Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_04960 and OKFHMN_04965 are separated by 255 nucleotides OKFHMN_04965 and OKFHMN_04970 are separated by 159 nucleotides OKFHMN_04970 and OKFHMN_04975 are separated by 220 nucleotides
OKFHMN_04960: OKFHMN_04960 - holin, at 829,794 to 830,000
_04960
OKFHMN_04965: OKFHMN_04965 - hypothetical protein, at 830,256 to 830,528
_04965
OKFHMN_04970: OKFHMN_04970 - lysozyme, at 830,688 to 831,221
_04970
OKFHMN_04975: OKFHMN_04975 - hypothetical protein, at 831,442 to 831,555
_04975
OKFHMN_04980: OKFHMN_04980 - lysis protein, at 831,500 to 832,024
_04980
Position (kb) 830
831 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 829.565 kb on + strand at 829.565 kb on + strand at 829.999 kb on - strand at 830.016 kb on + strand at 830.341 kb on - strand, within OKFHMN_04965 at 830.484 kb on - strand, within OKFHMN_04965 at 830.530 kb on - strand at 830.572 kb on + strand at 830.572 kb on + strand at 830.573 kb on - strand at 830.653 kb on + strand at 830.877 kb on - strand, within OKFHMN_04970 at 831.219 kb on + strand at 831.220 kb on - strand at 831.441 kb on + strand at 831.442 kb on - strand at 831.510 kb on + strand at 831.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 829,565 + +0.0 829,565 + -0.7 829,999 - -0.7 830,016 + -1.6 830,341 - OKFHMN_04965 0.31 +1.1 830,484 - OKFHMN_04965 0.84 -1.9 830,530 - -0.4 830,572 + -0.7 830,572 + +0.2 830,573 - -1.2 830,653 + -1.8 830,877 - OKFHMN_04970 0.35 -0.5 831,219 + -1.8 831,220 - +0.5 831,441 + +0.6 831,442 - -3.7 831,510 + -0.8 831,511 - -0.1
Or see this region's nucleotide sequence