Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aRA1 and OKFHMN_09155 are separated by 192 nucleotides OKFHMN_09155 and lysR are separated by 287 nucleotides lysR and frsA are separated by 156 nucleotides
OKFHMN_09150: aRA1 - Aldo/keto reductase, related to diketogulonate reductase, at 1,719,638 to 1,720,213
aRA1
OKFHMN_09155: OKFHMN_09155 - hypothetical protein, at 1,720,406 to 1,720,762
_09155
OKFHMN_09160: lysR - LysR family transcriptional regulator, at 1,721,050 to 1,721,976
lysR
OKFHMN_09165: frsA - alpha/beta hydrolase, at 1,722,133 to 1,723,053
frsA
Position (kb) 1721
1722 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1720.082 kb on - strand, within aRA1 at 1720.102 kb on - strand, within aRA1 at 1720.137 kb on + strand, within aRA1 at 1720.138 kb on - strand, within aRA1 at 1720.219 kb on + strand at 1720.228 kb on - strand at 1720.328 kb on - strand at 1720.328 kb on - strand at 1720.356 kb on - strand at 1720.484 kb on + strand, within OKFHMN_09155 at 1720.484 kb on + strand, within OKFHMN_09155 at 1720.604 kb on + strand, within OKFHMN_09155 at 1720.978 kb on - strand at 1721.140 kb on - strand at 1721.186 kb on - strand, within lysR at 1721.505 kb on + strand, within lysR at 1721.533 kb on + strand, within lysR at 1721.566 kb on - strand, within lysR at 1721.594 kb on - strand, within lysR at 1721.617 kb on - strand, within lysR at 1721.796 kb on + strand, within lysR at 1721.830 kb on - strand, within lysR at 1721.878 kb on - strand, within lysR at 1722.039 kb on + strand at 1722.040 kb on - strand at 1722.170 kb on + strand at 1722.170 kb on + strand at 1722.171 kb on - strand at 1722.281 kb on - strand, within frsA at 1722.416 kb on - strand, within frsA at 1722.428 kb on + strand, within frsA at 1722.459 kb on - strand, within frsA at 1722.520 kb on - strand, within frsA at 1722.525 kb on + strand, within frsA at 1722.526 kb on - strand, within frsA at 1722.526 kb on - strand, within frsA at 1722.562 kb on - strand, within frsA at 1722.576 kb on + strand, within frsA at 1722.577 kb on - strand, within frsA at 1722.666 kb on - strand, within frsA at 1722.713 kb on + strand, within frsA at 1722.713 kb on + strand, within frsA at 1722.714 kb on - strand, within frsA at 1722.736 kb on + strand, within frsA at 1722.766 kb on - strand, within frsA at 1722.857 kb on + strand, within frsA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,720,082 - aRA1 OKFHMN_09150 0.77 -0.2 1,720,102 - aRA1 OKFHMN_09150 0.81 +0.4 1,720,137 + aRA1 OKFHMN_09150 0.87 -3.1 1,720,138 - aRA1 OKFHMN_09150 0.87 +0.2 1,720,219 + +1.1 1,720,228 - +1.3 1,720,328 - +0.2 1,720,328 - +0.1 1,720,356 - +0.1 1,720,484 + OKFHMN_09155 0.22 +0.1 1,720,484 + OKFHMN_09155 0.22 +1.0 1,720,604 + OKFHMN_09155 0.55 +1.7 1,720,978 - -4.1 1,721,140 - +0.1 1,721,186 - lysR OKFHMN_09160 0.15 -0.8 1,721,505 + lysR OKFHMN_09160 0.49 +1.0 1,721,533 + lysR OKFHMN_09160 0.52 -0.5 1,721,566 - lysR OKFHMN_09160 0.56 +0.1 1,721,594 - lysR OKFHMN_09160 0.59 -0.6 1,721,617 - lysR OKFHMN_09160 0.61 -0.0 1,721,796 + lysR OKFHMN_09160 0.80 -0.2 1,721,830 - lysR OKFHMN_09160 0.84 -0.0 1,721,878 - lysR OKFHMN_09160 0.89 -0.3 1,722,039 + -0.2 1,722,040 - +0.2 1,722,170 + -1.0 1,722,170 + -1.4 1,722,171 - +0.2 1,722,281 - frsA OKFHMN_09165 0.16 +0.0 1,722,416 - frsA OKFHMN_09165 0.31 +0.6 1,722,428 + frsA OKFHMN_09165 0.32 -0.2 1,722,459 - frsA OKFHMN_09165 0.35 -3.0 1,722,520 - frsA OKFHMN_09165 0.42 +0.2 1,722,525 + frsA OKFHMN_09165 0.43 -0.0 1,722,526 - frsA OKFHMN_09165 0.43 -2.4 1,722,526 - frsA OKFHMN_09165 0.43 -2.6 1,722,562 - frsA OKFHMN_09165 0.47 -0.5 1,722,576 + frsA OKFHMN_09165 0.48 -0.2 1,722,577 - frsA OKFHMN_09165 0.48 -2.6 1,722,666 - frsA OKFHMN_09165 0.58 +0.4 1,722,713 + frsA OKFHMN_09165 0.63 -1.5 1,722,713 + frsA OKFHMN_09165 0.63 -2.6 1,722,714 - frsA OKFHMN_09165 0.63 +1.7 1,722,736 + frsA OKFHMN_09165 0.65 +0.9 1,722,766 - frsA OKFHMN_09165 0.69 -0.8 1,722,857 + frsA OKFHMN_09165 0.79 -0.9
Or see this region's nucleotide sequence