Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and prfH are separated by 56 nucleotides prfH and ykfJ overlap by 32 nucleotides ykfJ and yafP are separated by 306 nucleotides
OKFHMN_09700: pepD - cytosol nonspecific dipeptidase, at 1,807,033 to 1,808,490
pepD
OKFHMN_09705: prfH - peptide chain release factor H, at 1,808,547 to 1,809,047
prfH
OKFHMN_09710: ykfJ - Putative uncharacterized protein YkfJ, at 1,809,016 to 1,809,282
ykfJ
OKFHMN_09715: yafP - GNAT family N-acetyltransferase, at 1,809,589 to 1,809,966
yafP
Position (kb)
1808
1809
1810 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1807.649 kb on + strand, within pepD at 1807.875 kb on + strand, within pepD at 1807.879 kb on + strand, within pepD at 1807.879 kb on + strand, within pepD at 1807.880 kb on - strand, within pepD at 1807.992 kb on + strand, within pepD at 1807.992 kb on + strand, within pepD at 1807.993 kb on - strand, within pepD at 1808.105 kb on + strand, within pepD at 1808.106 kb on - strand, within pepD at 1808.153 kb on - strand, within pepD at 1808.218 kb on + strand, within pepD at 1808.219 kb on - strand, within pepD at 1808.241 kb on + strand, within pepD at 1808.241 kb on + strand, within pepD at 1808.276 kb on - strand, within pepD at 1808.313 kb on + strand, within pepD at 1808.435 kb on + strand at 1808.436 kb on - strand at 1808.444 kb on - strand at 1808.548 kb on + strand at 1808.549 kb on - strand at 1809.127 kb on + strand, within ykfJ at 1809.182 kb on - strand, within ykfJ at 1809.182 kb on - strand, within ykfJ at 1809.232 kb on - strand, within ykfJ at 1809.260 kb on + strand at 1809.261 kb on - strand at 1809.272 kb on + strand at 1809.273 kb on - strand at 1809.273 kb on - strand at 1809.284 kb on + strand at 1809.296 kb on - strand at 1809.312 kb on - strand at 1809.352 kb on - strand at 1809.409 kb on + strand at 1809.569 kb on - strand at 1809.606 kb on - strand at 1809.804 kb on + strand, within yafP at 1809.811 kb on - strand, within yafP at 1809.813 kb on - strand, within yafP at 1810.024 kb on + strand at 1810.030 kb on + strand at 1810.030 kb on + strand at 1810.031 kb on - strand at 1810.046 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,807,649 + pepD OKFHMN_09700 0.42 +0.2 1,807,875 + pepD OKFHMN_09700 0.58 +0.7 1,807,879 + pepD OKFHMN_09700 0.58 -0.0 1,807,879 + pepD OKFHMN_09700 0.58 -0.5 1,807,880 - pepD OKFHMN_09700 0.58 -0.0 1,807,992 + pepD OKFHMN_09700 0.66 +0.6 1,807,992 + pepD OKFHMN_09700 0.66 -0.1 1,807,993 - pepD OKFHMN_09700 0.66 -1.4 1,808,105 + pepD OKFHMN_09700 0.74 +0.5 1,808,106 - pepD OKFHMN_09700 0.74 +0.4 1,808,153 - pepD OKFHMN_09700 0.77 -2.4 1,808,218 + pepD OKFHMN_09700 0.81 -1.3 1,808,219 - pepD OKFHMN_09700 0.81 -0.5 1,808,241 + pepD OKFHMN_09700 0.83 -0.1 1,808,241 + pepD OKFHMN_09700 0.83 -1.7 1,808,276 - pepD OKFHMN_09700 0.85 -2.1 1,808,313 + pepD OKFHMN_09700 0.88 +0.4 1,808,435 + -0.3 1,808,436 - -1.5 1,808,444 - -3.1 1,808,548 + -0.2 1,808,549 - -0.0 1,809,127 + ykfJ OKFHMN_09710 0.42 -2.5 1,809,182 - ykfJ OKFHMN_09710 0.62 +0.5 1,809,182 - ykfJ OKFHMN_09710 0.62 +0.2 1,809,232 - ykfJ OKFHMN_09710 0.81 +0.4 1,809,260 + -1.1 1,809,261 - -1.4 1,809,272 + -0.0 1,809,273 - +0.1 1,809,273 - -0.3 1,809,284 + +0.6 1,809,296 - +1.0 1,809,312 - +0.5 1,809,352 - -0.6 1,809,409 + +0.6 1,809,569 - -0.4 1,809,606 - +0.6 1,809,804 + yafP OKFHMN_09715 0.57 -0.6 1,809,811 - yafP OKFHMN_09715 0.59 -0.7 1,809,813 - yafP OKFHMN_09715 0.59 -0.6 1,810,024 + +0.1 1,810,030 + -0.2 1,810,030 + +0.2 1,810,031 - +0.6 1,810,046 + +0.4
Or see this region's nucleotide sequence