Experiment: D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolB and tolA are separated by 132 nucleotides tolA and tolR are separated by 64 nucleotides tolR and tolQ are separated by 3 nucleotides
BNILDI_21235: tolB - Tol-Pal system protein TolB, at 4,266,886 to 4,268,178
tolB
BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609
tolA
BNILDI_21245: tolR - colicin uptake protein TolR, at 4,269,674 to 4,270,102
tolR
BNILDI_21250: tolQ - Tol-Pal system protein TolQ, at 4,270,106 to 4,270,798
tolQ
Position (kb)
4268
4269
4270 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 4267.368 kb on - strand, within tolB at 4267.471 kb on + strand, within tolB at 4267.674 kb on - strand, within tolB at 4267.680 kb on + strand, within tolB at 4267.751 kb on + strand, within tolB at 4267.795 kb on + strand, within tolB at 4267.796 kb on - strand, within tolB at 4267.845 kb on - strand, within tolB at 4267.883 kb on - strand, within tolB at 4267.883 kb on - strand, within tolB at 4267.883 kb on - strand, within tolB at 4267.967 kb on - strand, within tolB at 4268.164 kb on + strand at 4268.181 kb on + strand at 4268.182 kb on - strand at 4268.182 kb on - strand at 4268.251 kb on + strand at 4268.252 kb on - strand at 4268.313 kb on - strand at 4268.504 kb on + strand, within tolA at 4268.505 kb on - strand, within tolA at 4268.520 kb on - strand, within tolA at 4268.520 kb on - strand, within tolA at 4268.563 kb on - strand, within tolA at 4268.595 kb on + strand, within tolA at 4268.596 kb on - strand, within tolA at 4269.150 kb on + strand, within tolA at 4269.419 kb on + strand, within tolA at 4269.419 kb on + strand, within tolA at 4269.497 kb on - strand at 4269.562 kb on - strand at 4269.675 kb on + strand at 4269.675 kb on + strand at 4269.693 kb on - strand at 4269.723 kb on - strand, within tolR at 4269.744 kb on - strand, within tolR at 4270.225 kb on - strand, within tolQ at 4270.384 kb on - strand, within tolQ at 4270.459 kb on + strand, within tolQ at 4270.466 kb on - strand, within tolQ
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose remove 4,267,368 - tolB BNILDI_21235 0.37 -4.3 4,267,471 + tolB BNILDI_21235 0.45 -3.4 4,267,674 - tolB BNILDI_21235 0.61 -1.7 4,267,680 + tolB BNILDI_21235 0.61 -2.4 4,267,751 + tolB BNILDI_21235 0.67 -1.1 4,267,795 + tolB BNILDI_21235 0.70 -2.1 4,267,796 - tolB BNILDI_21235 0.70 -0.2 4,267,845 - tolB BNILDI_21235 0.74 +0.5 4,267,883 - tolB BNILDI_21235 0.77 -3.2 4,267,883 - tolB BNILDI_21235 0.77 -3.2 4,267,883 - tolB BNILDI_21235 0.77 -3.1 4,267,967 - tolB BNILDI_21235 0.84 -2.3 4,268,164 + -2.0 4,268,181 + -2.6 4,268,182 - -0.5 4,268,182 - -0.5 4,268,251 + +0.3 4,268,252 - +0.2 4,268,313 - -0.1 4,268,504 + tolA BNILDI_21240 0.15 -6.1 4,268,505 - tolA BNILDI_21240 0.15 +2.1 4,268,520 - tolA BNILDI_21240 0.16 -3.4 4,268,520 - tolA BNILDI_21240 0.16 -5.7 4,268,563 - tolA BNILDI_21240 0.19 -2.0 4,268,595 + tolA BNILDI_21240 0.22 -0.9 4,268,596 - tolA BNILDI_21240 0.22 -1.4 4,269,150 + tolA BNILDI_21240 0.65 -2.9 4,269,419 + tolA BNILDI_21240 0.85 -5.4 4,269,419 + tolA BNILDI_21240 0.85 -3.9 4,269,497 - +0.3 4,269,562 - -4.6 4,269,675 + -0.7 4,269,675 + -1.3 4,269,693 - +0.2 4,269,723 - tolR BNILDI_21245 0.11 -4.8 4,269,744 - tolR BNILDI_21245 0.16 -1.1 4,270,225 - tolQ BNILDI_21250 0.17 -0.9 4,270,384 - tolQ BNILDI_21250 0.40 -3.3 4,270,459 + tolQ BNILDI_21250 0.51 -2.2 4,270,466 - tolQ BNILDI_21250 0.52 -2.9
Or see this region's nucleotide sequence