Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20275

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrG and eno are separated by 87 nucleotideseno and ygcG are separated by 59 nucleotidesygcG and ygcG overlap by 4 nucleotides OKFHMN_20270: pyrG - CTP synthase (glutamine hydrolyzing), at 3,944,325 to 3,945,962 pyrG OKFHMN_20275: eno - phosphopyruvate hydratase, at 3,946,050 to 3,947,348 eno OKFHMN_20280: ygcG - TPM-phosphatase domain-containing protein, at 3,947,408 to 3,948,322 ygcG OKFHMN_20285: ygcG - TPM-phosphatase domain-containing protein, at 3,948,319 to 3,949,467 ygcG Position (kb) 3946 3947 3948Strain fitness (log2 ratio) -1 0 1at 3945.960 kb on + strandat 3945.960 kb on + strandat 3947.346 kb on + strandat 3947.358 kb on + strandat 3947.358 kb on + strandat 3947.534 kb on - strand, within ygcGat 3947.603 kb on + strand, within ygcGat 3947.751 kb on + strand, within ygcGat 3947.815 kb on - strand, within ygcGat 3947.817 kb on + strand, within ygcGat 3947.930 kb on - strand, within ygcGat 3948.085 kb on + strand, within ygcGat 3948.132 kb on - strand, within ygcGat 3948.155 kb on - strand, within ygcGat 3948.211 kb on + strand, within ygcGat 3948.218 kb on - strand, within ygcG

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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3,945,960 + -0.1
3,945,960 + -0.3
3,947,346 + -0.5
3,947,358 + -0.5
3,947,358 + +1.4
3,947,534 - ygcG OKFHMN_20280 0.14 +0.6
3,947,603 + ygcG OKFHMN_20280 0.21 +1.5
3,947,751 + ygcG OKFHMN_20280 0.37 +0.0
3,947,815 - ygcG OKFHMN_20280 0.44 -0.6
3,947,817 + ygcG OKFHMN_20280 0.45 -0.5
3,947,930 - ygcG OKFHMN_20280 0.57 -1.0
3,948,085 + ygcG OKFHMN_20280 0.74 +0.6
3,948,132 - ygcG OKFHMN_20280 0.79 +1.0
3,948,155 - ygcG OKFHMN_20280 0.82 +0.9
3,948,211 + ygcG OKFHMN_20280 0.88 +1.7
3,948,218 - ygcG OKFHMN_20280 0.89 +0.5

Or see this region's nucleotide sequence