Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yifB and maoP are separated by 24 nucleotides maoP and hdfR are separated by 118 nucleotides hdfR and Trp_trna are separated by 95 nucleotides Trp_trna and Asp_trna are separated by 8 nucleotides Asp_trna and 5S_rrna are separated by 54 nucleotides 5S_rrna and 23S_rrna are separated by 94 nucleotides
OKFHMN_14665: yifB - YifB family Mg chelatase-like AAA ATPase, at 2,831,666 to 2,833,186
yifB
OKFHMN_14670: maoP - macrodomain Ori organization protein MaoP, at 2,833,211 to 2,833,549
maoP
OKFHMN_14675: hdfR - HTH-type transcriptional regulator HdfR, at 2,833,668 to 2,834,507
hdfR
OKFHMN_14680: Trp_trna - tRNA-Trp(cca), at 2,834,603 to 2,834,678
_trna
OKFHMN_14685: Asp_trna - tRNA-Asp(gtc), at 2,834,687 to 2,834,763
_trna
OKFHMN_14690: 5S_rrna - 5S ribosomal RNA, at 2,834,818 to 2,834,933
_rrna
OKFHMN_14695: 23S_rrna - 23S ribosomal RNA, at 2,835,028 to 2,837,932
_rrna
Position (kb)
2833
2834
2835 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.759 kb on + strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.760 kb on - strand, within yifB at 2832.848 kb on - strand, within yifB at 2832.884 kb on - strand, within yifB at 2832.894 kb on + strand, within yifB at 2832.894 kb on + strand, within yifB at 2832.894 kb on + strand, within yifB at 2832.895 kb on - strand, within yifB at 2832.952 kb on + strand, within yifB at 2833.084 kb on + strand at 2833.084 kb on + strand at 2833.132 kb on + strand at 2833.132 kb on + strand at 2833.132 kb on + strand at 2833.133 kb on - strand at 2833.136 kb on + strand at 2833.176 kb on + strand at 2833.176 kb on + strand at 2833.177 kb on - strand at 2833.212 kb on + strand at 2833.212 kb on + strand at 2833.231 kb on - strand at 2833.307 kb on - strand, within maoP at 2833.308 kb on + strand, within maoP at 2833.308 kb on + strand, within maoP at 2833.309 kb on - strand, within maoP at 2833.309 kb on - strand, within maoP at 2833.309 kb on - strand, within maoP at 2833.361 kb on + strand, within maoP at 2833.362 kb on - strand, within maoP at 2833.368 kb on - strand, within maoP at 2833.431 kb on + strand, within maoP at 2833.431 kb on + strand, within maoP at 2833.431 kb on + strand, within maoP at 2833.432 kb on - strand, within maoP at 2833.507 kb on + strand, within maoP at 2833.507 kb on + strand, within maoP at 2833.508 kb on - strand, within maoP at 2833.508 kb on - strand, within maoP at 2833.562 kb on + strand at 2833.563 kb on - strand at 2833.583 kb on + strand at 2833.599 kb on - strand at 2833.599 kb on - strand at 2833.602 kb on + strand at 2833.602 kb on + strand at 2833.619 kb on - strand at 2833.632 kb on + strand at 2833.867 kb on - strand, within hdfR at 2833.867 kb on - strand, within hdfR at 2833.919 kb on + strand, within hdfR at 2833.919 kb on + strand, within hdfR at 2833.920 kb on - strand, within hdfR at 2833.932 kb on - strand, within hdfR at 2833.973 kb on + strand, within hdfR at 2833.973 kb on + strand, within hdfR at 2833.973 kb on + strand, within hdfR at 2833.974 kb on - strand, within hdfR at 2833.974 kb on - strand, within hdfR at 2833.974 kb on - strand, within hdfR at 2833.974 kb on - strand, within hdfR at 2833.974 kb on - strand, within hdfR at 2833.979 kb on + strand, within hdfR at 2833.979 kb on + strand, within hdfR at 2834.022 kb on - strand, within hdfR at 2834.030 kb on - strand, within hdfR at 2834.102 kb on + strand, within hdfR at 2834.102 kb on + strand, within hdfR at 2834.135 kb on + strand, within hdfR at 2834.138 kb on + strand, within hdfR at 2834.138 kb on + strand, within hdfR at 2834.174 kb on + strand, within hdfR at 2834.175 kb on - strand, within hdfR at 2834.207 kb on + strand, within hdfR at 2834.208 kb on - strand, within hdfR at 2834.211 kb on + strand, within hdfR at 2834.211 kb on + strand, within hdfR at 2834.211 kb on + strand, within hdfR at 2834.212 kb on - strand, within hdfR at 2834.431 kb on + strand at 2834.450 kb on + strand at 2834.457 kb on - strand at 2834.473 kb on + strand at 2834.503 kb on - strand at 2834.