Strain Fitness in Escherichia coli ECRC62 around BNILDI_21240

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolB and tolA are separated by 132 nucleotidestolA and tolR are separated by 64 nucleotidestolR and tolQ are separated by 3 nucleotides BNILDI_21235: tolB - Tol-Pal system protein TolB, at 4,266,886 to 4,268,178 tolB BNILDI_21240: tolA - cell envelope integrity protein TolA, at 4,268,311 to 4,269,609 tolA BNILDI_21245: tolR - colicin uptake protein TolR, at 4,269,674 to 4,270,102 tolR BNILDI_21250: tolQ - Tol-Pal system protein TolQ, at 4,270,106 to 4,270,798 tolQ Position (kb) 4268 4269 4270Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1 2at 4267.368 kb on - strand, within tolBat 4267.471 kb on + strand, within tolBat 4267.674 kb on - strand, within tolBat 4267.680 kb on + strand, within tolBat 4267.751 kb on + strand, within tolBat 4267.795 kb on + strand, within tolBat 4267.796 kb on - strand, within tolBat 4267.845 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.883 kb on - strand, within tolBat 4267.967 kb on - strand, within tolBat 4268.164 kb on + strandat 4268.181 kb on + strandat 4268.182 kb on - strandat 4268.182 kb on - strandat 4268.251 kb on + strandat 4268.252 kb on - strandat 4268.313 kb on - strandat 4268.504 kb on + strand, within tolAat 4268.505 kb on - strand, within tolAat 4268.520 kb on - strand, within tolAat 4268.520 kb on - strand, within tolAat 4268.563 kb on - strand, within tolAat 4268.595 kb on + strand, within tolAat 4268.596 kb on - strand, within tolAat 4269.150 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.419 kb on + strand, within tolAat 4269.497 kb on - strandat 4269.562 kb on - strandat 4269.675 kb on + strandat 4269.675 kb on + strandat 4269.693 kb on - strandat 4269.723 kb on - strand, within tolRat 4269.744 kb on - strand, within tolRat 4270.225 kb on - strand, within tolQat 4270.384 kb on - strand, within tolQat 4270.459 kb on + strand, within tolQat 4270.466 kb on - strand, within tolQ

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,267,368 - tolB BNILDI_21235 0.37 -4.3
4,267,471 + tolB BNILDI_21235 0.45 -3.4
4,267,674 - tolB BNILDI_21235 0.61 -1.7
4,267,680 + tolB BNILDI_21235 0.61 -2.4
4,267,751 + tolB BNILDI_21235 0.67 -1.1
4,267,795 + tolB BNILDI_21235 0.70 -2.1
4,267,796 - tolB BNILDI_21235 0.70 -0.2
4,267,845 - tolB BNILDI_21235 0.74 +0.5
4,267,883 - tolB BNILDI_21235 0.77 -3.2
4,267,883 - tolB BNILDI_21235 0.77 -3.2
4,267,883 - tolB BNILDI_21235 0.77 -3.1
4,267,967 - tolB BNILDI_21235 0.84 -2.3
4,268,164 + -2.0
4,268,181 + -2.6
4,268,182 - -0.5
4,268,182 - -0.5
4,268,251 + +0.3
4,268,252 - +0.2
4,268,313 - -0.1
4,268,504 + tolA BNILDI_21240 0.15 -6.1
4,268,505 - tolA BNILDI_21240 0.15 +2.1
4,268,520 - tolA BNILDI_21240 0.16 -3.4
4,268,520 - tolA BNILDI_21240 0.16 -5.7
4,268,563 - tolA BNILDI_21240 0.19 -2.0
4,268,595 + tolA BNILDI_21240 0.22 -0.9
4,268,596 - tolA BNILDI_21240 0.22 -1.4
4,269,150 + tolA BNILDI_21240 0.65 -2.9
4,269,419 + tolA BNILDI_21240 0.85 -5.4
4,269,419 + tolA BNILDI_21240 0.85 -3.9
4,269,497 - +0.3
4,269,562 - -4.6
4,269,675 + -0.7
4,269,675 + -1.3
4,269,693 - +0.2
4,269,723 - tolR BNILDI_21245 0.11 -4.8
4,269,744 - tolR BNILDI_21245 0.16 -1.1
4,270,225 - tolQ BNILDI_21250 0.17 -0.9
4,270,384 - tolQ BNILDI_21250 0.40 -3.3
4,270,459 + tolQ BNILDI_21250 0.51 -2.2
4,270,466 - tolQ BNILDI_21250 0.52 -2.9

Or see this region's nucleotide sequence