Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt abgB and OKFHMN_02215 are separated by 36 nucleotides OKFHMN_02215 and tnp-IS3 are separated by 25 nucleotides tnp-IS3 and tra5 overlap by 1 nucleotides
OKFHMN_02210: abgB - p-aminobenzoyl-glutamate hydrolase subunit AbgB, at 332,426 to 333,871
abgB
OKFHMN_02215: OKFHMN_02215 - Pump protein disrupted by IS629, at 333,908 to 334,705
_02215
OKFHMN_02220: tnp-IS3 - IS3 family transposase, at 334,731 to 335,057
tnp-IS3
OKFHMN_02225: tra5 - IS3 family transposase, at 335,057 to 335,707
tra5
Position (kb) 333
334
335 Strain fitness (log2 ratio) -2
-1
0
1
2 at 333.107 kb on - strand, within abgB at 333.107 kb on - strand, within abgB at 333.109 kb on + strand, within abgB at 333.127 kb on - strand, within abgB at 333.127 kb on - strand, within abgB at 333.146 kb on - strand, within abgB at 333.232 kb on + strand, within abgB at 333.324 kb on - strand, within abgB at 333.356 kb on + strand, within abgB at 333.357 kb on - strand, within abgB at 333.466 kb on + strand, within abgB at 333.470 kb on - strand, within abgB at 333.653 kb on - strand, within abgB at 333.665 kb on - strand, within abgB at 333.665 kb on - strand, within abgB at 333.698 kb on - strand, within abgB at 333.698 kb on - strand, within abgB at 333.775 kb on - strand at 333.913 kb on + strand at 333.914 kb on - strand at 333.914 kb on - strand at 333.953 kb on + strand at 334.007 kb on - strand, within OKFHMN_02215 at 334.011 kb on - strand, within OKFHMN_02215 at 334.233 kb on + strand, within OKFHMN_02215 at 334.289 kb on + strand, within OKFHMN_02215 at 334.289 kb on + strand, within OKFHMN_02215 at 334.289 kb on + strand, within OKFHMN_02215 at 334.290 kb on - strand, within OKFHMN_02215 at 334.292 kb on - strand, within OKFHMN_02215 at 334.543 kb on + strand, within OKFHMN_02215
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 333,107 - abgB OKFHMN_02210 0.47 +2.7 333,107 - abgB OKFHMN_02210 0.47 +0.3 333,109 + abgB OKFHMN_02210 0.47 +0.7 333,127 - abgB OKFHMN_02210 0.48 -0.1 333,127 - abgB OKFHMN_02210 0.48 +0.3 333,146 - abgB OKFHMN_02210 0.50 -1.4 333,232 + abgB OKFHMN_02210 0.56 -0.2 333,324 - abgB OKFHMN_02210 0.62 -0.7 333,356 + abgB OKFHMN_02210 0.64 +0.1 333,357 - abgB OKFHMN_02210 0.64 -0.1 333,466 + abgB OKFHMN_02210 0.72 -2.3 333,470 - abgB OKFHMN_02210 0.72 +0.1 333,653 - abgB OKFHMN_02210 0.85 +0.5 333,665 - abgB OKFHMN_02210 0.86 -2.1 333,665 - abgB OKFHMN_02210 0.86 +0.2 333,698 - abgB OKFHMN_02210 0.88 -2.3 333,698 - abgB OKFHMN_02210 0.88 -0.8 333,775 - +1.3 333,913 + -2.5 333,914 - +0.2 333,914 - +0.5 333,953 + +0.5 334,007 - OKFHMN_02215 0.12 +0.2 334,011 - OKFHMN_02215 0.13 +0.2 334,233 + OKFHMN_02215 0.41 -1.1 334,289 + OKFHMN_02215 0.48 -1.9 334,289 + OKFHMN_02215 0.48 +0.9 334,289 + OKFHMN_02215 0.48 +0.7 334,290 - OKFHMN_02215 0.48 -0.3 334,292 - OKFHMN_02215 0.48 -1.0 334,543 + OKFHMN_02215 0.80 +1.7
Or see this region's nucleotide sequence