Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpE and OKFHMN_08890 are separated by 39 nucleotides OKFHMN_08890 and prpC are separated by 33 nucleotides
OKFHMN_08885: prpE - propionate--CoA ligase, at 1,661,997 to 1,663,883
prpE
OKFHMN_08890: OKFHMN_08890 - bifunctional 2-methylcitrate dehydratase/aconitate hydratase, at 1,663,923 to 1,665,374
_08890
OKFHMN_08895: prpC - 2-methylcitrate synthase, at 1,665,408 to 1,666,577
prpC
Position (kb)
1663
1664
1665
1666 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1662.957 kb on + strand, within prpE at 1663.012 kb on + strand, within prpE at 1663.013 kb on - strand, within prpE at 1663.338 kb on + strand, within prpE at 1663.421 kb on + strand, within prpE at 1663.514 kb on + strand, within prpE at 1663.515 kb on - strand, within prpE at 1663.518 kb on - strand, within prpE at 1663.518 kb on - strand, within prpE at 1663.543 kb on + strand, within prpE at 1663.543 kb on + strand, within prpE at 1663.543 kb on + strand, within prpE at 1663.544 kb on - strand, within prpE at 1663.606 kb on - strand, within prpE at 1663.791 kb on - strand at 1663.850 kb on + strand at 1663.883 kb on + strand at 1663.883 kb on + strand at 1663.883 kb on + strand at 1663.883 kb on + strand at 1663.884 kb on - strand at 1663.904 kb on - strand at 1663.910 kb on + strand at 1663.921 kb on - strand at 1663.924 kb on + strand at 1663.924 kb on + strand at 1663.934 kb on - strand at 1663.945 kb on + strand at 1663.946 kb on - strand at 1664.051 kb on + strand at 1664.202 kb on - strand, within OKFHMN_08890 at 1664.205 kb on + strand, within OKFHMN_08890 at 1664.326 kb on + strand, within OKFHMN_08890 at 1664.548 kb on + strand, within OKFHMN_08890 at 1664.602 kb on + strand, within OKFHMN_08890 at 1665.161 kb on + strand, within OKFHMN_08890 at 1665.165 kb on + strand, within OKFHMN_08890 at 1665.259 kb on + strand at 1665.259 kb on + strand at 1665.298 kb on + strand at 1665.310 kb on + strand at 1665.311 kb on - strand at 1665.390 kb on - strand at 1665.584 kb on - strand, within prpC at 1665.584 kb on - strand, within prpC at 1665.584 kb on - strand, within prpC at 1665.667 kb on + strand, within prpC at 1665.668 kb on - strand, within prpC at 1665.668 kb on - strand, within prpC at 1665.749 kb on - strand, within prpC at 1665.919 kb on + strand, within prpC at 1665.920 kb on - strand, within prpC at 1665.923 kb on + strand, within prpC at 1665.923 kb on + strand, within prpC at 1665.923 kb on + strand, within prpC at 1666.005 kb on - strand, within prpC at 1666.121 kb on - strand, within prpC at 1666.126 kb on + strand, within prpC at 1666.132 kb on + strand, within prpC at 1666.149 kb on - strand, within prpC at 1666.176 kb on - strand, within prpC at 1666.196 kb on + strand, within prpC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,662,957 + prpE OKFHMN_08885 0.51 +0.8 1,663,012 + prpE OKFHMN_08885 0.54 -0.2 1,663,013 - prpE OKFHMN_08885 0.54 -0.7 1,663,338 + prpE OKFHMN_08885 0.71 +0.1 1,663,421 + prpE OKFHMN_08885 0.75 +0.3 1,663,514 + prpE OKFHMN_08885 0.80 -0.2 1,663,515 - prpE OKFHMN_08885 0.80 -0.1 1,663,518 - prpE OKFHMN_08885 0.81 +0.2 1,663,518 - prpE OKFHMN_08885 0.81 -0.7 1,663,543 + prpE OKFHMN_08885 0.82 +0.9 1,663,543 + prpE OKFHMN_08885 0.82 -4.0 1,663,543 + prpE OKFHMN_08885 0.82 -0.7 1,663,544 - prpE OKFHMN_08885 0.82 +0.3 1,663,606 - prpE OKFHMN_08885 0.85 -0.0 1,663,791 - -1.6 1,663,850 + +0.1 1,663,883 + -0.2 1,663,883 + +0.2 1,663,883 + -0.0 1,663,883 + -0.4 1,663,884 - +0.3 1,663,904 - +0.1 1,663,910 + -0.1 1,663,921 - -3.8 1,663,924 + -2.1 1,663,924 + +0.8 1,663,934 - +1.3 1,663,945 + -2.3 1,663,946 - -1.3 1,664,051 + -0.8 1,664,202 - OKFHMN_08890 0.19 +1.2 1,664,205 + OKFHMN_08890 0.19 -1.1 1,664,326 + OKFHMN_08890 0.28 -0.9 1,664,548 + OKFHMN_08890 0.43 +0.7 1,664,602 + OKFHMN_08890 0.47 +0.3 1,665,161 + OKFHMN_08890 0.85 -0.3 1,665,165 + OKFHMN_08890 0.86 +0.3 1,665,259 + -1.6 1,665,259 + +0.6 1,665,298 + +0.2 1,665,310 + +0.6 1,665,311 - +1.1 1,665,390 - +0.2 1,665,584 - prpC OKFHMN_08895 0.15 +1.2 1,665,584 - prpC OKFHMN_08895 0.15 -0.1 1,665,584 - prpC OKFHMN_08895 0.15 -0.7 1,665,667 + prpC OKFHMN_08895 0.22 -0.1 1,665,668 - prpC OKFHMN_08895 0.22 -0.0 1,665,668 - prpC OKFHMN_08895 0.22 -1.1 1,665,749 - prpC OKFHMN_08895 0.29 -0.4 1,665,919 + prpC OKFHMN_08895 0.44 +0.5 1,665,920 - prpC OKFHMN_08895 0.44 -0.0 1,665,923 + prpC OKFHMN_08895 0.44 +0.9 1,665,923 + prpC OKFHMN_08895 0.44 +0.4 1,665,923 + prpC OKFHMN_08895 0.44 +1.7 1,666,005 - prpC OKFHMN_08895 0.51 +0.2 1,666,121 - prpC OKFHMN_08895 0.61 -0.3 1,666,126 + prpC OKFHMN_08895 0.61 -0.1 1,666,132 + prpC OKFHMN_08895 0.62 +0.8 1,666,149 - prpC OKFHMN_08895 0.63 +0.5 1,666,176 - prpC OKFHMN_08895 0.66 -1.5 1,666,196 + prpC OKFHMN_08895 0.67 +0.3
Or see this region's nucleotide sequence