Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02650

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciV and trpL are separated by 137 nucleotidestrpL and trpE are separated by 91 nucleotides OKFHMN_02645: yciV - 5'-3' exoribonuclease, at 419,794 to 420,675 yciV OKFHMN_02650: trpL - trp operon leader peptide, at 420,813 to 420,857 trpL OKFHMN_02655: trpE - anthranilate synthase component I, at 420,949 to 422,511 trpE Position (kb) 420 421Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 419.968 kb on - strand, within yciVat 420.089 kb on - strand, within yciVat 420.141 kb on + strand, within yciVat 420.308 kb on - strand, within yciVat 420.308 kb on - strand, within yciVat 420.427 kb on - strand, within yciVat 420.829 kb on + strand, within trpLat 420.882 kb on + strandat 421.792 kb on + strand, within trpEat 421.809 kb on + strand, within trpEat 421.822 kb on + strand, within trpEat 421.823 kb on - strand, within trpE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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419,968 - yciV OKFHMN_02645 0.20 +0.1
420,089 - yciV OKFHMN_02645 0.33 +1.0
420,141 + yciV OKFHMN_02645 0.39 -1.9
420,308 - yciV OKFHMN_02645 0.58 +1.7
420,308 - yciV OKFHMN_02645 0.58 +2.2
420,427 - yciV OKFHMN_02645 0.72 -1.0
420,829 + trpL OKFHMN_02650 0.36 +0.2
420,882 + -3.1
421,792 + trpE OKFHMN_02655 0.54 -2.7
421,809 + trpE OKFHMN_02655 0.55 -2.7
421,822 + trpE OKFHMN_02655 0.56 -4.4
421,823 - trpE OKFHMN_02655 0.56 -2.6

Or see this region's nucleotide sequence