Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fimA and panB are separated by 134 nucleotides panB and panC are separated by 11 nucleotides panC and rpnC are separated by 298 nucleotides
OKFHMN_10395: fimA - fimbrial protein, at 1,947,756 to 1,948,865
fimA
OKFHMN_10400: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,949,000 to 1,949,794
panB
OKFHMN_10405: panC - pantoate--beta-alanine ligase, at 1,949,806 to 1,950,657
panC
OKFHMN_10410: rpnC - recombination-promoting nuclease RpnC, at 1,950,956 to 1,951,852
rpnC
Position (kb)
1949
1950
1951 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1948.853 kb on + strand at 1948.853 kb on + strand at 1948.854 kb on - strand at 1948.867 kb on + strand at 1948.929 kb on + strand at 1948.930 kb on - strand at 1948.930 kb on - strand at 1948.930 kb on - strand at 1948.960 kb on + strand at 1948.982 kb on + strand at 1948.982 kb on + strand at 1949.035 kb on + strand at 1949.036 kb on - strand at 1949.036 kb on - strand at 1949.167 kb on + strand, within panB at 1949.168 kb on - strand, within panB at 1949.267 kb on + strand, within panB at 1949.324 kb on - strand, within panB at 1949.324 kb on - strand, within panB at 1949.398 kb on - strand, within panB at 1949.435 kb on - strand, within panB at 1949.491 kb on + strand, within panB at 1949.502 kb on + strand, within panB at 1949.508 kb on + strand, within panB at 1949.509 kb on - strand, within panB at 1949.509 kb on - strand, within panB at 1949.509 kb on - strand, within panB at 1949.570 kb on - strand, within panB at 1949.674 kb on + strand, within panB at 1949.738 kb on + strand at 1949.738 kb on + strand at 1949.738 kb on + strand at 1949.739 kb on - strand at 1949.739 kb on - strand at 1949.740 kb on + strand at 1949.740 kb on + strand at 1949.740 kb on + strand at 1949.740 kb on + strand at 1949.810 kb on + strand at 1949.859 kb on + strand at 1949.889 kb on + strand at 1949.890 kb on - strand at 1949.890 kb on - strand at 1950.101 kb on - strand, within panC at 1950.127 kb on + strand, within panC at 1950.127 kb on + strand, within panC at 1950.128 kb on - strand, within panC at 1950.336 kb on + strand, within panC at 1950.429 kb on - strand, within panC at 1950.480 kb on + strand, within panC at 1950.658 kb on + strand at 1950.658 kb on + strand at 1950.658 kb on + strand at 1950.659 kb on - strand at 1950.662 kb on + strand at 1950.662 kb on + strand at 1950.663 kb on - strand at 1950.663 kb on - strand at 1950.664 kb on + strand at 1950.783 kb on + strand at 1950.803 kb on - strand at 1950.803 kb on - strand at 1950.803 kb on - strand at 1950.865 kb on + strand at 1950.865 kb on + strand at 1950.894 kb on + strand at 1950.929 kb on + strand at 1950.957 kb on + strand at 1950.958 kb on - strand at 1950.958 kb on - strand at 1950.971 kb on - strand at 1951.132 kb on + strand, within rpnC at 1951.133 kb on - strand, within rpnC at 1951.133 kb on - strand, within rpnC at 1951.170 kb on + strand, within rpnC at 1951.171 kb on - strand, within rpnC at 1951.192 kb on + strand, within rpnC at 1951.192 kb