Strain Fitness in Escherichia coli ECRC100 around OKFHMN_12075

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcycA and fklB are separated by 308 nucleotidesfklB and ytfB are separated by 217 nucleotidesytfB and insQ are separated by 134 nucleotides OKFHMN_12070: cycA - D-serine/D-alanine/glycine transporter, at 2,314,776 to 2,316,188 cycA OKFHMN_12075: fklB - FKBP-type peptidyl-prolyl cis-trans isomerase, at 2,316,497 to 2,317,117 fklB OKFHMN_12080: ytfB - cell division protein YtfB, at 2,317,335 to 2,317,973 ytfB OKFHMN_12085: insQ - Putative RNA-guided DNA endonuclease InsQ, at 2,318,108 to 2,319,256 insQ Position (kb) 2316 2317 2318Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2315.557 kb on - strand, within cycAat 2315.741 kb on + strand, within cycAat 2315.742 kb on - strand, within cycAat 2315.760 kb on + strand, within cycAat 2315.760 kb on + strand, within cycAat 2315.761 kb on - strand, within cycAat 2315.869 kb on + strand, within cycAat 2315.938 kb on + strand, within cycAat 2315.993 kb on + strand, within cycAat 2315.993 kb on + strand, within cycAat 2315.994 kb on - strand, within cycAat 2315.994 kb on - strand, within cycAat 2316.009 kb on - strand, within cycAat 2316.009 kb on - strand, within cycAat 2316.011 kb on - strand, within cycAat 2316.063 kb on - strandat 2316.107 kb on + strandat 2316.107 kb on + strandat 2316.108 kb on - strandat 2316.108 kb on - strandat 2316.175 kb on - strandat 2316.183 kb on + strandat 2316.204 kb on + strandat 2316.246 kb on + strandat 2316.246 kb on + strandat 2316.247 kb on - strandat 2316.247 kb on - strandat 2316.293 kb on + strandat 2316.293 kb on + strandat 2316.294 kb on - strandat 2316.307 kb on - strandat 2316.317 kb on - strandat 2316.328 kb on + strandat 2316.498 kb on + strandat 2316.596 kb on - strand, within fklBat 2316.610 kb on + strand, within fklBat 2316.611 kb on - strand, within fklBat 2316.617 kb on + strand, within fklBat 2316.738 kb on + strand, within fklBat 2316.739 kb on - strand, within fklBat 2316.739 kb on - strand, within fklBat 2316.760 kb on + strand, within fklBat 2316.761 kb on - strand, within fklBat 2316.761 kb on - strand, within fklBat 2316.823 kb on + strand, within fklBat 2316.823 kb on + strand, within fklBat 2316.858 kb on + strand, within fklBat 2316.859 kb on - strand, within fklBat 2317.139 kb on + strandat 2317.139 kb on + strandat 2317.139 kb on + strandat 2317.156 kb on + strandat 2317.226 kb on + strandat 2317.226 kb on + strandat 2317.227 kb on - strandat 2317.227 kb on - strandat 2317.240 kb on - strandat 2317.245 kb on + strandat 2317.246 kb on - strandat 2317.276 kb on + strandat 2317.282 kb on + strandat 2317.287 kb on + strandat 2317.288 kb on - strandat 2317.293 kb on + strandat 2317.293 kb on + strandat 2317.294 kb on - strandat 2317.299 kb on - strandat 2317.299 kb on - strandat 2317.443 kb on - strand, within ytfBat 2317.456 kb on + strand, within ytfBat 2317.456 kb on + strand, within ytfBat 2317.490 kb on + strand, within ytfBat 2317.528 kb on + strand, within ytfBat 2317.529 kb on - strand, within ytfBat 2317.532 kb on + strand, within ytfBat 2317.582 kb on + strand, within ytfBat 2317.583 kb on - strand, within ytfBat 2317.583 kb on - strand, within ytfBat 2317.634 kb on + strand, within ytfBat 2317.634 kb on + strand, within ytfBat 2317.635 kb on - strand, within ytfBat 2317.636 kb on + strand, within ytfBat 2317.636 kb on + strand, within