Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15305

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescS and escT overlap by 1 nucleotidesescT and escU overlap by 8 nucleotidesescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotides OKFHMN_15295: escS - type III secretion system LEE export apparatus protein EscS, at 2,957,747 to 2,958,016 escS OKFHMN_15300: escT - type III secretion system LEE export apparatus protein EscT, at 2,958,016 to 2,958,792 escT OKFHMN_15305: escU - type III secretion system LEE export apparatus switch protein EscU, at 2,958,785 to 2,959,822 escU OKFHMN_15310: etgA - type III secretion system LEE muramidase EtgA, at 2,959,819 to 2,960,277 etgA OKFHMN_15315: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 2,960,473 to 2,960,844 grlR Position (kb) 2958 2959 2960Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2957.846 kb on - strand, within escSat 2957.846 kb on - strand, within escSat 2957.985 kb on + strand, within escSat 2958.026 kb on + strandat 2958.293 kb on + strand, within escTat 2958.339 kb on - strand, within escTat 2958.363 kb on - strand, within escTat 2958.449 kb on + strand, within escTat 2958.660 kb on - strand, within escTat 2959.596 kb on - strand, within escUat 2959.599 kb on - strand, within escUat 2959.744 kb on + strandat 2959.806 kb on + strandat 2959.857 kb on - strandat 2960.193 kb on + strand, within etgAat 2960.232 kb on - strandat 2960.422 kb on - strandat 2960.539 kb on - strand, within grlRat 2960.624 kb on + strand, within grlRat 2960.690 kb on + strand, within grlRat 2960.806 kb on - strand, within grlR

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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2,957,846 - escS OKFHMN_15295 0.37 -0.3
2,957,846 - escS OKFHMN_15295 0.37 +0.5
2,957,985 + escS OKFHMN_15295 0.88 -0.3
2,958,026 + -3.1
2,958,293 + escT OKFHMN_15300 0.36 -0.7
2,958,339 - escT OKFHMN_15300 0.42 -1.8
2,958,363 - escT OKFHMN_15300 0.45 -0.2
2,958,449 + escT OKFHMN_15300 0.56 -1.0
2,958,660 - escT OKFHMN_15300 0.83 +0.3
2,959,596 - escU OKFHMN_15305 0.78 -0.1
2,959,599 - escU OKFHMN_15305 0.78 +0.6
2,959,744 + +0.6
2,959,806 + -4.2
2,959,857 - +0.8
2,960,193 + etgA OKFHMN_15310 0.81 -0.5
2,960,232 - +1.9
2,960,422 - +2.3
2,960,539 - grlR OKFHMN_15315 0.18 -0.2
2,960,624 + grlR OKFHMN_15315 0.41 -0.9
2,960,690 + grlR OKFHMN_15315 0.58 -0.5
2,960,806 - grlR OKFHMN_15315 0.90 +0.8

Or see this region's nucleotide sequence