on + strand, within rpnC at 1951.211 kb on - strand, within rpnC at 1951.212 kb on + strand, within rpnC at 1951.292 kb on + strand, within rpnC at 1951.293 kb on - strand, within rpnC at 1951.293 kb on - strand, within rpnC at 1951.322 kb on + strand, within rpnC at 1951.406 kb on - strand, within rpnC at 1951.407 kb on + strand, within rpnC at 1951.407 kb on + strand, within rpnC at 1951.515 kb on - strand, within rpnC at 1951.557 kb on - strand, within rpnC at 1951.559 kb on - strand, within rpnC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,948,853 + +0.3 1,948,853 + +0.6 1,948,854 - +1.2 1,948,867 + +1.6 1,948,929 + +0.0 1,948,930 - -0.2 1,948,930 - +0.5 1,948,930 - -0.6 1,948,960 + -0.6 1,948,982 + -0.0 1,948,982 + -0.7 1,949,035 + -2.4 1,949,036 - -1.3 1,949,036 - -2.0 1,949,167 + panB OKFHMN_10400 0.21 -1.7 1,949,168 - panB OKFHMN_10400 0.21 -0.7 1,949,267 + panB OKFHMN_10400 0.34 -1.7 1,949,324 - panB OKFHMN_10400 0.41 -0.6 1,949,324 - panB OKFHMN_10400 0.41 -0.7 1,949,398 - panB OKFHMN_10400 0.50 -1.4 1,949,435 - panB OKFHMN_10400 0.55 -0.8 1,949,491 + panB OKFHMN_10400 0.62 -1.2 1,949,502 + panB OKFHMN_10400 0.63 -0.9 1,949,508 + panB OKFHMN_10400 0.64 -0.7 1,949,509 - panB OKFHMN_10400 0.64 -1.8 1,949,509 - panB OKFHMN_10400 0.64 -2.8 1,949,509 - panB OKFHMN_10400 0.64 -2.7 1,949,570 - panB OKFHMN_10400 0.72 -1.7 1,949,674 + panB OKFHMN_10400 0.85 -2.8 1,949,738 + +0.0 1,949,738 + -0.9 1,949,738 + -1.1 1,949,739 - -1.9 1,949,739 - -1.2 1,949,740 + +0.1 1,949,740 + -3.1 1,949,740 + -0.7 1,949,740 + -1.4 1,949,810 + -1.1 1,949,859 + -0.5 1,949,889 + -0.6 1,949,890 - -0.9 1,949,890 - -1.0 1,950,101 - panC OKFHMN_10405 0.35 +0.6 1,950,127 + panC OKFHMN_10405 0.38 -1.7 1,950,127 + panC OKFHMN_10405 0.38 -3.4 1,950,128 - panC OKFHMN_10405 0.38 -0.6 1,950,336 + panC OKFHMN_10405 0.62 -0.4 1,950,429 - panC OKFHMN_10405 0.73 +0.1 1,950,480 + panC OKFHMN_10405 0.79 -1.2 1,950,658 + -1.2 1,950,658 + +0.4 1,950,658 + -0.9 1,950,659 - +0.3 1,950,662 + +0.0 1,950,662 + -3.0 1,950,663 - +0.7 1,950,663 - +0.7 1,950,664 + +0.6 1,950,783 + +0.2 1,950,803 - +0.2 1,950,803 - -0.3 1,950,803 - -0.2 1,950,865 + -2.1 1,950,865 + +0.1 1,950,894 + +0.6 1,950,929 + +1.0 1,950,957 + +0.8 1,950,958 - +0.5 1,950,958 - -1.3 1,950,971 - +0.2 1,951,132 + rpnC OKFHMN_10410 0.20 +0.3 1,951,133 - rpnC OKFHMN_10410 0.20 -0.3 1,951,133 - rpnC OKFHMN_10410 0.20 +0.7 1,951,170 + rpnC OKFHMN_10410 0.24 +0.1 1,951,171 - rpnC OKFHMN_10410 0.24 +1.7 1,951,192 + rpnC OKFHMN_10410 0.26 -1.2 1,951,192 + rpnC OKFHMN_10410 0.26 -0.2 1,951,211 - rpnC OKFHMN_10410 0.28 -1.6 1,951,212 + rpnC OKFHMN_10410 0.29 -0.5 1,951,292 + rpnC OKFHMN_10410 0.37 -1.3 1,951,293 - rpnC OKFHMN_10410 0.38 -0.4 1,951,293 - rpnC OKFHMN_10410 0.38 -1.3 1,951,322 + rpnC OKFHMN_10410 0.41 +0.2 1,951,406 - rpnC OKFHMN_10410 0.50 -0.6 1,951,407 + rpnC OKFHMN_10410 0.50 +0.1 1,951,407 + rpnC OKFHMN_10410 0.50 +0.2 1,951,515 - rpnC OKFHMN_10410 0.62 +2.0 1,951,557 - rpnC OKFHMN_10410 0.67 +1.8 1,951,559 - rpnC OKFHMN_10410 0.67 +0.6
Or see this region's nucleotide sequence