Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuE and ssuA overlap by 8 nucleotides ssuA and ssuD overlap by 4 nucleotides ssuD and ssuC are separated by 11 nucleotides ssuC and ssuB overlap by 4 nucleotides
OKFHMN_05340: ssuE - NADPH-dependent FMN reductase, at 889,184 to 889,759
ssuE
OKFHMN_05345: ssuA - aliphatic sulfonate ABC transporter substrate-binding protein SsuA, at 889,752 to 890,711
ssuA
OKFHMN_05350: ssuD - FMNH2-dependent alkanesulfonate monooxygenase, at 890,708 to 891,853
ssuD
OKFHMN_05355: ssuC - aliphatic sulfonate ABC transporter permease SsuC, at 891,865 to 892,656
ssuC
OKFHMN_05360: ssuB - aliphatic sulfonates ABC transporter ATP-binding protein, at 892,653 to 893,420
ssuB
Position (kb) 890
891
892 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 889.767 kb on - strand at 889.837 kb on + strand at 889.837 kb on + strand at 889.842 kb on - strand at 889.842 kb on - strand at 889.844 kb on - strand at 889.868 kb on + strand, within ssuA at 890.211 kb on - strand, within ssuA at 890.236 kb on - strand, within ssuA at 890.308 kb on - strand, within ssuA at 890.404 kb on - strand, within ssuA at 890.404 kb on - strand, within ssuA at 890.437 kb on + strand, within ssuA at 890.437 kb on + strand, within ssuA at 890.544 kb on + strand, within ssuA at 890.545 kb on - strand, within ssuA at 890.602 kb on + strand, within ssuA at 890.719 kb on + strand at 890.719 kb on + strand at 890.838 kb on - strand, within ssuD at 890.839 kb on + strand, within ssuD at 891.170 kb on - strand, within ssuD at 891.320 kb on - strand, within ssuD at 891.534 kb on + strand, within ssuD at 891.539 kb on - strand, within ssuD at 891.851 kb on + strand at 892.346 kb on - strand, within ssuC at 892.407 kb on + strand, within ssuC at 892.407 kb on + strand, within ssuC at 892.548 kb on + strand, within ssuC at 892.652 kb on + strand at 892.652 kb on + strand at 892.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 889,767 - +1.0 889,837 + +0.5 889,837 + +0.4 889,842 - +1.6 889,842 - -0.2 889,844 - -1.0 889,868 + ssuA OKFHMN_05345 0.12 +0.6 890,211 - ssuA OKFHMN_05345 0.48 -1.5 890,236 - ssuA OKFHMN_05345 0.50 -1.7 890,308 - ssuA OKFHMN_05345 0.58 +0.3 890,404 - ssuA OKFHMN_05345 0.68 -1.1 890,404 - ssuA OKFHMN_05345 0.68 +0.4 890,437 + ssuA OKFHMN_05345 0.71 -3.9 890,437 + ssuA OKFHMN_05345 0.71 +0.3 890,544 + ssuA OKFHMN_05345 0.82 +1.0 890,545 - ssuA OKFHMN_05345 0.83 +0.3 890,602 + ssuA OKFHMN_05345 0.89 -4.6 890,719 + +1.7 890,719 + +0.2 890,838 - ssuD OKFHMN_05350 0.11 +0.5 890,839 + ssuD OKFHMN_05350 0.11 +0.5 891,170 - ssuD OKFHMN_05350 0.40 -0.0 891,320 - ssuD OKFHMN_05350 0.53 +0.6 891,534 + ssuD OKFHMN_05350 0.72 +0.4 891,539 - ssuD OKFHMN_05350 0.73 +0.1 891,851 + +0.2 892,346 - ssuC OKFHMN_05355 0.61 -1.0 892,407 + ssuC OKFHMN_05355 0.68 +0.0 892,407 + ssuC OKFHMN_05355 0.68 +0.1 892,548 + ssuC OKFHMN_05355 0.86 -0.3 892,652 + +0.1 892,652 + +1.8 892,702 + -1.1
Or see this region's nucleotide sequence