Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaA and yaaX are separated by 152 nucleotides yaaX and thrC are separated by 213 nucleotides thrC and thrB are separated by 0 nucleotides thrB and thrA are separated by 1 nucleotides
OKFHMN_11090: yaaA - peroxide stress protein YaaA, at 2,096,696 to 2,097,472
yaaA
OKFHMN_11095: yaaX - Uncharacterized protein YaaX, at 2,097,625 to 2,097,921
yaaX
OKFHMN_11100: thrC - threonine synthase, at 2,098,135 to 2,099,421
thrC
OKFHMN_11105: thrB - homoserine kinase, at 2,099,422 to 2,100,354
thrB
OKFHMN_11110: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 2,100,356 to 2,102,818
thrA
Position (kb)
2098
2099
2100 Strain fitness (log2 ratio)
-1
0
1
2 at 2097.142 kb on + strand, within yaaA at 2097.143 kb on - strand, within yaaA at 2097.143 kb on - strand, within yaaA at 2097.188 kb on - strand, within yaaA at 2097.199 kb on + strand, within yaaA at 2097.314 kb on + strand, within yaaA at 2097.407 kb on + strand at 2097.408 kb on - strand at 2097.408 kb on - strand at 2097.458 kb on + strand at 2097.627 kb on - strand at 2097.682 kb on - strand, within yaaX at 2097.740 kb on + strand, within yaaX at 2097.741 kb on - strand, within yaaX at 2097.801 kb on + strand, within yaaX at 2097.801 kb on + strand, within yaaX at 2097.838 kb on + strand, within yaaX at 2097.843 kb on + strand, within yaaX at 2097.843 kb on + strand, within yaaX at 2097.898 kb on + strand at 2097.903 kb on + strand at 2097.904 kb on - strand at 2097.904 kb on - strand at 2097.904 kb on - strand at 2097.935 kb on + strand at 2097.936 kb on - strand at 2097.936 kb on - strand at 2097.969 kb on + strand at 2097.992 kb on + strand at 2098.008 kb on - strand at 2098.096 kb on + strand at 2098.129 kb on + strand at 2098.129 kb on + strand at 2098.130 kb on - strand at 2098.137 kb on - strand at 2098.137 kb on - strand at 2098.189 kb on - strand at 2098.204 kb on + strand at 2098.276 kb on - strand, within thrC at 2098.354 kb on + strand, within thrC at 2098.746 kb on - strand, within thrC at 2098.749 kb on - strand, within thrC at 2098.978 kb on + strand, within thrC at 2098.986 kb on - strand, within thrC at 2099.083 kb on - strand, within thrC at 2099.083 kb on - strand, within thrC at 2099.104 kb on - strand, within thrC at 2099.403 kb on + strand at 2099.412 kb on - strand at 2099.496 kb on - strand at 2099.668 kb on - strand, within thrB at 2099.772 kb on - strand, within thrB at 2099.814 kb on + strand, within thrB at 2099.904 kb on + strand, within thrB at 2099.905 kb on - strand, within thrB at 2099.936 kb on + strand, within thrB at 2099.937 kb on - strand, within thrB at 2099.937 kb on - strand, within thrB at 2100.087 kb on + strand, within thrB at 2100.193 kb on - strand, within thrB at 2100.250 kb on + strand, within thrB at 2100.250 kb on + strand, within thrB at 2100.251 kb on - strand, within thrB at 2100.251 kb on - strand, within thrB at 2100.251 kb on - strand, within thrB at 2100.251 kb on - strand, within thrB at 2100.251 kb on - strand, within thrB at 2100.348 kb on + strand at 2100.366 kb on - strand at 2100.382 kb on + strand at 2100.383 kb on - strand at 2100.386 kb on + strand at 2100.386 kb on + strand at 2100.387 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,097,142 + yaaA OKFHMN_11090 0.57 -0.8 2,097,143 - yaaA OKFHMN_11090 0.58 +0.7 2,097,143 - yaaA OKFHMN_11090 0.58 +0.2 2,097,188 - yaaA OKFHMN_11090 0.63 -1.1 2,097,199 + yaaA OKFHMN_11090 0.65 +0.2 2,097,314 + yaaA OKFHMN_11090 0.80 +0.2 2,097,407 + +0.2 2,097,408 - -1.3 2,097,408 - -0.3 2,097,458 + +1.2 2,097,627 - +1.2 2,097,682 - yaaX OKFHMN_11095 0.19 +0.6 2,097,740 + yaaX OKFHMN_11095 0.39 -0.4 2,097,741 - yaaX OKFHMN_11095 0.39 +0.1 2,097,801 + yaaX OKFHMN_11095 0.59 +0.6 2,097,801 + yaaX OKFHMN_11095 0.59 +0.1 2,097,838 + yaaX OKFHMN_11095 0.72 +0.9 2,097,843 + yaaX OKFHMN_11095 0.73 +0.4 2,097,843 + yaaX OKFHMN_11095 0.73 -0.4 2,097,898 + +0.2 2,097,903 + +0.7 2,097,904 - -0.6 2,097,904 - -0.2 2,097,904 - +0.1 2,097,935 + +0.9 2,097,936 - +0.2 2,097,936 - +0.1 2,097,969 + -0.8 2,097,992 + +0.2 2,098,008 - -0.4 2,098,096 + -0.4 2,098,129 + -0.0 2,098,129 + +0.4 2,098,130 - +1.2 2,098,137 - +0.4 2,098,137 - +1.0 2,098,189 - -1.7 2,098,204 + -1.4 2,098,276 - thrC OKFHMN_11100 0.11 -1.7 2,098,354 + thrC OKFHMN_11100 0.17 +0.2 2,098,746 - thrC OKFHMN_11100 0.47 -0.7 2,098,749 - thrC OKFHMN_11100 0.48 +2.2 2,098,978 + thrC OKFHMN_11100 0.66 +0.0 2,098,986 - thrC OKFHMN_11100 0.66 -0.3 2,099,083 - thrC OKFHMN_11100 0.74 -0.4 2,099,083 - thrC OKFHMN_11100 0.74 +0.4 2,099,104 - thrC OKFHMN_11100 0.75 -0.9 2,099,403 + -1.0 2,099,412 - -0.7 2,099,496 - -0.4 2,099,668 - thrB OKFHMN_11105 0.26 -0.2 2,099,772 - thrB OKFHMN_11105 0.38 +0.1 2,099,814 + thrB OKFHMN_11105 0.42 -0.0 2,099,904 + thrB OKFHMN_11105 0.52 +0.5 2,099,905 - thrB OKFHMN_11105 0.52 -0.3 2,099,936 + thrB OKFHMN_11105 0.55 +0.5 2,099,937 - thrB OKFHMN_11105 0.55 +0.2 2,099,937 - thrB OKFHMN_11105 0.55 +0.0 2,100,087 + thrB OKFHMN_11105 0.71 -0.5 2,100,193 - thrB OKFHMN_11105 0.83 -0.3 2,100,250 + thrB OKFHMN_11105 0.89 -1.0 2,100,250 + thrB OKFHMN_11105 0.89 -0.0 2,100,251 - thrB OKFHMN_11105 0.89 -0.6 2,100,251 - thrB OKFHMN_11105 0.89 -0.1 2,100,251 - thrB OKFHMN_11105 0.89 +0.5 2,100,251 - thrB OKFHMN_11105 0.89 +0.4 2,100,251 - thrB OKFHMN_11105 0.89 +0.4 2,100,348 + +2.2 2,100,366 - -0.0 2,100,382 + -0.2 2,100,383 - +1.0 2,100,386 + +0.2 2,100,386 + -0.8 2,100,387 - -0.2
Or see this region's nucleotide sequence