Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt terW and OKFHMN_04190 are separated by 260 nucleotides OKFHMN_04190 and tnpB overlap by 14 nucleotides tnpB and OKFHMN_04200 are separated by 81 nucleotides OKFHMN_04200 and OKFHMN_04205 are separated by 3 nucleotides
OKFHMN_04185: terW - tellurium resistance protein TerW, at 671,497 to 671,964
terW
OKFHMN_04190: OKFHMN_04190 - IS66 family insertion sequence hypothetical protein, at 672,225 to 672,554
_04190
OKFHMN_04195: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 672,541 to 672,882
tnpB
OKFHMN_04200: OKFHMN_04200 - IS66 family transposase, at 672,964 to 673,821
_04200
OKFHMN_04205: OKFHMN_04205 - IS66-like element IS682 family transposase, at 673,825 to 675,438
_04205
Position (kb) 672
673
674 Strain fitness (log2 ratio) -2
-1
0
1
2 at 672.182 kb on + strand at 672.210 kb on + strand at 672.555 kb on + strand at 672.560 kb on + strand at 672.616 kb on + strand, within tnpB at 672.616 kb on + strand, within tnpB at 672.617 kb on - strand, within tnpB at 672.617 kb on - strand, within tnpB at 672.693 kb on - strand, within tnpB at 673.417 kb on + strand, within OKFHMN_04200 at 673.483 kb on - strand, within OKFHMN_04200 at 673.698 kb on + strand, within OKFHMN_04200 at 673.777 kb on + strand at 674.062 kb on - strand, within OKFHMN_04205 at 674.094 kb on - strand, within OKFHMN_04205 at 674.731 kb on + strand, within OKFHMN_04205
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 672,182 + -0.8 672,210 + +0.2 672,555 + +0.1 672,560 + +1.1 672,616 + tnpB OKFHMN_04195 0.22 +1.5 672,616 + tnpB OKFHMN_04195 0.22 +2.1 672,617 - tnpB OKFHMN_04195 0.22 -1.4 672,617 - tnpB OKFHMN_04195 0.22 +1.8 672,693 - tnpB OKFHMN_04195 0.44 +0.6 673,417 + OKFHMN_04200 0.53 -2.0 673,483 - OKFHMN_04200 0.60 -1.6 673,698 + OKFHMN_04200 0.86 -0.1 673,777 + +0.5 674,062 - OKFHMN_04205 0.15 -0.4 674,094 - OKFHMN_04205 0.17 +0.8 674,731 + OKFHMN_04205 0.56 -1.2
Or see this region's nucleotide sequence