Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaC and yjjA are separated by 48 nucleotides yjjA and opgB are separated by 253 nucleotides opgB and lgoD are separated by 136 nucleotides
OKFHMN_11345: dnaC - DNA replication protein DnaC, at 2,142,290 to 2,143,027
dnaC
OKFHMN_11350: yjjA - Uncharacterized protein YjjA, at 2,143,076 to 2,143,570
yjjA
OKFHMN_11355: opgB - phosphatidylglycerol--membrane-oligosaccharide glycerophosphotransferase, at 2,143,824 to 2,146,115
opgB
OKFHMN_11360: lgoD - L-galactonate oxidoreductase, at 2,146,252 to 2,147,274
lgoD
Position (kb)
2143
2144
2145
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2143.025 kb on + strand at 2143.046 kb on + strand at 2143.046 kb on + strand at 2143.047 kb on - strand at 2143.072 kb on - strand at 2143.247 kb on - strand, within yjjA at 2143.247 kb on - strand, within yjjA at 2143.256 kb on - strand, within yjjA at 2143.761 kb on - strand at 2143.784 kb on - strand at 2143.808 kb on + strand at 2143.872 kb on + strand at 2143.872 kb on + strand at 2143.872 kb on + strand at 2143.872 kb on + strand at 2143.893 kb on - strand at 2143.893 kb on - strand at 2143.893 kb on - strand at 2143.918 kb on + strand at 2143.934 kb on - strand at 2143.950 kb on - strand at 2143.971 kb on + strand at 2143.991 kb on + strand at 2144.008 kb on - strand at 2144.015 kb on - strand at 2144.215 kb on + strand, within opgB at 2144.268 kb on + strand, within opgB at 2144.269 kb on - strand, within opgB at 2144.272 kb on - strand, within opgB at 2144.274 kb on - strand, within opgB at 2144.319 kb on + strand, within opgB at 2144.319 kb on + strand, within opgB at 2144.319 kb on + strand, within opgB at 2144.320 kb on - strand, within opgB at 2144.321 kb on + strand, within opgB at 2144.326 kb on - strand, within opgB at 2144.350 kb on - strand, within opgB at 2144.668 kb on + strand, within opgB at 2144.669 kb on - strand, within opgB at 2144.669 kb on - strand, within opgB at 2144.669 kb on - strand, within opgB at 2144.712 kb on + strand, within opgB at 2144.874 kb on + strand, within opgB at 2144.874 kb on + strand, within opgB at 2144.915 kb on - strand, within opgB at 2144.946 kb on - strand, within opgB at 2144.981 kb on + strand, within opgB at 2144.982 kb on - strand, within opgB at 2145.167 kb on + strand, within opgB at 2145.168 kb on - strand, within opgB at 2145.168 kb on - strand, within opgB at 2145.171 kb on + strand, within opgB at 2145.172 kb on - strand, within opgB at 2145.230 kb on + strand, within opgB at 2145.415 kb on - strand, within opgB at 2145.624 kb on - strand, within opgB at 2145.624 kb on - strand, within opgB at 2145.693 kb on + strand, within opgB at 2145.693 kb on + strand, within opgB at 2145.717 kb on - strand, within opgB at 2145.753 kb on - strand, within opgB at 2146.043 kb on + strand at 2146.131 kb on + strand at 