Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ada and alkB overlap by 1 nucleotides alkB and yojI are separated by 75 nucleotides yojI and mqo are separated by 217 nucleotides
OKFHMN_23210: ada - bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada, at 4,529,965 to 4,531,029
ada
OKFHMN_23215: alkB - DNA oxidative demethylase AlkB, at 4,531,029 to 4,531,679
alkB
OKFHMN_23220: yojI - microcin J25 efflux ABC transporter YojI, at 4,531,755 to 4,533,398
yojI
OKFHMN_23225: mqo - malate dehydrogenase (quinone), at 4,533,616 to 4,535,262
mqo
Position (kb) 4531
4532
4533
4534 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4530.917 kb on + strand, within ada at 4531.027 kb on + strand at 4531.027 kb on + strand at 4531.028 kb on - strand at 4531.028 kb on - strand at 4531.139 kb on + strand, within alkB at 4531.139 kb on + strand, within alkB at 4531.193 kb on + strand, within alkB at 4531.193 kb on + strand, within alkB at 4531.193 kb on + strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.194 kb on - strand, within alkB at 4531.340 kb on + strand, within alkB at 4531.428 kb on - strand, within alkB at 4531.584 kb on + strand, within alkB at 4531.731 kb on + strand at 4531.799 kb on + strand at 4532.006 kb on + strand, within yojI at 4532.015 kb on + strand, within yojI at 4532.176 kb on - strand, within yojI at 4532.394 kb on + strand, within yojI at 4532.415 kb on + strand, within yojI at 4532.426 kb on + strand, within yojI at 4532.427 kb on - strand, within yojI at 4532.937 kb on + strand, within yojI at 4532.963 kb on + strand, within yojI at 4533.073 kb on + strand, within yojI at 4533.086 kb on + strand, within yojI at 4533.288 kb on + strand at 4533.288 kb on + strand at 4533.441 kb on - strand at 4533.441 kb on - strand at 4533.467 kb on - strand at 4533.601 kb on + strand at 4533.716 kb on + strand at 4533.716 kb on + strand at 4533.761 kb on - strand at 4533.764 kb on + strand at 4533.886 kb on + strand, within mqo at 4533.886 kb on + strand, within mqo at 4534.126 kb on + strand, within mqo at 4534.166 kb on + strand, within mqo at 4534.237 kb on + strand, within mqo
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 4,530,917 + ada OKFHMN_23210 0.89 -0.6 4,531,027 + -0.4 4,531,027 + -1.6 4,531,028 - +0.7 4,531,028 - -1.2 4,531,139 + alkB OKFHMN_23215 0.17 +0.2 4,531,139 + alkB OKFHMN_23215 0.17 -1.0 4,531,193 + alkB OKFHMN_23215 0.25 +0.6 4,531,193 + alkB OKFHMN_23215 0.25 +0.2 4,531,193 + alkB OKFHMN_23215 0.25 -1.8 4,531,194 - alkB OKFHMN_23215 0.25 +0.4 4,531,194 - alkB OKFHMN_23215 0.25 +1.0 4,531,194 - alkB OKFHMN_23215 0.25 +1.3 4,531,194 - alkB OKFHMN_23215 0.25 -0.5 4,531,194 - alkB OKFHMN_23215 0.25 +0.2 4,531,194 - alkB OKFHMN_23215 0.25 +0.4 4,531,340 + alkB OKFHMN_23215 0.48 -0.4 4,531,428 - alkB OKFHMN_23215 0.61 -1.2 4,531,584 + alkB OKFHMN_23215 0.85 +2.0 4,531,731 + +0.4 4,531,799 + +0.2 4,532,006 + yojI OKFHMN_23220 0.15 -0.1 4,532,015 + yojI OKFHMN_23220 0.16 -0.6 4,532,176 - yojI OKFHMN_23220 0.26 +1.0 4,532,394 + yojI OKFHMN_23220 0.39 -1.0 4,532,415 + yojI OKFHMN_23220 0.40 +0.5 4,532,426 + yojI OKFHMN_23220 0.41 -0.4 4,532,427 - yojI OKFHMN_23220 0.41 +0.4 4,532,937 + yojI OKFHMN_23220 0.72 +0.4 4,532,963 + yojI OKFHMN_23220 0.73 +0.1 4,533,073 + yojI OKFHMN_23220 0.80 +0.7 4,533,086 + yojI OKFHMN_23220 0.81 -0.6 4,533,288 + -0.8 4,533,288 + +0.6 4,533,441 - +0.4 4,533,441 - -0.7 4,533,467 - -0.6 4,533,601 + +0.9 4,533,716 + +0.6 4,533,716 + -0.4 4,533,761 - +0.8 4,533,764 + +0.5 4,533,886 + mqo OKFHMN_23225 0.16 +1.5 4,533,886 + mqo OKFHMN_23225 0.16 -1.1 4,534,126 + mqo OKFHMN_23225 0.31 +1.1 4,534,166 + mqo OKFHMN_23225 0.33 -0.4 4,534,237 + mqo OKFHMN_23225 0.38 -2.0
Or see this region's nucleotide sequence