Strain Fitness in Escherichia coli ECRC62 around BNILDI_23120

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmhpR and lacI are separated by 67 nucleotideslacI and lacZ are separated by 122 nucleotides BNILDI_23115: mhpR - DNA-binding transcriptional regulator, at 4,653,364 to 4,654,311 mhpR BNILDI_23120: lacI - DNA-binding transcriptional repressor LacI, at 4,654,379 to 4,655,470 lacI BNILDI_23125: lacZ - beta-galactosidase, at 4,655,593 to 4,658,667 lacZ Position (kb) 4654 4655 4656Strain fitness (log2 ratio) -2 -1 0 1at 4653.387 kb on + strandat 4653.533 kb on + strand, within mhpRat 4653.655 kb on + strand, within mhpRat 4653.713 kb on + strand, within mhpRat 4654.003 kb on + strand, within mhpRat 4654.004 kb on - strand, within mhpRat 4654.114 kb on - strand, within mhpRat 4654.168 kb on + strand, within mhpRat 4654.168 kb on + strand, within mhpRat 4654.169 kb on - strand, within mhpRat 4654.169 kb on - strand, within mhpRat 4654.169 kb on - strand, within mhpRat 4654.170 kb on + strand, within mhpRat 4654.407 kb on - strandat 4654.436 kb on + strandat 4654.527 kb on - strand, within lacIat 4654.527 kb on - strand, within lacIat 4654.636 kb on + strand, within lacIat 4654.684 kb on - strand, within lacIat 4654.709 kb on - strand, within lacIat 4654.760 kb on - strand, within lacIat 4654.864 kb on + strand, within lacIat 4654.865 kb on - strand, within lacIat 4654.865 kb on - strand, within lacIat 4654.993 kb on + strand, within lacIat 4654.997 kb on + strand, within lacIat 4654.997 kb on + strand, within lacIat 4655.233 kb on + strand, within lacIat 4655.425 kb on - strandat 4655.484 kb on - strandat 4655.493 kb on + strandat 4655.521 kb on + strandat 4655.628 kb on - strandat 4655.628 kb on - strandat 4655.668 kb on - strandat 4655.893 kb on + strandat 4656.181 kb on + strand, within lacZat 4656.181 kb on + strand, within lacZat 4656.198 kb on + strand, within lacZat 4656.199 kb on - strand, within lacZat 4656.289 kb on - strand, within lacZat 4656.311 kb on + strand, within lacZat 4656.444 kb on + strand, within lacZat 4656.445 kb on - strand, within lacZat 4656.448 kb on + strand, within lacZat 4656.448 kb on + strand, within lacZat 4656.448 kb on + strand, within lacZat 4656.449 kb on - strand, within lacZat 4656.467 kb on + strand, within lacZ

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,653,387 + +0.7
4,653,533 + mhpR BNILDI_23115 0.18 +0.1
4,653,655 + mhpR BNILDI_23115 0.31 +0.3
4,653,713 + mhpR BNILDI_23115 0.37 +0.1
4,654,003 + mhpR BNILDI_23115 0.67 +0.5
4,654,004 - mhpR BNILDI_23115 0.68 -1.1
4,654,114 - mhpR BNILDI_23115 0.79 +0.1
4,654,168 + mhpR BNILDI_23115 0.85 -0.2
4,654,168 + mhpR BNILDI_23115 0.85 +0.5
4,654,169 - mhpR BNILDI_23115 0.85 -0.5
4,654,169 - mhpR BNILDI_23115 0.85 -0.9
4,654,169 - mhpR BNILDI_23115 0.85 -0.7
4,654,170 + mhpR BNILDI_23115 0.85 +0.5
4,654,407 - +0.2
4,654,436 + +0.6
4,654,527 - lacI BNILDI_23120 0.14 +1.6
4,654,527 - lacI BNILDI_23120 0.14 +0.6
4,654,636 + lacI BNILDI_23120 0.24 -1.0
4,654,684 - lacI BNILDI_23120 0.28 -1.0
4,654,709 - lacI BNILDI_23120 0.30 -0.5
4,654,760 - lacI BNILDI_23120 0.35 +0.5
4,654,864 + lacI BNILDI_23120 0.44 -0.5
4,654,865 - lacI BNILDI_23120 0.45 -0.4
4,654,865 - lacI BNILDI_23120 0.45 +0.3
4,654,993 + lacI BNILDI_23120 0.56 -0.3
4,654,997 + lacI BNILDI_23120 0.57 -0.9
4,654,997 + lacI BNILDI_23120 0.57 +0.1
4,655,233 + lacI BNILDI_23120 0.78 -1.2
4,655,425 - +0.3
4,655,484 - -0.5
4,655,493 + +0.5
4,655,521 + -1.0
4,655,628 - +0.3
4,655,628 - -0.0
4,655,668 - -0.0
4,655,893 + -2.3
4,656,181 + lacZ BNILDI_23125 0.19 -0.7
4,656,181 + lacZ BNILDI_23125 0.19 -0.2
4,656,198 + lacZ BNILDI_23125 0.20 +0.6
4,656,199 - lacZ BNILDI_23125 0.20 -0.1
4,656,289 - lacZ BNILDI_23125 0.23 -0.5
4,656,311 + lacZ BNILDI_23125 0.23 -1.3
4,656,444 + lacZ BNILDI_23125 0.28 +0.2
4,656,445 - lacZ BNILDI_23125 0.28 +1.2
4,656,448 + lacZ BNILDI_23125 0.28 +1.2
4,656,448 + lacZ BNILDI_23125 0.28 -0.2
4,656,448 + lacZ BNILDI_23125 0.28 +1.7
4,656,449 - lacZ BNILDI_23125 0.28 -0.4
4,656,467 + lacZ BNILDI_23125 0.28 +0.7

Or see this region's nucleotide sequence