Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnpB and tnpB overlap by 4 nucleotides tnpB and OKFHMN_02725 are separated by 49 nucleotides OKFHMN_02725 and OKFHMN_02730 are separated by 284 nucleotides OKFHMN_02730 and iS285 are separated by 29 nucleotides
OKFHMN_02715: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 435,398 to 435,778
tnpB
OKFHMN_02720: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 435,775 to 436,122
tnpB
OKFHMN_02725: OKFHMN_02725 - IS66-like element ISEc8 family transposase, at 436,172 to 437,710
_02725
OKFHMN_02730: OKFHMN_02730 - Mutator family transposase, at 437,995 to 438,342
_02730
OKFHMN_02735: iS285 - Transposase (or an inactivated derivative), at 438,372 to 438,629
iS285
Position (kb) 436
437
438 Strain fitness (log2 ratio) -2
-1
0
1 at 435.308 kb on - strand at 438.002 kb on + strand at 438.179 kb on + strand, within OKFHMN_02730 at 438.341 kb on - strand at 438.369 kb on - strand at 438.417 kb on + strand, within iS285 at 438.418 kb on - strand, within iS285 at 438.591 kb on - strand, within iS285 at 438.689 kb on - strand at 438.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 435,308 - -0.1 438,002 + -0.0 438,179 + OKFHMN_02730 0.53 +0.3 438,341 - +0.1 438,369 - -2.4 438,417 + iS285 OKFHMN_02735 0.17 +0.1 438,418 - iS285 OKFHMN_02735 0.18 -0.5 438,591 - iS285 OKFHMN_02735 0.85 +0.3 438,689 - +0.5 438,705 - +0.1
Or see this region's nucleotide sequence