Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02685

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpA and yciG are separated by 380 nucleotidesyciG and yciF are separated by 85 nucleotidesyciF and yciE are separated by 45 nucleotides OKFHMN_02675: trpA - tryptophan synthase subunit alpha, at 426,673 to 427,479 trpA OKFHMN_02680: yciG - Uncharacterized protein YciG, at 427,860 to 428,039 yciG OKFHMN_02685: yciF - Protein YciF, at 428,125 to 428,625 yciF OKFHMN_02690: yciE - Protein YciE, at 428,671 to 429,177 yciE Position (kb) 428 429Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 427.190 kb on - strand, within trpAat 427.280 kb on - strand, within trpAat 428.068 kb on + strandat 429.488 kb on + strandat 429.503 kb on - strandat 429.555 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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427,190 - trpA OKFHMN_02675 0.64 -1.5
427,280 - trpA OKFHMN_02675 0.75 -4.7
428,068 + +0.4
429,488 + +0.8
429,503 - -3.3
429,555 + -0.5

Or see this region's nucleotide sequence