Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfB and ynfA are separated by 134 nucleotides ynfA and OKFHMN_27805 are separated by 205 nucleotides OKFHMN_27805 and OKFHMN_27810 are separated by 500 nucleotides OKFHMN_27810 and OKFHMN_27815 are separated by 34 nucleotides
OKFHMN_27795: ynfB - DUF1283 domain-containing protein, at 5,354,469 to 5,354,810
ynfB
OKFHMN_27800: ynfA - YnfA family protein, at 5,354,945 to 5,355,271
ynfA
OKFHMN_27805: OKFHMN_27805 - Starvation-sensing protein, at 5,355,477 to 5,355,569
_27805
OKFHMN_27810: OKFHMN_27810 - Integrase, cryptic prophage CP-933P, at 5,356,070 to 5,356,225
_27810
OKFHMN_27815: OKFHMN_27815 - DNA-binding protein, at 5,356,260 to 5,356,496
_27815
Position (kb) 5355
5356 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5354.562 kb on - strand, within ynfB at 5354.825 kb on - strand at 5354.835 kb on - strand at 5354.889 kb on - strand at 5354.900 kb on - strand at 5354.902 kb on + strand at 5354.914 kb on + strand at 5354.975 kb on - strand at 5355.091 kb on - strand, within ynfA at 5355.143 kb on + strand, within ynfA at 5355.985 kb on - strand at 5356.181 kb on - strand, within OKFHMN_27810 at 5356.359 kb on - strand, within OKFHMN_27815 at 5356.380 kb on - strand, within OKFHMN_27815 at 5356.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 5,354,562 - ynfB OKFHMN_27795 0.27 -1.6 5,354,825 - -1.9 5,354,835 - +0.7 5,354,889 - +0.7 5,354,900 - +1.7 5,354,902 + +1.1 5,354,914 + +0.9 5,354,975 - -0.5 5,355,091 - ynfA OKFHMN_27800 0.45 +2.8 5,355,143 + ynfA OKFHMN_27800 0.61 +1.6 5,355,985 - +1.1 5,356,181 - OKFHMN_27810 0.71 +0.1 5,356,359 - OKFHMN_27815 0.42 +0.7 5,356,380 - OKFHMN_27815 0.51 +0.5 5,356,555 + +0.5
Or see this region's nucleotide sequence