Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxI and OKFHMN_01770 are separated by 219 nucleotides OKFHMN_01770 and OKFHMN_01775 are separated by 219 nucleotides
OKFHMN_01765: pdxI - pyridoxine 4-dehydrogenase, at 260,153 to 261,013
pdxI
OKFHMN_01770: OKFHMN_01770 - BigB-like protein, at 261,233 to 263,692
_01770
OKFHMN_01775: OKFHMN_01775 - surface-exposed virulence protein BigA, at 263,912 to 266,791
_01775
Position (kb) 261
262
263
264 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.539 kb on + strand, within pdxI at 261.110 kb on + strand at 261.334 kb on - strand at 261.448 kb on - strand at 261.812 kb on - strand, within OKFHMN_01770 at 261.968 kb on - strand, within OKFHMN_01770 at 261.968 kb on - strand, within OKFHMN_01770 at 262.201 kb on - strand, within OKFHMN_01770 at 262.525 kb on + strand, within OKFHMN_01770 at 262.528 kb on - strand, within OKFHMN_01770 at 262.600 kb on - strand, within OKFHMN_01770 at 263.205 kb on - strand, within OKFHMN_01770 at 263.237 kb on - strand, within OKFHMN_01770 at 263.240 kb on + strand, within OKFHMN_01770 at 263.621 kb on - strand at 263.846 kb on + strand at 263.868 kb on - strand at 263.898 kb on + strand at 264.224 kb on + strand, within OKFHMN_01775 at 264.299 kb on - strand, within OKFHMN_01775 at 264.389 kb on + strand, within OKFHMN_01775 at 264.415 kb on - strand, within OKFHMN_01775 at 264.428 kb on - strand, within OKFHMN_01775 at 264.472 kb on + strand, within OKFHMN_01775 at 264.497 kb on - strand, within OKFHMN_01775
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 260,539 + pdxI OKFHMN_01765 0.45 -0.1 261,110 + -1.0 261,334 - +0.3 261,448 - +1.1 261,812 - OKFHMN_01770 0.24 -1.9 261,968 - OKFHMN_01770 0.30 +0.2 261,968 - OKFHMN_01770 0.30 -2.6 262,201 - OKFHMN_01770 0.39 +0.9 262,525 + OKFHMN_01770 0.53 +0.2 262,528 - OKFHMN_01770 0.53 +0.6 262,600 - OKFHMN_01770 0.56 -1.1 263,205 - OKFHMN_01770 0.80 +0.2 263,237 - OKFHMN_01770 0.81 -1.4 263,240 + OKFHMN_01770 0.82 +0.3 263,621 - +0.5 263,846 + +0.2 263,868 - +1.3 263,898 + -2.9 264,224 + OKFHMN_01775 0.11 -0.1 264,299 - OKFHMN_01775 0.13 +1.0 264,389 + OKFHMN_01775 0.17 +0.1 264,415 - OKFHMN_01775 0.17 +0.6 264,428 - OKFHMN_01775 0.18 -2.4 264,472 + OKFHMN_01775 0.19 -0.4 264,497 - OKFHMN_01775 0.20 +1.6
Or see this region's nucleotide sequence