Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt putA1 and repA are separated by 918 nucleotides repA and tap overlap by 8 nucleotides tap and repA2 are separated by 235 nucleotides repA2 and OKFHMN_00410 are separated by 239 nucleotides
OKFHMN_00385: putA1 - CopG family transcriptional regulator, at 83,685 to 83,963
putA1
OKFHMN_00390: repA - incFII family plasmid replication initiator RepA, at 84,882 to 85,739
repA
OKFHMN_00395: tap - RepA leader peptide Tap, at 85,732 to 85,806
tap
OKFHMN_00405: repA2 - Replication regulatory protein repA2, at 86,042 to 86,296
repA2
OKFHMN_00410: OKFHMN_00410 - W0003, at 86,536 to 86,874
_00410
Position (kb) 84
85
86 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 83.906 kb on + strand, within putA1 at 84.031 kb on + strand at 84.050 kb on + strand at 84.051 kb on - strand at 84.051 kb on - strand at 84.051 kb on - strand at 84.051 kb on - strand at 84.051 kb on - strand at 84.060 kb on + strand at 84.060 kb on + strand at 84.061 kb on - strand at 84.069 kb on + strand at 84.070 kb on - strand at 84.070 kb on - strand at 84.075 kb on + strand at 84.075 kb on + strand at 84.075 kb on + strand at 84.076 kb on - strand at 84.076 kb on - strand at 84.076 kb on - strand at 84.190 kb on + strand at 84.190 kb on + strand at 84.191 kb on - strand at 84.191 kb on - strand at 84.241 kb on + strand at 84.242 kb on - strand at 84.399 kb on - strand at 84.520 kb on + strand at 84.520 kb on + strand at 84.521 kb on - strand at 84.521 kb on - strand at 84.521 kb on - strand at 84.521 kb on - strand at 84.521 kb on - strand at 84.521 kb on - strand at 84.573 kb on + strand at 84.578 kb on - strand at 84.578 kb on - strand at 84.582 kb on - strand at 84.587 kb on - strand at 84.597 kb on + strand at 84.597 kb on + strand at 84.597 kb on + strand at 84.598 kb on - strand at 84.609 kb on + strand at 84.616 kb on + strand at 84.774 kb on - strand at 84.911 kb on - strand at 85.312 kb on + strand, within repA at 85.313 kb on - strand, within repA at 85.313 kb on - strand, within repA at 85.753 kb on + strand, within tap at 85.753 kb on + strand, within tap at 85.754 kb on - strand, within tap at 85.809 kb on - strand at 85.809 kb on - strand at 85.813 kb on - strand at 85.813 kb on - strand at 85.925 kb on + strand at 85.925 kb on + strand at 86.002 kb on - strand at 86.140 kb on + strand, within repA2 at 86.141 kb on - strand, within repA2 at 86.141 kb on - strand, within repA2 at 86.141 kb on - strand, within repA2 at 86.141 kb on - strand, within repA2 at 86.142 kb on + strand, within repA2 at 86.142 kb on + strand, within repA2 at 86.143 kb on - strand, within repA2 at 86.143 kb on - strand, within repA2 at 86.143 kb on - strand, within repA2 at 86.143 kb on - strand, within repA2 at 86.160 kb on - strand, within repA2 at 86.160 kb on - strand, within repA2 at 86.188 kb on + strand, within repA2 at 86.201 kb on + strand, within repA2 at 86.244 kb on + strand, within repA2 at 86.245 kb on - strand, within repA2 at 86.311 kb on - strand at 86.314 kb on + strand at 86.314 kb on + strand at 86.315 kb on - strand at 86.343 kb on - strand at 86.414 kb on - strand at 86.423 kb on + strand at 86.434 kb on - strand at 86.445 kb on + strand at 86.445 kb on + strand at 86.445 kb on + strand at 86.446 kb on - strand at 86.446 kb on - strand at 86.541 kb on + strand at 86.542 kb on - strand at 86.640 kb on + strand, within OKFHMN_00410 at 86.641 kb on - strand, within OKFHMN_00410 at 86.699 kb on - strand, within OKFHMN_00410 at 86.715 kb on - strand, within OKFHMN_00410
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 83,906 + putA1 OKFHMN_00385 0.79 +2.0 84,031 + -0.8 84,050 + -0.2 84,051 - +0.2 84,051 - +1.0 84,051 - -2.3 84,051 - -0.3 84,051 - +0.7 84,060 + +0.3 84,060 + -0.4 84,061 - +0.9 84,069 + +0.6 84,070 - +0.4 84,070 - -2.7 84,075 + -1.9 84,075 + +0.4 84,075 + -0.7 84,076 - +1.9 84,076 - -2.5 84,076 - -0.8 84,190 + -0.9 84,190 + -2.1 84,191 - -3.5 84,191 - +0.4 84,241 + -4.0 84,242 - -0.1 84,399 - -0.6 84,520 + -4.0 84,520 + -1.6 84,521 - -1.6 84,521 - -0.7 84,521 - -1.5 84,521 - -1.2 84,521 - -2.5 84,521 - -0.3 84,573 + -0.0 84,578 - -0.5 84,578 - -0.1 84,582 - -1.4 84,587 - -1.6 84,597 + -1.8 84,597 + -0.6 84,597 + -1.1 84,598 - +2.6 84,609 + -0.6 84,616 + -0.0 84,774 - -0.1 84,911 - +1.9 85,312 + repA OKFHMN_00390 0.50 -0.4 85,313 - repA OKFHMN_00390 0.50 -0.5 85,313 - repA OKFHMN_00390 0.50 -0.6 85,753 + tap OKFHMN_00395 0.28 +0.2 85,753 + tap OKFHMN_00395 0.28 +2.5 85,754 - tap OKFHMN_00395 0.29 +1.8 85,809 - -2.8 85,809 - +1.2 85,813 - +1.9 85,813 - +0.6 85,925 + +1.8 85,925 + -0.2 86,002 - -1.2 86,140 + repA2 OKFHMN_00405 0.38 -0.8 86,141 - repA2 OKFHMN_00405 0.39 -0.9 86,141 - repA2 OKFHMN_00405 0.39 -4.5 86,141 - repA2 OKFHMN_00405 0.39 -3.1 86,141 - repA2 OKFHMN_00405 0.39 -6.1 86,142 + repA2 OKFHMN_00405 0.39 -0.2 86,142 + repA2 OKFHMN_00405 0.39 -0.6 86,143 - repA2 OKFHMN_00405 0.40 -1.6 86,143 - repA2 OKFHMN_00405 0.40 -3.0 86,143 - repA2 OKFHMN_00405 0.40 -1.4 86,143 - repA2 OKFHMN_00405 0.40 -2.1 86,160 - repA2 OKFHMN_00405 0.46 -1.4 86,160 - repA2 OKFHMN_00405 0.46 -2.2 86,188 + repA2 OKFHMN_00405 0.57 -0.9 86,201 + repA2 OKFHMN_00405 0.62 -1.1 86,244 + repA2 OKFHMN_00405 0.79 +0.2 86,245 - repA2 OKFHMN_00405 0.80 -0.8 86,311 - -1.7 86,314 + +2.1 86,314 + -2.7 86,315 - -2.7 86,343 - -0.8 86,414 - -0.1 86,423 + -0.1 86,434 - -2.1 86,445 + -0.3 86,445 + +1.1 86,445 + -0.6 86,446 - -0.2 86,446 - -1.4 86,541 + -0.1 86,542 - +0.4 86,640 + OKFHMN_00410 0.31 -0.0 86,641 - OKFHMN_00410 0.31 +0.4 86,699 - OKFHMN_00410 0.48 -0.4 86,715 - OKFHMN_00410 0.53 +1.7
Or see this region's nucleotide sequence