Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10100

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnlpE and arfB are separated by 13 nucleotidesarfB and yaeQ overlap by 4 nucleotidesyaeQ and yaeP are separated by 165 nucleotidesyaeP and rof overlap by 14 nucleotidesrof and tilS are separated by 52 nucleotides OKFHMN_10090: nlpE - envelope stress response activation lipoprotein NlpE, at 1,883,881 to 1,884,591 nlpE OKFHMN_10095: arfB - aminoacyl-tRNA hydrolase, at 1,884,605 to 1,885,015 arfB OKFHMN_10100: yaeQ - Uncharacterized protein YaeQ, at 1,885,012 to 1,885,557 yaeQ OKFHMN_10105: yaeP - YaeP family protein, at 1,885,723 to 1,885,923 yaeP OKFHMN_10110: rof - Rho-binding antiterminator, at 1,885,910 to 1,886,170 rof OKFHMN_10115: tilS - tRNA lysidine(34) synthetase TilS, at 1,886,223 to 1,887,518 tilS Position (kb) 1885 1886Strain fitness (log2 ratio) -3 -2 -1 0 1at 1884.127 kb on - strand, within nlpEat 1884.129 kb on - strand, within nlpEat 1884.135 kb on - strand, within nlpEat 1884.167 kb on - strand, within nlpEat 1884.194 kb on + strand, within nlpEat 1884.267 kb on - strand, within nlpEat 1884.296 kb on - strand, within nlpEat 1884.324 kb on + strand, within nlpEat 1884.325 kb on - strand, within nlpEat 1884.325 kb on - strand, within nlpEat 1884.508 kb on - strand, within nlpEat 1884.526 kb on - strandat 1884.529 kb on - strandat 1884.809 kb on + strand, within arfBat 1884.862 kb on - strand, within arfBat 1884.864 kb on + strand, within arfBat 1884.865 kb on - strand, within arfBat 1884.923 kb on - strand, within arfBat 1885.054 kb on - strandat 1885.059 kb on + strandat 1885.114 kb on - strand, within yaeQat 1885.233 kb on + strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.236 kb on + strand, within yaeQat 1885.236 kb on + strand, within yaeQat 1885.237 kb on - strand, within yaeQat 1885.238 kb on + strand, within yaeQat 1885.238 kb on + strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.242 kb on + strand, within yaeQat 1885.440 kb on + strand, within yaeQat 1885.599 kb on - strandat 1885.640 kb on - strandat 1885.641 kb on + strandat 1885.664 kb on + strandat 1885.664 kb on + strandat 1885.742 kb on + strandat 1885.757 kb on - strand, within yaePat 1885.781 kb on + strand, within yaePat 1885.782 kb on - strand, within yaePat 1885.787 kb on - strand, within yaePat 1885.924 kb on + strandat 1885.924 kb on + strandat 1885.925 kb on - strandat 1885.928 kb on + strandat 1885.928 kb on + strandat 1885.928 kb on + strandat 1885.929 kb on - strandat 1885.986 kb on + strand, within rofat 1886.066 kb on + strand, within rofat 1886.103 kb on + strand, within rofat 1886.103 kb on + strand, within rofat 1886.104 kb on - strand, within rofat 1886.120 kb on - strand, within rofat 1886.195 kb on + strandat 1886.206 kb on - strandat 1886.224 kb on + strandat 1886.225 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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1,884,127 - nlpE OKFHMN_10090 0.35 +0.8
1,884,129 - nlpE OKFHMN_10090 0.35 -0.7
1,884,135 - nlpE OKFHMN_10090 0.36 -0.3
1,884,167 - nlpE OKFHMN_10090 0.40 +0.6
1,884,194 + nlpE OKFHMN_10090 0.44 -2.6
1,884,267 - nlpE OKFHMN_10090 0.54 -0.1
1,884,296 - nlpE OKFHMN_10090 0.58 +0.5
1,884,324 + nlpE OKFHMN_10090 0.62 -1.1
1,884,325 - nlpE OKFHMN_10090 0.62 +0.3
1,884,325 - nlpE OKFHMN_10090 0.62 +0.1
1,884,508 - nlpE OKFHMN_10090 0.88 +0.0
1,884,526 - +1.2
1,884,529 - +1.2
1,884,809 + arfB OKFHMN_10095 0.50 +0.7
1,884,862 - arfB OKFHMN_10095 0.63 +0.2
1,884,864 + arfB OKFHMN_10095 0.63 -3.1
1,884,865 - arfB OKFHMN_10095 0.63 -1.8
1,884,923 - arfB OKFHMN_10095 0.77 -1.1
1,885,054 - -0.1
1,885,059 + +1.6
1,885,114 - yaeQ OKFHMN_10100 0.19 -0.8
1,885,233 + yaeQ OKFHMN_10100 0.40 +0.5
1,885,234 - yaeQ OKFHMN_10100 0.41 +0.5
1,885,234 - yaeQ OKFHMN_10100 0.41 +0.2
1,885,234 - yaeQ OKFHMN_10100 0.41 -0.2
1,885,236 + yaeQ OKFHMN_10100 0.41 -3.5
1,885,236 + yaeQ OKFHMN_10100 0.41 -1.9
1,885,237 - yaeQ OKFHMN_10100 0.41 +0.2
1,885,238 + yaeQ OKFHMN_10100 0.41 +0.4
1,885,238 + yaeQ OKFHMN_10100 0.41 +0.1
1,885,239 - yaeQ OKFHMN_10100 0.42 +0.6
1,885,239 - yaeQ OKFHMN_10100 0.42 -0.2
1,885,239 - yaeQ OKFHMN_10100 0.42 +0.2
1,885,242 + yaeQ OKFHMN_10100 0.42 +1.6
1,885,440 + yaeQ OKFHMN_10100 0.78 -0.9
1,885,599 - -3.2
1,885,640 - +0.6
1,885,641 + +1.3
1,885,664 + +0.1
1,885,664 + -0.8
1,885,742 + +0.5
1,885,757 - yaeP OKFHMN_10105 0.17 -1.7
1,885,781 + yaeP OKFHMN_10105 0.29 -0.5
1,885,782 - yaeP OKFHMN_10105 0.29 +0.8
1,885,787 - yaeP OKFHMN_10105 0.32 +0.1
1,885,924 + +0.1
1,885,924 + +1.1
1,885,925 - +1.2
1,885,928 + -2.0
1,885,928 + +0.3
1,885,928 + +0.3
1,885,929 - -0.0
1,885,986 + rof OKFHMN_10110 0.29 +0.8
1,886,066 + rof OKFHMN_10110 0.60 -0.5
1,886,103 + rof OKFHMN_10110 0.74 +0.1
1,886,103 + rof OKFHMN_10110 0.74 -0.4
1,886,104 - rof OKFHMN_10110 0.74 -0.8
1,886,120 - rof OKFHMN_10110 0.80 +0.3
1,886,195 + +0.5
1,886,206 - +0.2
1,886,224 + +1.0
1,886,225 - +0.3

Or see this region's nucleotide sequence