Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15285

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescE and cesAB are separated by 12 nucleotidescesAB and escK overlap by 4 nucleotidesescK and escL overlap by 14 nucleotidesescL and escR are separated by 4 nucleotidesescR and escS overlap by 1 nucleotidesescS and escT overlap by 1 nucleotides OKFHMN_15270: escE - chaperone for EscF, at 2,955,308 to 2,955,526 escE OKFHMN_15275: cesAB - chaperone for EspA and EspB, at 2,955,539 to 2,955,853 cesAB OKFHMN_15280: escK - type III secretion system sorting platform component, at 2,955,850 to 2,956,449 escK OKFHMN_15285: escL - negative regulator EscL, stator protein, at 2,956,436 to 2,957,089 escL OKFHMN_15290: escR - Type III secretion systemminor export apparatus protein EscR, at 2,957,094 to 2,957,747 escR OKFHMN_15295: escS - type III secretion system LEE export apparatus protein EscS, at 2,957,747 to 2,958,016 escS OKFHMN_15300: escT - type III secretion system LEE export apparatus protein EscT, at 2,958,016 to 2,958,792 escT Position (kb) 2956 2957 2958Strain fitness (log2 ratio) -3 -2 -1 0 1at 2955.757 kb on + strand, within cesABat 2955.838 kb on + strandat 2955.971 kb on + strand, within escKat 2956.867 kb on + strand, within escLat 2956.954 kb on + strand, within escLat 2957.846 kb on - strand, within escSat 2957.846 kb on - strand, within escSat 2957.985 kb on + strand, within escSat 2958.026 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,955,757 + cesAB OKFHMN_15275 0.69 +0.5
2,955,838 + -0.2
2,955,971 + escK OKFHMN_15280 0.20 -0.4
2,956,867 + escL OKFHMN_15285 0.66 -0.9
2,956,954 + escL OKFHMN_15285 0.79 -1.0
2,957,846 - escS OKFHMN_15295 0.37 -1.9
2,957,846 - escS OKFHMN_15295 0.37 +0.4
2,957,985 + escS OKFHMN_15295 0.88 +0.2
2,958,026 + -3.2

Or see this region's nucleotide sequence