Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt queG and yjeV overlap by 18 nucleotides yjeV and Gly_trna are separated by 233 nucleotides Gly_trna and Gly_trna are separated by 35 nucleotides Gly_trna and Gly_trna are separated by 35 nucleotides Gly_trna and Gly_trna are separated by 36 nucleotides Gly_trna and orn are separated by 210 nucleotides orn and rsgA are separated by 94 nucleotides
OKFHMN_12285: queG - tRNA epoxyqueuosine(34) reductase QueG, at 2,353,734 to 2,354,873
queG
OKFHMN_12290: yjeV - Uncharacterized protein YjeV, at 2,354,856 to 2,354,909
yjeV
OKFHMN_12295: Gly_trna - tRNA-Gly(gcc), at 2,355,143 to 2,355,218
_trna
OKFHMN_12300: Gly_trna - tRNA-Gly(gcc), at 2,355,254 to 2,355,329
_trna
OKFHMN_12305: Gly_trna - tRNA-Gly(gcc), at 2,355,365 to 2,355,440
_trna
OKFHMN_12310: Gly_trna - tRNA-Gly(gcc), at 2,355,477 to 2,355,552
_trna
OKFHMN_12315: orn - oligoribonuclease, at 2,355,763 to 2,356,308
orn
OKFHMN_12320: rsgA - small ribosomal subunit biogenesis GTPase RsgA, at 2,356,403 to 2,357,455
rsgA
Position (kb)
2355
2356 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2354.552 kb on - strand, within queG at 2354.575 kb on + strand, within queG at 2354.634 kb on - strand, within queG at 2354.660 kb on + strand, within queG at 2354.674 kb on + strand, within queG at 2354.674 kb on + strand, within queG at 2354.755 kb on + strand, within queG at 2354.976 kb on + strand at 2354.977 kb on - strand at 2355.101 kb on - strand at 2355.555 kb on - strand at 2355.555 kb on - strand at 2355.555 kb on - strand at 2356.338 kb on - strand at 2356.338 kb on - strand at 2356.338 kb on - strand at 2356.338 kb on - strand at 2356.338 kb on - strand at 2356.358 kb on - strand at 2356.358 kb on - strand at 2356.358 kb on - strand at 2356.362 kb on + strand at 2356.362 kb on + strand at 2356.362 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,354,552 - queG OKFHMN_12285 0.72 +0.7 2,354,575 + queG OKFHMN_12285 0.74 -0.7 2,354,634 - queG OKFHMN_12285 0.79 -0.2 2,354,660 + queG OKFHMN_12285 0.81 -3.3 2,354,674 + queG OKFHMN_12285 0.82 -0.6 2,354,674 + queG OKFHMN_12285 0.82 +0.3 2,354,755 + queG OKFHMN_12285 0.90 +0.2 2,354,976 + +1.2 2,354,977 - +1.0 2,355,101 - -1.4 2,355,555 - -1.7 2,355,555 - +0.2 2,355,555 - +0.5 2,356,338 - -0.4 2,356,338 - -2.1 2,356,338 - -0.2 2,356,338 - -0.1 2,356,338 - +2.3 2,356,358 - +0.7 2,356,358 - +1.3 2,356,358 - -3.5 2,356,362 + -2.8 2,356,362 + -0.2 2,356,362 + +0.8
Or see this region's nucleotide sequence