Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27190

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiO and ydiF are separated by 13 nucleotidesydiF and aroD are separated by 144 nucleotidesaroD and ydiB are separated by 30 nucleotides OKFHMN_27185: ydiO - acyl-CoA dehydrogenase, at 5,232,359 to 5,233,510 ydiO OKFHMN_27190: ydiF - Acetate CoA-transferase YdiF, at 5,233,524 to 5,235,119 ydiF OKFHMN_27195: aroD - 3-dehydroquinate dehydratase, at 5,235,264 to 5,236,022 aroD OKFHMN_27200: ydiB - quinate/shikimate dehydrogenase, at 5,236,053 to 5,236,919 ydiB Position (kb) 5233 5234 5235 5236Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 5232.931 kb on + strand, within ydiOat 5233.019 kb on - strand, within ydiOat 5233.399 kb on - strandat 5233.723 kb on + strand, within ydiFat 5234.343 kb on + strand, within ydiFat 5234.375 kb on + strand, within ydiFat 5234.506 kb on + strand, within ydiFat 5234.960 kb on - strandat 5235.585 kb on - strand, within aroD

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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5,232,931 + ydiO OKFHMN_27185 0.50 +0.3
5,233,019 - ydiO OKFHMN_27185 0.57 +0.2
5,233,399 - -2.9
5,233,723 + ydiF OKFHMN_27190 0.12 +0.3
5,234,343 + ydiF OKFHMN_27190 0.51 -0.6
5,234,375 + ydiF OKFHMN_27190 0.53 +0.4
5,234,506 + ydiF OKFHMN_27190 0.62 -0.0
5,234,960 - +1.4
5,235,585 - aroD OKFHMN_27195 0.42 -6.6

Or see this region's nucleotide sequence