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,832,759 + yifB OKFHMN_14665 0.72 -0.8 2,832,759 + yifB OKFHMN_14665 0.72 -0.3 2,832,759 + yifB OKFHMN_14665 0.72 +0.4 2,832,759 + yifB OKFHMN_14665 0.72 +0.7 2,832,759 + yifB OKFHMN_14665 0.72 -1.2 2,832,759 + yifB OKFHMN_14665 0.72 -0.7 2,832,759 + yifB OKFHMN_14665 0.72 +0.7 2,832,759 + yifB OKFHMN_14665 0.72 -3.3 2,832,759 + yifB OKFHMN_14665 0.72 -0.4 2,832,759 + yifB OKFHMN_14665 0.72 -0.9 2,832,760 - yifB OKFHMN_14665 0.72 +1.0 2,832,760 - yifB OKFHMN_14665 0.72 +0.0 2,832,760 - yifB OKFHMN_14665 0.72 -2.9 2,832,760 - yifB OKFHMN_14665 0.72 +0.2 2,832,760 - yifB OKFHMN_14665 0.72 +0.5 2,832,760 - yifB OKFHMN_14665 0.72 +1.0 2,832,760 - yifB OKFHMN_14665 0.72 -0.8 2,832,760 - yifB OKFHMN_14665 0.72 +0.2 2,832,760 - yifB OKFHMN_14665 0.72 -2.6 2,832,848 - yifB OKFHMN_14665 0.78 -0.2 2,832,884 - yifB OKFHMN_14665 0.80 -0.2 2,832,894 + yifB OKFHMN_14665 0.81 -2.7 2,832,894 + yifB OKFHMN_14665 0.81 +1.2 2,832,894 + yifB OKFHMN_14665 0.81 -0.2 2,832,895 - yifB OKFHMN_14665 0.81 +0.3 2,832,952 + yifB OKFHMN_14665 0.85 -0.2 2,833,084 + +0.3 2,833,084 + +1.7 2,833,132 + +0.4 2,833,132 + -0.6 2,833,132 + +1.1 2,833,133 - -0.1 2,833,136 + -1.1 2,833,176 + +0.1 2,833,176 + -2.7 2,833,177 - -2.6 2,833,212 + -0.8 2,833,212 + -0.5 2,833,231 - -2.4 2,833,307 - maoP OKFHMN_14670 0.28 -3.1 2,833,308 + maoP OKFHMN_14670 0.29 +0.1 2,833,308 + maoP OKFHMN_14670 0.29 -0.2 2,833,309 - maoP OKFHMN_14670 0.29 -0.9 2,833,309 - maoP OKFHMN_14670 0.29 -1.2 2,833,309 - maoP OKFHMN_14670 0.29 -1.6 2,833,361 + maoP OKFHMN_14670 0.44 -1.3 2,833,362 - maoP OKFHMN_14670 0.45 +1.1 2,833,368 - maoP OKFHMN_14670 0.46 -1.3 2,833,431 + maoP OKFHMN_14670 0.65 -3.4 2,833,431 + maoP OKFHMN_14670 0.65 -4.3 2,833,431 + maoP OKFHMN_14670 0.65 -0.8 2,833,432 - maoP OKFHMN_14670 0.65 -0.9 2,833,507 + maoP OKFHMN_14670 0.87 -0.7 2,833,507 + maoP OKFHMN_14670 0.87 -1.5 2,833,508 - maoP OKFHMN_14670 0.88 -0.5 2,833,508 - maoP OKFHMN_14670 0.88 -1.5 2,833,562 + -0.8 2,833,563 - -4.9 2,833,583 + -3.8 2,833,599 - -1.3 2,833,599 - -0.9 2,833,602 + -2.6 2,833,602 + +0.2 2,833,619 - -2.1 2,833,632 + -1.2 2,833,867 - hdfR OKFHMN_14675 0.24 -0.7 2,833,867 - hdfR OKFHMN_14675 0.24 -1.5 2,833,919 + hdfR OKFHMN_14675 0.30 -0.7 2,833,919 + hdfR OKFHMN_14675 0.30 -1.1 2,833,920 - hdfR OKFHMN_14675 0.30 -1.3 2,833,932 - hdfR OKFHMN_14675 0.31 -0.4 2,833,973 + hdfR OKFHMN_14675 0.36 -0.0 2,833,973 + hdfR OKFHMN_14675 0.36 -0.9 2,833,973 + hdfR OKFHMN_14675 0.36 -2.5 2,833,974 - hdfR OKFHMN_14675 0.36 -2.3 2,833,974 - hdfR OKFHMN_14675 0.36 +0.4 2,833,974 - hdfR OKFHMN_14675 0.36 +0.4 2,833,974 - hdfR OKFHMN_14675 0.36 -3.1 2,833,974 - hdfR OKFHMN_14675 0.36 -1.7 2,833,979 + hdfR OKFHMN_14675 0.37 -0.4 2,833,979 + hdfR OKFHMN_14675 0.37 -0.5 2,834,022 - hdfR OKFHMN_14675 0.42 +0.0 2,834,030 - hdfR OKFHMN_14675 0.43 +1.9 2,834,102 + hdfR OKFHMN_14675 0.52 -1.5 2,834,102 + hdfR OKFHMN_14675 0.52 +0.4 2,834,135 + hdfR OKFHMN_14675 0.56 +1.0 2,834,138 + hdfR OKFHMN_14675 0.56 -2.8 2,834,138 + hdfR OKFHMN_14675 0.56 -3.2 2,834,174 + hdfR OKFHMN_14675 0.60 -1.2 2,834,175 - hdfR OKFHMN_14675 0.60 -3.5 2,834,207 + hdfR OKFHMN_14675 0.64 -1.2 2,834,208 - hdfR OKFHMN_14675 0.64 -1.9 2,834,211 + hdfR OKFHMN_14675 0.65 -0.3 2,834,211 + hdfR OKFHMN_14675 0.65 +0.5 2,834,211 + hdfR OKFHMN_14675 0.65 -1.2 2,834,212 - hdfR OKFHMN_14675 0.65 -3.2 2,834,431 + -1.4 2,834,450 + -4.4 2,834,457 - -0.6 2,834,473 + -0.7 2,834,503 - -1.7 2,834,506 - +1.1
Or see this region's nucleotide sequence