Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escE and cesAB are separated by 12 nucleotides cesAB and escK overlap by 4 nucleotides escK and escL overlap by 14 nucleotides escL and escR are separated by 4 nucleotides escR and escS overlap by 1 nucleotides escS and escT overlap by 1 nucleotides
OKFHMN_15270: escE - chaperone for EscF, at 2,955,308 to 2,955,526
escE
OKFHMN_15275: cesAB - chaperone for EspA and EspB, at 2,955,539 to 2,955,853
cesAB
OKFHMN_15280: escK - type III secretion system sorting platform component, at 2,955,850 to 2,956,449
escK
OKFHMN_15285: escL - negative regulator EscL, stator protein, at 2,956,436 to 2,957,089
escL
OKFHMN_15290: escR - Type III secretion systemminor export apparatus protein EscR, at 2,957,094 to 2,957,747
escR
OKFHMN_15295: escS - type III secretion system LEE export apparatus protein EscS, at 2,957,747 to 2,958,016
escS
OKFHMN_15300: escT - type III secretion system LEE export apparatus protein EscT, at 2,958,016 to 2,958,792
escT
Position (kb)
2956
2957
2958 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2955.757 kb on + strand, within cesAB at 2955.838 kb on + strand at 2955.971 kb on + strand, within escK at 2956.867 kb on + strand, within escL at 2956.954 kb on + strand, within escL at 2957.846 kb on - strand, within escS at 2957.846 kb on - strand, within escS at 2957.985 kb on + strand, within escS at 2958.026 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 2,955,757 + cesAB OKFHMN_15275 0.69 +1.9 2,955,838 + +0.7 2,955,971 + escK OKFHMN_15280 0.20 -2.1 2,956,867 + escL OKFHMN_15285 0.66 -0.4 2,956,954 + escL OKFHMN_15285 0.79 +0.1 2,957,846 - escS OKFHMN_15295 0.37 -0.3 2,957,846 - escS OKFHMN_15295 0.37 +0.5 2,957,985 + escS OKFHMN_15295 0.88 -0.3 2,958,026 + -3.1
Or see this region's nucleotide sequence