Strain Fitness in Escherichia coli ECRC62 around BNILDI_21245

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotidestolQ and ybgC overlap by 4 nucleotides BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609 tolA BNILDI_21245: tolR - colicin uptake protein TolR, at 4,269,674 to 4,270,102 tolR BNILDI_21250: tolQ - Tol-Pal system protein TolQ, at 4,270,106 to 4,270,798 tolQ BNILDI_21255: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 4,270,795 to 4,271,199 ybgC Position (kb) 4269 4270 4271Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4269.150 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.497 kb on - strandat 4269.562 kb on - strandat 4269.675 kb on + strandat 4269.675 kb on + strandat 4269.693 kb on - strandat 4269.723 kb on - strand, within tolRat 4269.744 kb on - strand, within tolRat 4270.225 kb on - strand, within tolQat 4270.384 kb on - strand, within tolQat 4270.459 kb on + strand, within tolQat 4270.466 kb on - strand, within tolQat 4270.896 kb on + strand, within ybgCat 4270.897 kb on - strand, within ybgCat 4270.915 kb on - strand, within ybgCat 4270.915 kb on - strand, within ybgCat 4270.966 kb on + strand, within ybgCat 4271.004 kb on - strand, within ybgCat 4271.019 kb on + strand, within ybgCat 4271.019 kb on + strand, within ybgCat 4271.020 kb on - strand, within ybgCat 4271.024 kb on - strand, within ybgC

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,269,150 + tolA BNILDI_21240 0.65 -2.9
4,269,419 + tolA BNILDI_21240 0.85 -5.4
4,269,419 + tolA BNILDI_21240 0.85 -3.9
4,269,497 - +0.3
4,269,562 - -4.6
4,269,675 + -0.7
4,269,675 + -1.3
4,269,693 - +0.2
4,269,723 - tolR BNILDI_21245 0.11 -4.8
4,269,744 - tolR BNILDI_21245 0.16 -1.1
4,270,225 - tolQ BNILDI_21250 0.17 -0.9
4,270,384 - tolQ BNILDI_21250 0.40 -3.3
4,270,459 + tolQ BNILDI_21250 0.51 -2.2
4,270,466 - tolQ BNILDI_21250 0.52 -2.9
4,270,896 + ybgC BNILDI_21255 0.25 -1.2
4,270,897 - ybgC BNILDI_21255 0.25 -0.7
4,270,915 - ybgC BNILDI_21255 0.30 -0.5
4,270,915 - ybgC BNILDI_21255 0.30 +0.5
4,270,966 + ybgC BNILDI_21255 0.42 -2.2
4,271,004 - ybgC BNILDI_21255 0.52 +0.7
4,271,019 + ybgC BNILDI_21255 0.55 -0.0
4,271,019 + ybgC BNILDI_21255 0.55 -0.7
4,271,020 - ybgC BNILDI_21255 0.56 -0.1
4,271,024 - ybgC BNILDI_21255 0.57 -0.6

Or see this region's nucleotide sequence