Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsB and ydaE are separated by 54 nucleotides ydaE and fba are separated by 14 nucleotides fba and rbsK overlap by 4 nucleotides rbsK and ompR overlap by 4 nucleotides
OKFHMN_12720: rbsB - transcriptional regulator, at 2,437,807 to 2,438,748
rbsB
OKFHMN_12725: ydaE - D-lyxose/D-mannose isomerase, at 2,438,803 to 2,439,486
ydaE
OKFHMN_12730: fba - ketose 1,6-bisphosphate aldolase, at 2,439,501 to 2,440,361
fba
OKFHMN_12735: rbsK - sugar kinase, at 2,440,358 to 2,441,359
rbsK
OKFHMN_12740: ompR - DNA-binding response regulator, at 2,441,356 to 2,442,063
ompR
Position (kb)
2439
2440
2441 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2438.509 kb on - strand, within rbsB at 2438.509 kb on - strand, within rbsB at 2438.746 kb on + strand at 2438.817 kb on + strand at 2438.819 kb on + strand at 2438.838 kb on + strand at 2438.867 kb on + strand at 2438.867 kb on + strand at 2438.867 kb on + strand at 2438.868 kb on - strand at 2438.868 kb on - strand at 2439.020 kb on + strand, within ydaE at 2439.049 kb on + strand, within ydaE at 2439.050 kb on - strand, within ydaE at 2439.138 kb on + strand, within ydaE at 2439.138 kb on + strand, within ydaE at 2439.139 kb on - strand, within ydaE at 2439.161 kb on + strand, within ydaE at 2439.162 kb on - strand, within ydaE at 2439.216 kb on + strand, within ydaE at 2439.227 kb on + strand, within ydaE at 2439.228 kb on - strand, within ydaE at 2439.289 kb on - strand, within ydaE at 2439.289 kb on - strand, within ydaE at 2439.455 kb on + strand at 2439.455 kb on + strand at 2439.456 kb on - strand at 2439.456 kb on - strand at 2439.462 kb on + strand at 2439.500 kb on + strand at 2439.560 kb on - strand at 2439.669 kb on - strand, within fba at 2439.669 kb on - strand, within fba at 2439.939 kb on + strand, within fba at 2439.940 kb on - strand, within fba at 2440.123 kb on + strand, within fba at 2440.124 kb on - strand, within fba at 2440.124 kb on - strand, within fba at 2440.283 kb on - strand at 2440.283 kb on - strand at 2440.334 kb on + strand at 2440.369 kb on + strand at 2440.393 kb on + strand at 2440.394 kb on - strand at 2440.394 kb on - strand at 2440.394 kb on - strand at 2440.492 kb on + strand, within rbsK at 2440.577 kb on + strand, within rbsK at 2440.578 kb on - strand, within rbsK at 2440.602 kb on - strand, within rbsK at 2440.634 kb on - strand, within rbsK at 2440.782 kb on + strand, within rbsK at 2440.782 kb on + strand, within rbsK at 2440.783 kb on - strand, within rbsK at 2440.783 kb on - strand, within rbsK at 2440.783 kb on - strand, within rbsK at 2440.861 kb on + strand, within rbsK at 2440.862 kb on - strand, within rbsK at 2441.036 kb on - strand, within rbsK at 2441.249 kb on - strand, within rbsK at 2441.296 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,438,509 - rbsB OKFHMN_12720 0.75 +1.8 2,438,509 - rbsB OKFHMN_12720 0.75 -0.2 2,438,746 + -1.4 2,438,817 + -0.1 2,438,819 + -1.7 2,438,838 + +0.6 2,438,867 + +1.0 2,438,867 + -1.0 2,438,867 + +0.5 2,438,868 - -1.2 2,438,868 - -0.0 2,439,020 + ydaE OKFHMN_12725 0.32 -2.1 2,439,049 + ydaE OKFHMN_12725 0.36 -0.1 2,439,050 - ydaE OKFHMN_12725 0.36 -0.2 2,439,138 + ydaE OKFHMN_12725 0.49 -0.1 2,439,138 + ydaE OKFHMN_12725 0.49 +0.4 2,439,139 - ydaE OKFHMN_12725 0.49 -0.8 2,439,161 + ydaE OKFHMN_12725 0.52 +1.2 2,439,162 - ydaE OKFHMN_12725 0.52 +0.9 2,439,216 + ydaE OKFHMN_12725 0.60 +0.3 2,439,227 + ydaE OKFHMN_12725 0.62 -0.7 2,439,228 - ydaE OKFHMN_12725 0.62 -0.4 2,439,289 - ydaE OKFHMN_12725 0.71 -0.5 2,439,289 - ydaE OKFHMN_12725 0.71 -0.4 2,439,455 + +0.7 2,439,455 + -0.5 2,439,456 - +0.5 2,439,456 - -1.4 2,439,462 + -1.3 2,439,500 + -0.8 2,439,560 - -0.0 2,439,669 - fba OKFHMN_12730 0.20 -3.8 2,439,669 - fba OKFHMN_12730 0.20 +0.9 2,439,939 + fba OKFHMN_12730 0.51 -0.3 2,439,940 - fba OKFHMN_12730 0.51 +0.2 2,440,123 + fba OKFHMN_12730 0.72 +0.9 2,440,124 - fba OKFHMN_12730 0.72 +0.5 2,440,124 - fba OKFHMN_12730 0.72 -0.5 2,440,283 - +2.3 2,440,283 - -0.4 2,440,334 + -1.7 2,440,369 + +0.6 2,440,393 + -0.5 2,440,394 - -0.3 2,440,394 - +0.0 2,440,394 - -0.3 2,440,492 + rbsK OKFHMN_12735 0.13 +1.1 2,440,577 + rbsK OKFHMN_12735 0.22 -1.8 2,440,578 - rbsK OKFHMN_12735 0.22 +0.1 2,440,602 - rbsK OKFHMN_12735 0.24 +0.3 2,440,634 - rbsK OKFHMN_12735 0.28 -0.3 2,440,782 + rbsK OKFHMN_12735 0.42 -0.0 2,440,782 + rbsK OKFHMN_12735 0.42 +1.7 2,440,783 - rbsK OKFHMN_12735 0.42 -0.5 2,440,783 - rbsK OKFHMN_12735 0.42 +1.4 2,440,783 - rbsK OKFHMN_12735 0.42 +1.1 2,440,861 + rbsK OKFHMN_12735 0.50 +0.7 2,440,862 - rbsK OKFHMN_12735 0.50 -0.1 2,441,036 - rbsK OKFHMN_12735 0.68 +0.2 2,441,249 - rbsK OKFHMN_12735 0.89 -0.5 2,441,296 - +0.3
Or see this region's nucleotide sequence