Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03835

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcsgC and csgA are separated by 58 nucleotidescsgA and csgB are separated by 40 nucleotidescsgB and cgsD are separated by 753 nucleotides OKFHMN_03825: csgC - curli assembly protein CsgC, at 611,672 to 612,004 csgC OKFHMN_03830: csgA - curlin major subunit CsgA, at 612,063 to 612,521 csgA OKFHMN_03835: csgB - curlin minor subunit CsgB, at 612,562 to 613,017 csgB OKFHMN_03845: cgsD - transcriptional regulator CsgD, at 613,771 to 614,421 cgsD Position (kb) 612 613 614Strain fitness (log2 ratio) -2 -1 0 1 2at 611.877 kb on - strand, within csgCat 611.931 kb on + strand, within csgCat 611.931 kb on + strand, within csgCat 612.004 kb on + strandat 612.062 kb on - strandat 612.062 kb on - strandat 612.064 kb on + strandat 612.067 kb on + strandat 612.183 kb on - strand, within csgAat 612.224 kb on + strand, within csgAat 612.312 kb on - strand, within csgAat 612.312 kb on - strand, within csgAat 612.546 kb on - strandat 612.608 kb on - strand, within csgBat 612.640 kb on - strand, within csgBat 612.742 kb on - strand, within csgBat 612.744 kb on - strand, within csgBat 612.755 kb on + strand, within csgBat 612.901 kb on - strand, within csgBat 613.176 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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611,877 - csgC OKFHMN_03825 0.62 +0.2
611,931 + csgC OKFHMN_03825 0.78 -2.5
611,931 + csgC OKFHMN_03825 0.78 -0.4
612,004 + -0.7
612,062 - -0.7
612,062 - -0.5
612,064 + -2.2
612,067 + +1.2
612,183 - csgA OKFHMN_03830 0.26 +0.2
612,224 + csgA OKFHMN_03830 0.35 -1.4
612,312 - csgA OKFHMN_03830 0.54 -0.1
612,312 - csgA OKFHMN_03830 0.54 -1.3
612,546 - +0.3
612,608 - csgB OKFHMN_03835 0.10 +2.2
612,640 - csgB OKFHMN_03835 0.17 -0.1
612,742 - csgB OKFHMN_03835 0.39 +0.9
612,744 - csgB OKFHMN_03835 0.40 +0.8
612,755 + csgB OKFHMN_03835 0.42 +1.7
612,901 - csgB OKFHMN_03835 0.74 -0.4
613,176 - -1.5

Or see this region's nucleotide sequence