Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcsA and yaiO are separated by 560 nucleotides yaiO and frmR are separated by 185 nucleotides frmR and frmA are separated by 34 nucleotides
BNILDI_23050: bcsA - Glycosyl transferase family 2, at 4,639,803 to 4,640,999
bcsA
BNILDI_23055: yaiO - Outer membrane protein YaiO, at 4,641,560 to 4,642,333
yaiO
BNILDI_23060: frmR - formaldehyde-responsive transcriptional repressor FrmR, at 4,642,519 to 4,642,794
frmR
BNILDI_23065: frmA - S-(hydroxymethyl)glutathione dehydrogenase, at 4,642,829 to 4,643,938
frmA
Position (kb)
4641
4642
4643 Strain fitness (log2 ratio)
-1
0
1 at 4640.577 kb on - strand, within bcsA at 4640.589 kb on - strand, within bcsA at 4640.627 kb on - strand, within bcsA at 4640.667 kb on + strand, within bcsA at 4640.836 kb on + strand, within bcsA at 4641.262 kb on - strand at 4641.309 kb on - strand at 4641.313 kb on - strand at 4641.392 kb on + strand at 4641.400 kb on + strand at 4641.400 kb on + strand at 4641.428 kb on + strand at 4641.436 kb on - strand at 4641.807 kb on - strand, within yaiO at 4641.953 kb on + strand, within yaiO at 4642.063 kb on + strand, within yaiO at 4642.064 kb on - strand, within yaiO at 4642.068 kb on - strand, within yaiO at 4642.069 kb on + strand, within yaiO at 4642.069 kb on + strand, within yaiO at 4642.070 kb on - strand, within yaiO at 4642.076 kb on - strand, within yaiO at 4642.118 kb on - strand, within yaiO at 4642.125 kb on + strand, within yaiO at 4642.126 kb on - strand, within yaiO at 4642.126 kb on - strand, within yaiO at 4642.151 kb on + strand, within yaiO at 4642.151 kb on + strand, within yaiO at 4642.152 kb on - strand, within yaiO at 4642.158 kb on - strand, within yaiO at 4642.158 kb on - strand, within yaiO at 4642.238 kb on - strand, within yaiO at 4642.238 kb on - strand, within yaiO at 4642.258 kb on + strand at 4642.259 kb on - strand at 4642.289 kb on + strand at 4642.290 kb on - strand at 4642.445 kb on + strand at 4642.449 kb on - strand at 4642.506 kb on - strand at 4642.631 kb on + strand, within frmR at 4642.925 kb on - strand at 4642.993 kb on - strand, within frmA at 4643.208 kb on - strand, within frmA at 4643.226 kb on - strand, within frmA at 4643.232 kb on - strand, within frmA at 4643.259 kb on - strand, within frmA
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,640,577 - bcsA BNILDI_23050 0.65 +0.1 4,640,589 - bcsA BNILDI_23050 0.66 -0.7 4,640,627 - bcsA BNILDI_23050 0.69 -0.2 4,640,667 + bcsA BNILDI_23050 0.72 +0.8 4,640,836 + bcsA BNILDI_23050 0.86 -0.1 4,641,262 - +0.2 4,641,309 - +1.1 4,641,313 - +1.1 4,641,392 + +0.7 4,641,400 + +0.0 4,641,400 + -0.3 4,641,428 + -0.2 4,641,436 - +0.1 4,641,807 - yaiO BNILDI_23055 0.32 -1.2 4,641,953 + yaiO BNILDI_23055 0.51 +1.1 4,642,063 + yaiO BNILDI_23055 0.65 +0.7 4,642,064 - yaiO BNILDI_23055 0.65 -0.5 4,642,068 - yaiO BNILDI_23055 0.66 +0.1 4,642,069 + yaiO BNILDI_23055 0.66 -0.1 4,642,069 + yaiO BNILDI_23055 0.66 -0.4 4,642,070 - yaiO BNILDI_23055 0.66 -0.0 4,642,076 - yaiO BNILDI_23055 0.67 -0.1 4,642,118 - yaiO BNILDI_23055 0.72 -0.2 4,642,125 + yaiO BNILDI_23055 0.73 +0.3 4,642,126 - yaiO BNILDI_23055 0.73 +0.3 4,642,126 - yaiO BNILDI_23055 0.73 -0.6 4,642,151 + yaiO BNILDI_23055 0.76 +1.6 4,642,151 + yaiO BNILDI_23055 0.76 +0.3 4,642,152 - yaiO BNILDI_23055 0.76 +0.6 4,642,158 - yaiO BNILDI_23055 0.77 -0.6 4,642,158 - yaiO BNILDI_23055 0.77 -0.1 4,642,238 - yaiO BNILDI_23055 0.88 -0.1 4,642,238 - yaiO BNILDI_23055 0.88 -0.3 4,642,258 + +0.1 4,642,259 - +0.8 4,642,289 + -0.1 4,642,290 - +0.1 4,642,445 + -0.3 4,642,449 - +1.0 4,642,506 - -1.1 4,642,631 + frmR BNILDI_23060 0.41 -1.3 4,642,925 - -1.5 4,642,993 - frmA BNILDI_23065 0.15 +0.8 4,643,208 - frmA BNILDI_23065 0.34 -0.7 4,643,226 - frmA BNILDI_23065 0.36 -0.9 4,643,232 - frmA BNILDI_23065 0.36 -1.3 4,643,259 - frmA BNILDI_23065 0.39 -0.2
Or see this region's nucleotide sequence