Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15010

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnpA and rpmH are separated by 49 nucleotidesrpmH and ysdD are separated by 229 nucleotidesysdD and dnaA are separated by 296 nucleotidesdnaA and dnaN are separated by 4 nucleotides OKFHMN_14995: rnpA - ribonuclease P protein component, at 2,903,994 to 2,904,320 rnpA OKFHMN_15000: rpmH - 50S ribosomal protein L34, at 2,904,370 to 2,904,510 rpmH OKFHMN_15005: ysdD - Protein YsdD, at 2,904,740 to 2,904,820 ysdD OKFHMN_15010: dnaA - chromosomal replication initiator protein DnaA, at 2,905,117 to 2,906,520 dnaA OKFHMN_15020: dnaN - DNA polymerase III subunit beta, at 2,906,525 to 2,907,625 dnaN Position (kb) 2905 2906 2907Strain fitness (log2 ratio) -1 0 1at 2904.653 kb on + strandat 2904.693 kb on + strandat 2904.741 kb on + strandat 2904.741 kb on + strandat 2904.742 kb on - strandat 2904.743 kb on + strandat 2904.744 kb on - strandat 2904.797 kb on - strand, within ysdDat 2904.873 kb on + strandat 2904.873 kb on + strandat 2904.874 kb on - strandat 2905.001 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,904,653 + +1.3
2,904,693 + +0.2
2,904,741 + +0.4
2,904,741 + +0.6
2,904,742 - -0.3
2,904,743 + +0.1
2,904,744 - +0.4
2,904,797 - ysdD OKFHMN_15005 0.70 -0.1
2,904,873 + -0.7
2,904,873 + +0.5
2,904,874 - +0.2
2,905,001 + +0.2

Or see this region's nucleotide sequence