ytfBat 2317.725 kb on + strand, within ytfBat 2317.725 kb on + strand, within ytfBat 2317.795 kb on - strand, within ytfBat 2317.810 kb on + strand, within ytfBat 2317.810 kb on + strand, within ytfBat 2317.811 kb on - strand, within ytfBat 2317.959 kb on - strandat 2317.971 kb on + strandat 2317.999 kb on - strandat 2317.999 kb on - strandat 2317.999 kb on - strandat 2318.022 kb on + strandat 2318.023 kb on - strandat 2318.056 kb on - strandat 2318.056 kb on - strandat 2318.056 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,315,557 - cycA OKFHMN_12070 0.55 -0.6
2,315,741 + cycA OKFHMN_12070 0.68 -0.3
2,315,742 - cycA OKFHMN_12070 0.68 +0.1
2,315,760 + cycA OKFHMN_12070 0.70 +0.1
2,315,760 + cycA OKFHMN_12070 0.70 -0.5
2,315,761 - cycA OKFHMN_12070 0.70 +0.5
2,315,869 + cycA OKFHMN_12070 0.77 -0.3
2,315,938 + cycA OKFHMN_12070 0.82 -1.7
2,315,993 + cycA OKFHMN_12070 0.86 -1.1
2,315,993 + cycA OKFHMN_12070 0.86 -0.3
2,315,994 - cycA OKFHMN_12070 0.86 +0.5
2,315,994 - cycA OKFHMN_12070 0.86 +0.1
2,316,009 - cycA OKFHMN_12070 0.87 +0.4
2,316,009 - cycA OKFHMN_12070 0.87 -1.3
2,316,011 - cycA OKFHMN_12070 0.87 -0.1
2,316,063 - -1.4
2,316,107 + +0.5
2,316,107 + -1.4
2,316,108 - -1.1
2,316,108 - +0.3
2,316,175 - -0.2
2,316,183 + +0.9
2,316,204 + -0.2
2,316,246 + +0.1
2,316,246 + -1.1
2,316,247 - -1.1
2,316,247 - +0.3
2,316,293 + +0.5
2,316,293 + +0.2
2,316,294 - -0.5
2,316,307 - -0.8
2,316,317 - +0.0
2,316,328 + -0.2
2,316,498 + -0.0
2,316,596 - fklB OKFHMN_12075 0.16 +0.0
2,316,610 + fklB OKFHMN_12075 0.18 +0.2
2,316,611 - fklB OKFHMN_12075 0.18 +0.3
2,316,617 + fklB OKFHMN_12075 0.19 +0.4
2,316,738 + fklB OKFHMN_12075 0.39 -3.5
2,316,739 - fklB OKFHMN_12075 0.39 +0.4
2,316,739 - fklB OKFHMN_12075 0.39 -0.1
2,316,760 + fklB OKFHMN_12075 0.42 +4.2
2,316,761 - fklB OKFHMN_12075 0.43 -1.2
2,316,761 - fklB OKFHMN_12075 0.43 +0.2
2,316,823 + fklB OKFHMN_12075 0.52 -0.0
2,316,823 + fklB OKFHMN_12075 0.52 -0.3
2,316,858 + fklB OKFHMN_12075 0.58 +0.2
2,316,859 - fklB OKFHMN_12075 0.58 -0.3
2,317,139 + -0.3
2,317,139 + +0.8
2,317,139 + +0.4
2,317,156 + -0.1
2,317,226 + +1.6
2,317,226 + -0.6
2,317,227 - -0.5
2,317,227 - +0.2
2,317,240 - -0.5
2,317,245 + -0.1
2,317,246 - +0.5
2,317,276 + -1.3
2,317,282 + -0.1
2,317,287 + -0.5
2,317,288 - +0.5
2,317,293 + -0.2
2,317,293 + +0.3
2,317,294 - +0.3
2,317,299 - +1.0
2,317,299 - -0.0
2,317,443 - ytfB OKFHMN_12080 0.17 -0.2
2,317,456 + ytfB OKFHMN_12080 0.19 -0.3
2,317,456 + ytfB OKFHMN_12080 0.19 -0.3
2,317,490 + ytfB OKFHMN_12080 0.24 +0.8
2,317,528 + ytfB OKFHMN_12080 0.30 +0.4
2,317,529 - ytfB OKFHMN_12080 0.30 +0.5
2,317,532 + ytfB OKFHMN_12080 0.31 +0.5
2,317,582 + ytfB OKFHMN_12080 0.39 +0.6
2,317,583 - ytfB OKFHMN_12080 0.39 -2.7
2,317,583 - ytfB OKFHMN_12080 0.39 +0.5
2,317,634 + ytfB OKFHMN_12080 0.47 +0.5
2,317,634 + ytfB OKFHMN_12080 0.47 +0.4
2,317,635 - ytfB OKFHMN_12080 0.47 -1.3
2,317,636 + ytfB OKFHMN_12080 0.47 +1.0
2,317,636 + ytfB OKFHMN_12080 0.47 +0.1
2,317,725 + ytfB OKFHMN_12080 0.61 +0.6
2,317,725 + ytfB OKFHMN_12080 0.61 -0.4
2,317,795 - ytfB OKFHMN_12080 0.72 +0.4
2,317,810 + ytfB OKFHMN_12080 0.74 +0.3
2,317,810 + ytfB OKFHMN_12080 0.74 -0.4
2,317,811 - ytfB OKFHMN_12080 0.74 +2.6
2,317,959 - -2.5
2,317,971 + +0.9
2,317,999 - +0.0
2,317,999 - -1.4
2,317,999 - +0.6
2,318,022 + -0.3
2,318,023 - -1.4
2,318,056 - -0.2
2,318,056 - -0.4
2,318,056 - -1.3

Or see this region's nucleotide sequence