Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_01775 and ydbL are separated by 413 nucleotides ydbL and OKFHMN_01785 are separated by 7 nucleotides OKFHMN_01785 and ynbE overlap by 4 nucleotides ynbE and ldhA are separated by 207 nucleotides
OKFHMN_01775: OKFHMN_01775 - surface-exposed virulence protein BigA, at 263,912 to 266,791
_01775
OKFHMN_01780: ydbL - DUF1318 domain-containing protein, at 267,205 to 267,528
ydbL
OKFHMN_01785: OKFHMN_01785 - YnbE family lipoprotein, at 267,536 to 267,721
_01785
OKFHMN_01790: ynbE - YdbH family protein, at 267,718 to 270,357
ynbE
OKFHMN_01795: ldhA - D-lactate dehydrogenase, at 270,565 to 271,554
ldhA
Position (kb) 267
268
269
270
271 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 266.810 kb on - strand at 267.978 kb on - strand at 268.152 kb on + strand, within ynbE at 268.271 kb on - strand, within ynbE at 268.372 kb on - strand, within ynbE at 268.569 kb on - strand, within ynbE at 268.721 kb on - strand, within ynbE at 268.732 kb on - strand, within ynbE at 269.049 kb on - strand, within ynbE at 269.188 kb on + strand, within ynbE at 269.189 kb on - strand, within ynbE at 269.728 kb on - strand, within ynbE at 269.741 kb on - strand, within ynbE at 270.344 kb on + strand at 270.456 kb on + strand at 270.456 kb on + strand at 270.456 kb on + strand at 270.532 kb on + strand at 270.988 kb on - strand, within ldhA at 270.991 kb on + strand, within ldhA at 270.992 kb on - strand, within ldhA at 271.065 kb on + strand, within ldhA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 266,810 - -3.2 267,978 - -0.4 268,152 + ynbE OKFHMN_01790 0.16 +0.5 268,271 - ynbE OKFHMN_01790 0.21 +2.1 268,372 - ynbE OKFHMN_01790 0.25 -0.7 268,569 - ynbE OKFHMN_01790 0.32 +1.0 268,721 - ynbE OKFHMN_01790 0.38 +0.3 268,732 - ynbE OKFHMN_01790 0.38 +0.0 269,049 - ynbE OKFHMN_01790 0.50 -0.9 269,188 + ynbE OKFHMN_01790 0.56 +1.1 269,189 - ynbE OKFHMN_01790 0.56 -0.0 269,728 - ynbE OKFHMN_01790 0.76 +0.0 269,741 - ynbE OKFHMN_01790 0.77 +0.4 270,344 + -1.4 270,456 + -0.5 270,456 + -0.2 270,456 + -1.6 270,532 + +0.3 270,988 - ldhA OKFHMN_01795 0.43 -1.6 270,991 + ldhA OKFHMN_01795 0.43 -0.1 270,992 - ldhA OKFHMN_01795 0.43 -1.2 271,065 + ldhA OKFHMN_01795 0.51 -2.5
Or see this region's nucleotide sequence