Strain Fitness in Escherichia coli ECRC62 around BNILDI_21465

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdpD and kdpE overlap by 4 nucleotideskdpE and speFL are separated by 274 nucleotidesspeFL and speF are separated by 310 nucleotides BNILDI_21460: kdpD - two-component system sensor histidine kinase KdpD, at 4,316,542 to 4,319,226 kdpD BNILDI_21465: kdpE - two-component system response regulator KdpE, at 4,319,223 to 4,319,900 kdpE BNILDI_21470: speFL - Leader peptide SpeFL, at 4,320,175 to 4,320,279 speFL BNILDI_21475: speF - ornithine decarboxylase SpeF, at 4,320,590 to 4,322,788 speF Position (kb) 4319 4320Strain fitness (log2 ratio) -1 0 1at 4318.443 kb on + strand, within kdpDat 4318.444 kb on - strand, within kdpDat 4318.444 kb on - strand, within kdpDat 4318.444 kb on - strand, within kdpDat 4318.565 kb on + strand, within kdpDat 4318.698 kb on + strand, within kdpDat 4318.738 kb on - strand, within kdpDat 4318.915 kb on + strand, within kdpDat 4318.916 kb on - strand, within kdpDat 4319.027 kb on + strandat 4319.034 kb on + strandat 4319.114 kb on + strandat 4319.114 kb on + strandat 4319.115 kb on - strandat 4319.127 kb on + strandat 4319.172 kb on + strandat 4319.220 kb on + strandat 4319.308 kb on - strand, within kdpEat 4319.369 kb on + strand, within kdpEat 4319.515 kb on - strand, within kdpEat 4319.515 kb on - strand, within kdpEat 4319.569 kb on + strand, within kdpEat 4319.717 kb on + strand, within kdpEat 4319.793 kb on + strand, within kdpEat 4319.808 kb on - strand, within kdpEat 4320.130 kb on + strandat 4320.135 kb on + strandat 4320.173 kb on - strandat 4320.205 kb on - strand, within speFLat 4320.325 kb on - strandat 4320.363 kb on + strandat 4320.366 kb on - strandat 4320.459 kb on + strandat 4320.490 kb on + strandat 4320.673 kb on + strandat 4320.853 kb on + strand, within speFat 4320.877 kb on - strand, within speF

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,318,443 + kdpD BNILDI_21460 0.71 -1.7
4,318,444 - kdpD BNILDI_21460 0.71 +0.4
4,318,444 - kdpD BNILDI_21460 0.71 +0.1
4,318,444 - kdpD BNILDI_21460 0.71 -0.4
4,318,565 + kdpD BNILDI_21460 0.75 +0.2
4,318,698 + kdpD BNILDI_21460 0.80 +1.5
4,318,738 - kdpD BNILDI_21460 0.82 -0.8
4,318,915 + kdpD BNILDI_21460 0.88 -0.3
4,318,916 - kdpD BNILDI_21460 0.88 +0.3
4,319,027 + -0.1
4,319,034 + +0.6
4,319,114 + +0.1
4,319,114 + +0.2
4,319,115 - +0.0
4,319,127 + -1.7
4,319,172 + -0.6
4,319,220 + +0.1
4,319,308 - kdpE BNILDI_21465 0.13 +0.4
4,319,369 + kdpE BNILDI_21465 0.22 -0.0
4,319,515 - kdpE BNILDI_21465 0.43 +0.3
4,319,515 - kdpE BNILDI_21465 0.43 -1.5
4,319,569 + kdpE BNILDI_21465 0.51 +1.5
4,319,717 + kdpE BNILDI_21465 0.73 +0.2
4,319,793 + kdpE BNILDI_21465 0.84 -0.2
4,319,808 - kdpE BNILDI_21465 0.86 -0.4
4,320,130 + +1.0
4,320,135 + +0.2
4,320,173 - -0.1
4,320,205 - speFL BNILDI_21470 0.29 -0.2
4,320,325 - -0.5
4,320,363 + +0.1
4,320,366 - +0.2
4,320,459 + +0.0
4,320,490 + +0.0
4,320,673 + -0.1
4,320,853 + speF BNILDI_21475 0.12 -0.3
4,320,877 - speF BNILDI_21475 0.13 +0.4

Or see this region's nucleotide sequence