Experiment: L-Arabinose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolQ and ybgC overlap by 4 nucleotides ybgC and ybgE are separated by 149 nucleotides ybgE and cydX overlap by 1 nucleotides cydX and cydB are separated by 14 nucleotides cydB and cydA are separated by 15 nucleotides
OKFHMN_06820: tolQ - Tol-Pal system protein TolQ, at 1,212,953 to 1,213,645
tolQ
OKFHMN_06825: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 1,213,642 to 1,214,046
ybgC
OKFHMN_06830: ybgE - cyd operon protein YbgE, at 1,214,196 to 1,214,489
ybgE
OKFHMN_06835: cydX - cytochrome bd-I oxidase subunit CydX, at 1,214,489 to 1,214,602
cydX
OKFHMN_06840: cydB - cytochrome d ubiquinol oxidase subunit II, at 1,214,617 to 1,215,756
cydB
OKFHMN_06845: cydA - cytochrome ubiquinol oxidase subunit I, at 1,215,772 to 1,217,340
cydA
Position (kb)
1214
1215
1216 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1213.743 kb on + strand, within ybgC at 1213.761 kb on + strand, within ybgC at 1213.762 kb on - strand, within ybgC at 1213.824 kb on + strand, within ybgC at 1213.848 kb on - strand, within ybgC at 1213.964 kb on + strand, within ybgC at 1213.965 kb on - strand, within ybgC at 1213.977 kb on - strand, within ybgC at 1214.006 kb on + strand at 1214.007 kb on - strand at 1214.007 kb on - strand at 1214.007 kb on - strand at 1214.009 kb on + strand at 1214.010 kb on - strand at 1214.010 kb on - strand at 1214.041 kb on - strand at 1214.041 kb on - strand at 1214.050 kb on - strand at 1214.084 kb on - strand at 1214.094 kb on - strand at 1214.134 kb on + strand at 1214.396 kb on + strand, within ybgE at 1214.397 kb on - strand, within ybgE at 1214.397 kb on - strand, within ybgE at 1214.483 kb on + strand at 1214.615 kb on - strand at 1214.615 kb on - strand at 1214.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arabinose remove 1,213,743 + ybgC OKFHMN_06825 0.25 -2.2 1,213,761 + ybgC OKFHMN_06825 0.29 -1.3 1,213,762 - ybgC OKFHMN_06825 0.30 -0.9 1,213,824 + ybgC OKFHMN_06825 0.45 -1.5 1,213,848 - ybgC OKFHMN_06825 0.51 -0.5 1,213,964 + ybgC OKFHMN_06825 0.80 -0.3 1,213,965 - ybgC OKFHMN_06825 0.80 +0.1 1,213,977 - ybgC OKFHMN_06825 0.83 +2.4 1,214,006 + +0.7 1,214,007 - +0.2 1,214,007 - +0.3 1,214,007 - +0.5 1,214,009 + +0.3 1,214,010 - +0.1 1,214,010 - +0.1 1,214,041 - -0.9 1,214,041 - +0.8 1,214,050 - +0.5 1,214,084 - +0.2 1,214,094 - -2.5 1,214,134 + +1.9 1,214,396 + ybgE OKFHMN_06830 0.68 +0.6 1,214,397 - ybgE OKFHMN_06830 0.68 +0.1 1,214,397 - ybgE OKFHMN_06830 0.68 -2.4 1,214,483 + -2.7 1,214,615 - -1.6 1,214,615 - -1.6 1,214,619 - +1.1
Or see this region's nucleotide sequence