Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01140

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneE and tam are separated by 3 nucleotidestam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotides OKFHMN_01135: yneE - UPF0187 protein YneE, at 115,662 to 116,576 yneE OKFHMN_01140: tam - trans-aconitate 2-methyltransferase, at 116,580 to 117,338 tam OKFHMN_01145: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 117,395 to 117,685 lsrG OKFHMN_01150: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 117,709 to 118,584 lsrF Position (kb) 116 117 118Strain fitness (log2 ratio) -2 -1 0 1 2at 116.226 kb on - strand, within yneEat 117.313 kb on + strandat 117.315 kb on + strandat 117.491 kb on - strand, within lsrGat 117.729 kb on - strandat 117.738 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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116,226 - yneE OKFHMN_01135 0.62 +0.1
117,313 + -0.9
117,315 + -1.2
117,491 - lsrG OKFHMN_01145 0.33 +2.2
117,729 - -0.3
117,738 - -2.5

Or see this region's nucleotide sequence