Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10105

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntarfB and yaeQ overlap by 4 nucleotidesyaeQ and yaeP are separated by 165 nucleotidesyaeP and rof overlap by 14 nucleotidesrof and tilS are separated by 52 nucleotides OKFHMN_10095: arfB - aminoacyl-tRNA hydrolase, at 1,884,605 to 1,885,015 arfB OKFHMN_10100: yaeQ - Uncharacterized protein YaeQ, at 1,885,012 to 1,885,557 yaeQ OKFHMN_10105: yaeP - YaeP family protein, at 1,885,723 to 1,885,923 yaeP OKFHMN_10110: rof - Rho-binding antiterminator, at 1,885,910 to 1,886,170 rof OKFHMN_10115: tilS - tRNA lysidine(34) synthetase TilS, at 1,886,223 to 1,887,518 tilS Position (kb) 1885 1886Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1884.809 kb on + strand, within arfBat 1884.862 kb on - strand, within arfBat 1884.864 kb on + strand, within arfBat 1884.865 kb on - strand, within arfBat 1884.923 kb on - strand, within arfBat 1885.054 kb on - strandat 1885.059 kb on + strandat 1885.114 kb on - strand, within yaeQat 1885.233 kb on + strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.234 kb on - strand, within yaeQat 1885.236 kb on + strand, within yaeQat 1885.236 kb on + strand, within yaeQat 1885.237 kb on - strand, within yaeQat 1885.238 kb on + strand, within yaeQat 1885.238 kb on + strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.239 kb on - strand, within yaeQat 1885.242 kb on + strand, within yaeQat 1885.440 kb on + strand, within yaeQat 1885.599 kb on - strandat 1885.640 kb on - strandat 1885.641 kb on + strandat 1885.664 kb on + strandat 1885.664 kb on + strandat 1885.742 kb on + strandat 1885.757 kb on - strand, within yaePat 1885.781 kb on + strand, within yaePat 1885.782 kb on - strand, within yaePat 1885.787 kb on - strand, within yaePat 1885.924 kb on + strandat 1885.924 kb on + strandat 1885.925 kb on - strandat 1885.928 kb on + strandat 1885.928 kb on + strandat 1885.928 kb on + strandat 1885.929 kb on - strandat 1885.986 kb on + strand, within rofat 1886.066 kb on + strand, within rofat 1886.103 kb on + strand, within rofat 1886.103 kb on + strand, within rofat 1886.104 kb on - strand, within rofat 1886.120 kb on - strand, within rofat 1886.195 kb on + strandat 1886.206 kb on - strandat 1886.224 kb on + strandat 1886.225 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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1,884,809 + arfB OKFHMN_10095 0.50 -3.1
1,884,862 - arfB OKFHMN_10095 0.63 +0.3
1,884,864 + arfB OKFHMN_10095 0.63 +0.8
1,884,865 - arfB OKFHMN_10095 0.63 -1.6
1,884,923 - arfB OKFHMN_10095 0.77 -0.2
1,885,054 - -0.2
1,885,059 + +1.5
1,885,114 - yaeQ OKFHMN_10100 0.19 +0.0
1,885,233 + yaeQ OKFHMN_10100 0.40 +1.6
1,885,234 - yaeQ OKFHMN_10100 0.41 -1.2
1,885,234 - yaeQ OKFHMN_10100 0.41 +0.6
1,885,234 - yaeQ OKFHMN_10100 0.41 -0.1
1,885,236 + yaeQ OKFHMN_10100 0.41 +0.3
1,885,236 + yaeQ OKFHMN_10100 0.41 -0.2
1,885,237 - yaeQ OKFHMN_10100 0.41 -0.1
1,885,238 + yaeQ OKFHMN_10100 0.41 +0.4
1,885,238 + yaeQ OKFHMN_10100 0.41 -0.5
1,885,239 - yaeQ OKFHMN_10100 0.42 +1.1
1,885,239 - yaeQ OKFHMN_10100 0.42 -0.1
1,885,239 - yaeQ OKFHMN_10100 0.42 +0.4
1,885,242 + yaeQ OKFHMN_10100 0.42 +0.6
1,885,440 + yaeQ OKFHMN_10100 0.78 +0.3
1,885,599 - -3.0
1,885,640 - +0.8
1,885,641 + +2.5
1,885,664 + +0.3
1,885,664 + +0.8
1,885,742 + +0.7
1,885,757 - yaeP OKFHMN_10105 0.17 -0.2
1,885,781 + yaeP OKFHMN_10105 0.29 -0.1
1,885,782 - yaeP OKFHMN_10105 0.29 +1.3
1,885,787 - yaeP OKFHMN_10105 0.32 +0.6
1,885,924 + +1.0
1,885,924 + +0.7
1,885,925 - +1.1
1,885,928 + +0.5
1,885,928 + +0.8
1,885,928 + +1.0
1,885,929 - -0.2
1,885,986 + rof OKFHMN_10110 0.29 +0.5
1,886,066 + rof OKFHMN_10110 0.60 -1.2
1,886,103 + rof OKFHMN_10110 0.74 +0.1
1,886,103 + rof OKFHMN_10110 0.74 -0.0
1,886,104 - rof OKFHMN_10110 0.74 -0.1
1,886,120 - rof OKFHMN_10110 0.80 +0.7
1,886,195 + +0.2
1,886,206 - -1.6
1,886,224 + -1.6
1,886,225 - +0.2

Or see this region's nucleotide sequence