Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15320

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotidesgrlA and cesD are separated by 383 nucleotidescesD and escC are separated by 13 nucleotides OKFHMN_15310: etgA - type III secretion system LEE muramidase EtgA, at 2,959,819 to 2,960,277 etgA OKFHMN_15315: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 2,960,473 to 2,960,844 grlR OKFHMN_15320: grlA - type III secretion system LEE transcriptional regulator GrlA, at 2,960,899 to 2,961,312 grlA OKFHMN_15325: cesD - SycD/LcrH family type III secretion system chaperone CesD, at 2,961,696 to 2,962,151 cesD OKFHMN_15330: escC - type III secretion system LEE outer membrane ring protein EscC, at 2,962,165 to 2,963,703 escC Position (kb) 2960 2961 2962Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2960.193 kb on + strand, within etgAat 2960.232 kb on - strandat 2960.422 kb on - strandat 2960.539 kb on - strand, within grlRat 2960.624 kb on + strand, within grlRat 2960.690 kb on + strand, within grlRat 2960.806 kb on - strand, within grlRat 2960.974 kb on - strand, within grlAat 2961.253 kb on - strand, within grlAat 2961.271 kb on - strandat 2961.468 kb on + strandat 2961.484 kb on + strandat 2961.726 kb on - strandat 2961.761 kb on + strand, within cesDat 2961.823 kb on + strand, within cesDat 2962.024 kb on + strand, within cesDat 2962.025 kb on - strand, within cesDat 2962.032 kb on - strand, within cesDat 2962.032 kb on - strand, within cesD

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,960,193 + etgA OKFHMN_15310 0.81 -3.3
2,960,232 - +1.5
2,960,422 - +1.1
2,960,539 - grlR OKFHMN_15315 0.18 -0.6
2,960,624 + grlR OKFHMN_15315 0.41 -1.1
2,960,690 + grlR OKFHMN_15315 0.58 -0.6
2,960,806 - grlR OKFHMN_15315 0.90 +1.3
2,960,974 - grlA OKFHMN_15320 0.18 -0.1
2,961,253 - grlA OKFHMN_15320 0.86 +0.1
2,961,271 - +2.1
2,961,468 + -0.1
2,961,484 + -0.9
2,961,726 - +0.3
2,961,761 + cesD OKFHMN_15325 0.14 -2.7
2,961,823 + cesD OKFHMN_15325 0.28 -1.3
2,962,024 + cesD OKFHMN_15325 0.72 +0.6
2,962,025 - cesD OKFHMN_15325 0.72 +0.4
2,962,032 - cesD OKFHMN_15325 0.74 +2.8
2,962,032 - cesD OKFHMN_15325 0.74 -1.4

Or see this region's nucleotide sequence