaF at 3109.083 kb on - strand, within yiaF at 3109.084 kb on + strand, within yiaF at 3109.084 kb on + strand, within yiaF at 3109.141 kb on + strand at 3109.151 kb on - strand at 3109.163 kb on + strand at 3109.197 kb on - strand at 3109.217 kb on - strand at 3109.381 kb on - strand, within ghrB at 3109.491 kb on - strand, within ghrB at 3109.596 kb on + strand, within ghrB at 3109.597 kb on - strand, within ghrB at 3109.649 kb on + strand, within ghrB at 3109.669 kb on + strand, within ghrB at 3109.670 kb on - strand, within ghrB at 3109.893 kb on + strand, within ghrB at 3109.961 kb on - strand, within ghrB at 3110.146 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 3,107,455 + -1.4 3,107,456 - -0.3 3,107,458 + -0.6 3,107,458 + -2.9 3,107,459 - +0.5 3,107,467 + +2.6 3,107,468 - +0.8 3,107,553 - -0.2 3,107,578 - +1.4 3,107,620 + -0.7 3,107,660 + +0.1 3,107,670 + -0.2 3,107,725 - -0.4 3,107,748 + -0.9 3,107,749 - +0.3 3,107,849 - yiaG OKFHMN_16010 0.41 +0.1 3,107,881 + yiaG OKFHMN_16010 0.52 -3.3 3,107,900 - yiaG OKFHMN_16010 0.58 +0.0 3,108,012 + +0.5 3,108,012 + -0.4 3,108,012 + -0.2 3,108,074 - -0.6 3,108,193 + +0.3 3,108,193 + -0.5 3,108,193 + -0.2 3,108,193 + +0.3 3,108,194 - -0.4 3,108,195 + +2.1 3,108,196 - -1.8 3,108,219 - +0.3 3,108,358 + -0.3 3,108,359 - +0.5 3,108,391 - -0.3 3,108,513 + +0.3 3,108,513 + -0.2 3,108,513 + -0.4 3,108,514 - +2.0 3,108,514 - -0.4 3,108,514 - -1.5 3,108,518 - +0.1 3,108,518 - -3.0 3,108,538 + yiaF OKFHMN_16015 0.12 +1.1 3,108,556 + yiaF OKFHMN_16015 0.14 +0.2 3,108,557 - yiaF OKFHMN_16015 0.14 +1.2 3,108,583 + yiaF OKFHMN_16015 0.18 +0.3 3,108,610 - yiaF OKFHMN_16015 0.22 +0.9 3,108,730 - yiaF OKFHMN_16015 0.39 +0.6 3,108,764 + yiaF OKFHMN_16015 0.43 +0.0 3,108,765 - yiaF OKFHMN_16015 0.44 -1.0 3,109,004 + yiaF OKFHMN_16015 0.77 -0.1 3,109,004 + yiaF OKFHMN_16015 0.77 -0.2 3,109,004 + yiaF OKFHMN_16015 0.77 -0.5 3,109,004 + yiaF OKFHMN_16015 0.77 -0.3 3,109,004 + yiaF OKFHMN_16015 0.77 +0.2 3,109,004 + yiaF OKFHMN_16015 0.77 +0.6 3,109,005 - yiaF OKFHMN_16015 0.77 -0.5 3,109,038 + yiaF OKFHMN_16015 0.82 +0.6 3,109,038 + yiaF OKFHMN_16015 0.82 -0.2 3,109,082 + yiaF OKFHMN_16015 0.88 -0.2 3,109,082 + yiaF OKFHMN_16015 0.88 +0.3 3,109,083 - yiaF OKFHMN_16015 0.88 +1.0 3,109,083 - yiaF OKFHMN_16015 0.88 -1.9 3,109,084 + yiaF OKFHMN_16015 0.88 +0.3 3,109,084 + yiaF OKFHMN_16015 0.88 +0.7 3,109,141 + -1.5 3,109,151 - +0.3 3,109,163 + +0.4 3,109,197 - +0.5 3,109,217 - -1.0 3,109,381 - ghrB OKFHMN_16020 0.17 -3.3 3,109,491 - ghrB OKFHMN_16020 0.28 -0.3 3,109,596 + ghrB OKFHMN_16020 0.39 -1.6 3,109,597 - ghrB OKFHMN_16020 0.39 -0.3 3,109,649 + ghrB OKFHMN_16020 0.45 +0.5 3,109,669 + ghrB OKFHMN_16020 0.47 -1.4 3,109,670 - ghrB OKFHMN_16020 0.47 +0.7 3,109,893 + ghrB OKFHMN_16020 0.70 +0.6 3,109,961 - ghrB OKFHMN_16020 0.77 +0.5 3,110,146 - -0.4
Or see this region's nucleotide sequence