2146.131 kb on + strand at 2146.132 kb on - strand at 2146.140 kb on + strand at 2146.171 kb on - strand at 2146.238 kb on - strand at 2146.241 kb on - strand at 2146.265 kb on - strand at 2146.273 kb on - strand at 2146.307 kb on + strand at 2146.502 kb on - strand, within lgoD at 2146.558 kb on - strand, within lgoD at 2146.828 kb on - strand, within lgoD at 2146.866 kb on + strand, within lgoD at 2146.908 kb on - strand, within lgoD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,143,025 + -1.4 2,143,046 + -0.0 2,143,046 + -0.5 2,143,047 - +0.2 2,143,072 - +0.2 2,143,247 - yjjA OKFHMN_11350 0.35 -0.9 2,143,247 - yjjA OKFHMN_11350 0.35 +2.5 2,143,256 - yjjA OKFHMN_11350 0.36 +0.3 2,143,761 - +0.8 2,143,784 - -0.1 2,143,808 + -0.6 2,143,872 + +2.7 2,143,872 + -1.0 2,143,872 + +2.8 2,143,872 + +1.0 2,143,893 - +0.4 2,143,893 - -0.2 2,143,893 - -0.6 2,143,918 + +0.4 2,143,934 - +0.5 2,143,950 - -0.9 2,143,971 + +0.6 2,143,991 + -0.5 2,144,008 - -1.4 2,144,015 - -1.5 2,144,215 + opgB OKFHMN_11355 0.17 +0.1 2,144,268 + opgB OKFHMN_11355 0.19 -1.7 2,144,269 - opgB OKFHMN_11355 0.19 -0.0 2,144,272 - opgB OKFHMN_11355 0.20 +0.7 2,144,274 - opgB OKFHMN_11355 0.20 +0.2 2,144,319 + opgB OKFHMN_11355 0.22 +0.1 2,144,319 + opgB OKFHMN_11355 0.22 +0.2 2,144,319 + opgB OKFHMN_11355 0.22 -3.1 2,144,320 - opgB OKFHMN_11355 0.22 +0.4 2,144,321 + opgB OKFHMN_11355 0.22 -0.1 2,144,326 - opgB OKFHMN_11355 0.22 -0.6 2,144,350 - opgB OKFHMN_11355 0.23 +0.2 2,144,668 + opgB OKFHMN_11355 0.37 +0.6 2,144,669 - opgB OKFHMN_11355 0.37 +0.2 2,144,669 - opgB OKFHMN_11355 0.37 +1.4 2,144,669 - opgB OKFHMN_11355 0.37 -1.0 2,144,712 + opgB OKFHMN_11355 0.39 -0.7 2,144,874 + opgB OKFHMN_11355 0.46 +0.4 2,144,874 + opgB OKFHMN_11355 0.46 +0.4 2,144,915 - opgB OKFHMN_11355 0.48 -3.3 2,144,946 - opgB OKFHMN_11355 0.49 +0.6 2,144,981 + opgB OKFHMN_11355 0.50 +0.6 2,144,982 - opgB OKFHMN_11355 0.51 -1.7 2,145,167 + opgB OKFHMN_11355 0.59 -0.2 2,145,168 - opgB OKFHMN_11355 0.59 +0.1 2,145,168 - opgB OKFHMN_11355 0.59 +0.5 2,145,171 + opgB OKFHMN_11355 0.59 -0.8 2,145,172 - opgB OKFHMN_11355 0.59 -0.9 2,145,230 + opgB OKFHMN_11355 0.61 -0.1 2,145,415 - opgB OKFHMN_11355 0.69 +0.3 2,145,624 - opgB OKFHMN_11355 0.79 +0.3 2,145,624 - opgB OKFHMN_11355 0.79 +1.7 2,145,693 + opgB OKFHMN_11355 0.82 -1.2 2,145,693 + opgB OKFHMN_11355 0.82 -0.9 2,145,717 - opgB OKFHMN_11355 0.83 +0.2 2,145,753 - opgB OKFHMN_11355 0.84 +0.2 2,146,043 + -0.1 2,146,131 + -0.4 2,146,131 + -0.1 2,146,132 - +0.9 2,146,140 + +0.1 2,146,171 - +1.2 2,146,238 - -0.7 2,146,241 - -2.9 2,146,265 - +0.0 2,146,273 - +0.5 2,146,307 + -0.4 2,146,502 - lgoD OKFHMN_11360 0.24 +0.5 2,146,558 - lgoD OKFHMN_11360 0.30 +0.7 2,146,828 - lgoD OKFHMN_11360 0.56 +0.6 2,146,866 + lgoD OKFHMN_11360 0.60 +0.3 2,146,908 - lgoD OKFHMN_11360 0.64 +0.6
Or see this region's nucleotide sequence