Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01760

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynbB and ynbA overlap by 1 nucleotidesynbA and ydbD are separated by 170 nucleotidesydbD and pdxI are separated by 63 nucleotides OKFHMN_01750: ynbB - Uncharacterized protein YnbB, at 256,111 to 257,007 ynbB OKFHMN_01755: ynbA - Inner membrane protein YnbA, at 257,007 to 257,612 ynbA OKFHMN_01760: ydbD - Uncharacterized protein YdbD, at 257,783 to 260,089 ydbD OKFHMN_01765: pdxI - pyridoxine 4-dehydrogenase, at 260,153 to 261,013 pdxI Position (kb) 257 258 259 260 261Strain fitness (log2 ratio) -1 0 1 2at 257.254 kb on - strand, within ynbAat 258.236 kb on + strand, within ydbDat 258.927 kb on - strand, within ydbDat 259.071 kb on - strand, within ydbDat 259.071 kb on - strand, within ydbDat 260.539 kb on + strand, within pdxI

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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257,254 - ynbA OKFHMN_01755 0.41 +0.8
258,236 + ydbD OKFHMN_01760 0.20 +0.5
258,927 - ydbD OKFHMN_01760 0.50 -0.7
259,071 - ydbD OKFHMN_01760 0.56 +1.9
259,071 - ydbD OKFHMN_01760 0.56 -0.4
260,539 + pdxI OKFHMN_01765 0.45 -0.1

Or see this region's nucleotide sequence