Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt repA2 and OKFHMN_00410 are separated by 239 nucleotides OKFHMN_00410 and OKFHMN_00415 are separated by 37 nucleotides OKFHMN_00415 and yncB are separated by 45 nucleotides yncB and OKFHMN_00430 are separated by 201 nucleotides OKFHMN_00430 and finO are separated by 136 nucleotides
OKFHMN_00405: repA2 - Replication regulatory protein repA2, at 86,042 to 86,296
repA2
OKFHMN_00410: OKFHMN_00410 - W0003, at 86,536 to 86,874
_00410
OKFHMN_00415: OKFHMN_00415 - transcriptional regulator, at 86,912 to 87,121
_00415
OKFHMN_00420: yncB - Uncharacterized endonuclease, at 87,167 to 87,628
yncB
OKFHMN_00430: OKFHMN_00430 - hypothetical protein, at 87,830 to 88,081
_00430
OKFHMN_00435: finO - Fertility inhibition protein, at 88,218 to 88,778
finO
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 86.188 kb on + strand, within repA2 at 86.201 kb on + strand, within repA2 at 86.244 kb on + strand, within repA2 at 86.245 kb on - strand, within repA2 at 86.311 kb on - strand at 86.314 kb on + strand at 86.314 kb on + strand at 86.315 kb on - strand at 86.343 kb on - strand at 86.414 kb on - strand at 86.423 kb on + strand at 86.434 kb on - strand at 86.445 kb on + strand at 86.445 kb on + strand at 86.445 kb on + strand at 86.446 kb on - strand at 86.446 kb on - strand at 86.541 kb on + strand at 86.542 kb on - strand at 86.640 kb on + strand, within OKFHMN_00410 at 86.641 kb on - strand, within OKFHMN_00410 at 86.699 kb on - strand, within OKFHMN_00410 at 86.715 kb on - strand, within OKFHMN_00410 at 86.755 kb on + strand, within OKFHMN_00410 at 86.828 kb on - strand, within OKFHMN_00410 at 87.279 kb on + strand, within yncB at 87.288 kb on + strand, within yncB at 87.289 kb on - strand, within yncB at 87.291 kb on - strand, within yncB at 87.316 kb on + strand, within yncB at 87.317 kb on - strand, within yncB at 87.454 kb on - strand, within yncB at 87.481 kb on + strand, within yncB at 87.482 kb on - strand, within yncB at 87.546 kb on - strand, within yncB at 87.569 kb on - strand, within yncB at 87.619 kb on - strand at 87.747 kb on + strand at 88.090 kb on - strand at 88.102 kb on + strand at 88.113 kb on + strand at 88.116 kb on + strand at 88.116 kb on + strand at 88.116 kb on + strand at 88.128 kb on + strand at 88.128 kb on + strand at 88.129 kb on - strand at 88.129 kb on - strand at 88.176 kb on - strand at 88.277 kb on + strand, within finO at 88.298 kb on - strand, within finO at 88.298 kb on - strand, within finO at 88.319 kb on + strand, within finO at 88.320 kb on - strand, within finO at 88.320 kb on - strand, within finO at 88.320 kb on - strand, within finO at 88.320 kb on - strand, within finO at 88.334 kb on - strand, within finO at 88.518 kb on + strand, within finO at 88.579 kb on + strand, within finO
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 86,188 + repA2 OKFHMN_00405 0.57 -0.9 86,201 + repA2 OKFHMN_00405 0.62 -1.1 86,244 + repA2 OKFHMN_00405 0.79 +0.2 86,245 - repA2 OKFHMN_00405 0.80 -0.8 86,311 - -1.7 86,314 + +2.1 86,314 + -2.7 86,315 - -2.7 86,343 - -0.8 86,414 - -0.1 86,423 + -0.1 86,434 - -2.1 86,445 + -0.3 86,445 + +1.1 86,445 + -0.6 86,446 - -0.2 86,446 - -1.4 86,541 + -0.1 86,542 - +0.4 86,640 + OKFHMN_00410 0.31 -0.0 86,641 - OKFHMN_00410 0.31 +0.4 86,699 - OKFHMN_00410 0.48 -0.4 86,715 - OKFHMN_00410 0.53 +1.7 86,755 + OKFHMN_00410 0.65 -3.1 86,828 - OKFHMN_00410 0.86 +0.6 87,279 + yncB OKFHMN_00420 0.24 -0.9 87,288 + yncB OKFHMN_00420 0.26 -0.0 87,289 - yncB OKFHMN_00420 0.26 -0.3 87,291 - yncB OKFHMN_00420 0.27 +0.5 87,316 + yncB OKFHMN_00420 0.32 -2.0 87,317 - yncB OKFHMN_00420 0.32 +0.4 87,454 - yncB OKFHMN_00420 0.62 +1.0 87,481 + yncB OKFHMN_00420 0.68 -0.1 87,482 - yncB OKFHMN_00420 0.68 +2.7 87,546 - yncB OKFHMN_00420 0.82 -0.1 87,569 - yncB OKFHMN_00420 0.87 +0.5 87,619 - +1.0 87,747 + -1.1 88,090 - +0.1 88,102 + -0.5 88,113 + +0.2 88,116 + +0.2 88,116 + +0.3 88,116 + -0.2 88,128 + -0.5 88,128 + -1.1 88,129 - -0.6 88,129 - +0.4 88,176 - +0.8 88,277 + finO OKFHMN_00435 0.11 +0.4 88,298 - finO OKFHMN_00435 0.14 +1.6 88,298 - finO OKFHMN_00435 0.14 +0.9 88,319 + finO OKFHMN_00435 0.18 +0.2 88,320 - finO OKFHMN_00435 0.18 +0.1 88,320 - finO OKFHMN_00435 0.18 +1.4 88,320 - finO OKFHMN_00435 0.18 +0.2 88,320 - finO OKFHMN_00435 0.18 +1.9 88,334 - finO OKFHMN_00435 0.21 +0.4 88,518 + finO OKFHMN_00435 0.53 -0.0 88,579 + finO OKFHMN_00435 0.64 -1.0
Or see this region's nucleotide sequence