Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28635

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoppA and oppB are separated by 85 nucleotidesoppB and oppC are separated by 14 nucleotidesoppC and oppD are separated by 11 nucleotides OKFHMN_28630: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 5,492,598 to 5,494,274 oppA OKFHMN_28635: oppB - oligopeptide ABC transporter permease OppB, at 5,494,360 to 5,495,280 oppB OKFHMN_28640: oppC - oligopeptide ABC transporter permease OppC, at 5,495,295 to 5,496,203 oppC OKFHMN_28645: oppD - murein tripeptide/oligopeptide ABC transporter ATP-binding protein OppD, at 5,496,215 to 5,497,228 oppD Position (kb) 5494 5495 5496Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5493.813 kb on + strand, within oppAat 5494.171 kb on - strandat 5494.253 kb on + strandat 5494.257 kb on + strandat 5494.257 kb on + strandat 5494.547 kb on - strand, within oppBat 5494.744 kb on + strand, within oppBat 5494.805 kb on + strand, within oppBat 5494.947 kb on + strand, within oppBat 5495.180 kb on + strand, within oppBat 5495.214 kb on - strandat 5495.302 kb on + strandat 5495.978 kb on - strand, within oppC

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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5,493,813 + oppA OKFHMN_28630 0.72 +2.8
5,494,171 - +0.4
5,494,253 + -0.7
5,494,257 + +1.2
5,494,257 + -1.2
5,494,547 - oppB OKFHMN_28635 0.20 +0.3
5,494,744 + oppB OKFHMN_28635 0.42 +0.2
5,494,805 + oppB OKFHMN_28635 0.48 +0.3
5,494,947 + oppB OKFHMN_28635 0.64 -2.2
5,495,180 + oppB OKFHMN_28635 0.89 -2.4
5,495,214 - +1.4
5,495,302 + -0.4
5,495,978 - oppC OKFHMN_28640 0.75 +2.4

Or see this region's nucleotide sequence